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Plasmid
pGEX6P-1-Ubiquitin K29wt K33wt
Parent Plasmid
pGEX6P1
Expressed
GST-Ubiquitin K29wt K33wt
DU Number
DU8582
Genbank
P62988
Tag
GST
Sequence of Insert
View Sequence
GGATCTCAAATCCCCCTGAGGACCCTGACTGGTAGGACCATCACTCTCGA
AGTGGAGCCGAGTGACACCATTGAGAATGTCAGGGCAAAGATCCAAGACA
AGGAAGGCATCCCTCCTGACCAGCAGAGGTTGATCTTTGCTGGGAGACAG
CTGGAAGATGGACGCACCCTGTCTGACTACAACATCCAGAGAGAGTCCAC
CCTGCACCTGGTCCTCCGTCTCAGAGGTGGGTGATAACTCGACTCGAGCG
GCCGC
AGTGGAGCCGAGTGACACCATTGAGAATGTCAGGGCAAAGATCCAAGACA
AGGAAGGCATCCCTCCTGACCAGCAGAGGTTGATCTTTGCTGGGAGACAG
CTGGAAGATGGACGCACCCTGTCTGACTACAACATCCAGAGAGAGTCCAC
CCTGCACCTGGTCCTCCGTCTCAGAGGTGGGTGATAACTCGACTCGAGCG
GCCGC
Amino Acid Sequence
View Sequence
MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSQIPLRTLTGRTITLEVEPS
DTIENVRAKIQDKEGIPPDQQRLIFAGRQLEDGRTLSDYNIQRESTLHLV
LRLRGG*
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSQIPLRTLTGRTITLEVEPS
DTIENVRAKIQDKEGIPPDQQRLIFAGRQLEDGRTLSDYNIQRESTLHLV
LRLRGG*
Vector Type
Bacterial
Antibiotic
Amp
Comments
N terminal GST/FLAG for bacterial expression
100042 R 1000 1001 1047 19007
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