Optineurin | Ubiquitylation Resource

Plasmid

pCMV5-HA-1 Optineurin E50K D474N

Parent Plasmid

pCMV5

Expressed

HA-Optineurin E50K D474N

DU Number

DU4803

Genbank

AF420371

Tag

HA

Species

Human

Sequence of Insert

View Sequence

GCCACCATGTACCCATACGATGTGCCAGATTACGCCGGATCCATGTCCCA
TCAACCTCTCAGCTGCCTCACTGAAAAGGAGGACAGCCCCAGTGAAAGCA
CAGGAAATGGACCCCCCCACCTGGCCCACCCAAACCTGGACACGTTTACC
CCGGAGGAGCTGCTGCAGCAGATGAAAGAGCTCCTGACCAAGAACCACCA
GCTGAAAGAAGCCATGAAGCTAAATAATCAAGCCATGAAAGGGAGATTTG
AGGAGCTTTCGGCCTGGACAGAGAAACAGAAGGAAGAACGCCAGTTTTTT
GAGATACAGAGCAAAGAAGCAAAAGAGCGTCTAATGGCCTTGAGTCATGA
GAATGAGAAATTGAAGGAAGAGCTTGGAAAACTAAAAGGGAAATCAGAAA
GGTCATCTGAGGACCCCACTGATGACTCCAGGCTTCCCAGGGCCGAAGCG
GAGCAGGAAAAGGACCAGCTCAGGACCCAGGTGGTGAGGCTACAAGCAGA
GAAGGCAGACCTGTTGGGCATCGTGTCTGAACTGCAGCTCAAGCTGAACT
CCAGCGGCTCCTCAGAAGATTCCTTTGTTGAAATTAGGATGGCTGAAGGA
GAAGCAGAAGGGTCAGTAAAAGAAATCAAGCATAGTCCTGGGCCCACGAG
AACAGTCTCCACTGGCACGGCATTGTCTAAATATAGGAGCAGATCTGCAG
ATGGGGCCAAGAATTACTTCGAACATGAGGAGTTAACTGTGAGCCAGCTC
CTGCTGTGCCTAAGGGAAGGGAATCAGAAGGTGGAGAGACTTGAAGTTGC
ACTCAAGGAGGCCAAAGAAAGAGTTTCAGATTTTGAAAAGAAAACAAGTA
ATCGTTCTGAGATTGAAACCCAGACAGAGGGGAGCACAGAGAAAGAGAAT
GATGAAGAGAAAGGCCCGGAGACTGTTGGAAGCGAAGTGGAAGCACTGAA
CCTCCAGGTGACATCTCTGTTTAAGGAGCTTCAAGAGGCTCATACAAAAC
TCAGCGAAGCTGAGCTAATGAAGAAGAGACTTCAAGAAAAGTGTCAGGCC
CTTGAAAGGAAAAATTCTGCAATTCCATCAGAGTTGAATGAAAAGCAAGA
GCTTGTTTATACTAACAAAAAGTTAGAGCTACAAGTGGAAAGCATGCTAT
CAGAAATCAAAATGGAACAGGCTAAAACAGAGGATGAAAAGTCCAAATTA
ACTGTGCTACAGATGACACACAACAAGCTTCTTCAAGAACATAATAATGC
ATTGAAAACAATTGAGGAACTAACAAGAAAAGAGTCAGAAAAAGTGGACA
GGGCAGTGCTGAAGGAACTGAGTGAAAAACTGGAACTGGCAGAGAAGGCT
CTGGCTTCCAAACAGCTGCAAATGGATGAAATGAAGCAAACCATTGCCAA
GCAGGAAGAGGACCTGGAAACCATGACCATCCTCAGGGCTCAGATGGAAG
TTTACTGTTCTAATTTTCATGCTGAAAGAGCAGCGAGAGAGAAAATTCAT
GAGGAAAAGGAGCAACTGGCATTGCAGCTGGCAGTTCTGCTGAAAGAGAA
TGATGCTTTCGAAGACGGAGGCAGGCAGTCCTTGATGGAGATGCAGAGTC
GTCATGGGGCGAGAACAAGTGACTCTGACCAGCAGGCTTACCTTGTTCAA
AGAGGAGCTGAGGACAGGGACTGGCGGCAACAGCGGAATATTCCGATTCA
TTCCTGCCCCAAGTGTGGAGAGGTTCTGCCTGACATAGACACGTTACAGA
TTCACGTGATGGATTGCATCATTTAAGCGGCCGCG

Amino Acid Sequence

View Sequence

MYPYDVPDYAGSMSHQPLSCLTEKEDSPSESTGNGPPHLAHPNLDTFTPE
ELLQQMKELLTKNHQLKEAMKLNNQAMKGRFEELSAWTEKQKEERQFFEI
QSKEAKERLMALSHENEKLKEELGKLKGKSERSSEDPTDDSRLPRAEAEQ
EKDQLRTQVVRLQAEKADLLGIVSELQLKLNSSGSSEDSFVEIRMAEGEA
EGSVKEIKHSPGPTRTVSTGTALSKYRSRSADGAKNYFEHEELTVSQLLL
CLREGNQKVERLEVALKEAKERVSDFEKKTSNRSEIETQTEGSTEKENDE
EKGPETVGSEVEALNLQVTSLFKELQEAHTKLSEAELMKKRLQEKCQALE
RKNSAIPSELNEKQELVYTNKKLELQVESMLSEIKMEQAKTEDEKSKLTV
LQMTHNKLLQEHNNALKTIEELTRKESEKVDRAVLKELSEKLELAEKALA
SKQLQMDEMKQTIAKQEEDLETMTILRAQMEVYCSNFHAERAAREKIHEE
KEQLALQLAVLLKENDAFEDGGRQSLMEMQSRHGARTSDSDQQAYLVQRG
AEDRDWRQQRNIPIHSCPKCGEVLPDIDTLQIHVMDCII*

Vector Type

Mammalian

Antibiotic

Amp

Comments

Mammalian expression with N term HA.

100042 R 1000 1001 1047 19007