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Plasmid
pCMV5-HA ITCH
Parent Plasmid
pCMV5 HA1
Expressed
HA-ITCH
DU Number
DU31129
Genbank
NM_031483
Tag
HA
Species
Human
Sequence of Insert
GGATCCATGTCTGACAGTGGATCACAACTTGGTTCAATGGGTAGCCTCACCATGAAATCACAGCTTCAGATCACTGTCATCTCAGCAAAACTTAAGGAAAATAAGAAGAATTGGTTTGGACCAAGTCCTTACGTAGAGGTCACAGTAGATGGACAGTCAAAGAAGACAGAAAAATGCAACAACACAAACAGTCCCAAGTGGAAGCAACCCCTTACAGTTATCGTTACCCCTGTGAGTAAATTACATTTTCGTGTGTGGAGTCACCAGACACTGAAATCTGATGTTTTGTTGGGAACTGCTGCATTA
GATATTTATGAAACATTAAAGTCAAACAATATGAAACTTGAAGAAGTAGT
TGTGACTTTGCAGCTTGGAGGTGACAAAGAGCCAACAGAGACAATAGGAG
ACTTGTCAATTTGTCTTGATGGGCTACAGTTAGAGTCTGAAGTTGTTACC
AATGGTGAAACTACATGTTCAGAAAGTGCTTCTCAGAATGATGATGGCTC
CAGATCCAAGGATGAAACAAGAGTGAGCACAAATGGATCAGATGACCCTG
AAGATGCAGGAGCTGGTGAAAATAGGAGAGTCAGTGGGAATAATTCTCCA
TCACTCTCAAATGGTGGTTTTAAACCTTCTAGACCTCCAAGACCTTCACG
ACCACCACCACCCACCCCACGTAGACCAGCATCTGTCAATGGTTCACCAT
CTGCCACTTCTGAAAGTGATGGGTCTAGTACAGGCTCTCTGCCGCCGACA
AATACAAATACAAATACATCTGAAGGAGCAACATCTGGATTAATAATTCC
TCTTACTATATCTGGAGGCTCAGGCCCTAGGCCATTAAATCCTGTAACTC
AAGCTCCCTTGCCACCTGGTTGGGAGCAGAGAGTGGACCAGCACGGGCGA
GTTTACTATGTAGATCATGTTGAGAAAAGAACAACATGGGATAGACCAGA
ACCTCTACCTCCTGGCTGGGAACGGCGGGTTGACAACATGGGACGTATTT
ATTATGTTGACCATTTCACAAGAACAACAACGTGGCAGAGGCCAACACTG
GAATCCGTCCGGAACTATGAACAATGGCAGCTACAGCGTAGTCAGCTTCA
AGGAGCAATGCAGCAGTTTAACCAGAGATTCATTTATGGGAATCAAGATT
TATTTGCTACATCACAAAGTAAAGAATTTGATCCTCTTGGTCCATTGCCA
CCTGGATGGGAGAAGAGAACAGACAGCAATGGCAGAGTATATTTCGTCAA
CCACAACACACGAATTACACAATGGGAAGACCCCAGAAGTCAAGGTCAAT
TAAATGAAAAGCCCTTACCTGAAGGTTGGGAAATGAGATTCACAGTGGAT
GGAATTCCATATTTTGTGGACCACAATAGAAGAACTACCACCTATATAGA
TCCCCGCACAGGAAAATCTGCCCTAGACAATGGACCTCAGATAGCCTATG
TTCGGGACTTCAAAGCAAAGGTTCAGTATTTCCGGTTCTGGTGTCAGCAA
CTGGCCATGCCACAGCACATAAAGATTACAGTGACAAGAAAAACATTGTT
TGAGGATTCCTTTCAACAGATAATGAGCTTCAGTCCCCAAGATCTGCGAA
GACGTTTGTGGGTGATTTTTCCAGGAGAAGAAGGTTTAGATTATGGAGGT
GTAGCAAGAGAATGGTTCTTTCTTTTGTCACATGAAGTGTTGAACCCAAT
GTATTGCCTGTTTGAATATGCAGGGAAGGATAACTACTGCTTGCAGATAA
ACCCCGCTTCTTACATCAATCCAGATCACCTGAAATATTTTCGTTTTATT
GGCAGATTCATTGCCATGGCTCTGTTCCATGGGAAATTCATAGACACGGG
TTTTTCTTTACCATTCTATAAGCGTATCTTGAACAAACCAGTTGGACTCA
AGGATTTAGAATCTATTGATCCAGAATTTTACAATTCTCTCATCTGGGTT
AAGGAAAACAATATTGAGGAATGTGATTTGGAAATGTACTTCTCCGTTGA
CAAAGAAATTCTAGGTGAAATTAAGAGTCATGATCTGAAACCTAATGGTG
GCAATATTCTTGTAACAGAAGAAAATAAAGAGGAATACATCAGAATGGTA
GCTGAGTGGAGGTTGTCTCGAGGTGTTGAAGAACAGACACAAGCTTTCTT
TGAAGGCTTTAATGAAATTCTTCCCCAGCAATATTTGCAATACTTTGATG
CAAAGGAATTAGAGGTCCTTTTATGTGGAATGCAAGAGATTGATTTGAAT
GACTGGCAAAGACATGCCATCTACCGTCATTATGCAAGGACCAGCAAACA
AATCATGTGGTTTTGGCAGTTTGTTAAAGAAATTGATAATGAGAAGAGAA
TGAGACTTCTGCAGTTTGTTACTGGAACCTGCCGATTGCCAGTAGGAGGA
TTTGCTGATCTCATGGGGAGCAATGGACCACAGAAATTCTGCATTGAAAA
AGTTGGGAAAGAAAATTGGCTACCCAGAAGTCATACCTGTTTTAATCGCC
TGGACCTGCCACCATACAAGAGCTATGAGCAACTGAAGGAAAAGCTGTTG
TTTGCCATAGAAGAAACAGAAGGATTTGGACAAGAGTAAGCGGCCGC
Amino Acid Sequence
MYPYDVPDYAGSMSDSGSQLGSMGSLTMKSQLQITVISAKLKENKKNWFG
PSPYVEVTVDGQSKKTEKCNNTNSPKWKQPLTVIVTPVSKLHFRVWSHQT
LKSDVLLGTAALDIYETLKSNNMKLEEVVVTLQLGGDKEPTETIGDLSIC
LDGLQLESEVVTNGETTCSESASQNDDGSRSKDETRVSTNGSDDPEDAGA
GENRRVSGNNSPSLSNGGFKPSRPPRPSRPPPPTPRRPASVNGSPSATSE
SDGSSTGSLPPTNTNTNTSEGATSGLIIPLTISGGSGPRPLNPVTQAPLP
PGWEQRVDQHGRVYYVDHVEKRTTWDRPEPLPPGWERRVDNMGRIYYVDH
FTRTTTWQRPTLESVRNYEQWQLQRSQLQGAMQQFNQRFIYGNQDLFATS
QSKEFDPLGPLPPGWEKRTDSNGRVYFVNHNTRITQWEDPRSQGQLNEKP
LPEGWEMRFTVDGIPYFVDHNRRTTTYIDPRTGKSALDNGPQIAYVRDFK
AKVQYFRFWCQQLAMPQHIKITVTRKTLFEDSFQQIMSFSPQDLRRRLWV
IFPGEEGLDYGGVAREWFFLLSHEVLNPMYCLFEYAGKDNYCLQINPASY
INPDHLKYFRFIGRFIAMALFHGKFIDTGFSLPFYKRILNKPVGLKDLES
IDPEFYNSLIWVKENNIEECDLEMYFSVDKEILGEIKSHDLKPNGGNILV
TEENKEEYIRMVAEWRLSRGVEEQTQAFFEGFNEILPQQYLQYFDAKELE
VLLCGMQEIDLNDWQRHAIYRHYARTSKQIMWFWQFVKEIDNEKRMRLLQ
FVTGTCRLPVGGFADLMGSNGPQKFCIEKVGKENWLPRSHTCFNRLDLPP
YKSYEQLKEKLLFAIEETEGFGQE*
Vector Type
Mammalian
Antibiotic
Amp
Comments
Estimated MW of expressed protein : 100033 Daltons
100042 R 1000 1001 1047 19007
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