TRAFD1 | Ubiquitylation Resource

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Plasmid

pEBG6P-TRAFD1

Parent Plasmid

pEBG6P

Expressed

GST TRAFD1

DU Number

DU35545

Genbank

NM_006700.1

Tag

GST

Species

Human

Sequence of Insert

View Sequence

GGATCCATGGCTGAATTTCTAGATGACCAGGAAACTCGACTGTGTGACAA
CTGCAAAAAAGAAATTCCTGTGTTTAACTTTACCATCCATGAGATCCACT
GTCAAAGGAACATTGGTATGTGTCCTACCTGTAAGGAACCATTTCCCAAA
TCTGACATGGAGACTCACATGGCTGCAGAACACTGTCAGGTGACCTGCAA
ATGTAACAAGAAGTTGGAGAAGAGGCTGTTAAAGAAGCATGAGGAGACTG
AGTGCCCTTTGCGGCTTGCTGTCTGCCAGCACTGTGATTTAGAACTTTCC
ATTCTCAAACTGAAGGAACATGAAGATTATTGTGGTGCCCGGACGGAACT
ATGTGGCAACTGTGGTCGCAATGTCCTTGTGAAAGATCTGAAGACTCACC
CTGAAGTTTGTGGGAGAGAGGGGGAGGAAAAGAGAAATGAGGTTGCCATA
CCTCCTAATGCATATGATGAATCTTGGGGTCAGGATGGAATCTGGATTGC
ATCCCAACTCCTCAGACAAATTGAGGCTCTGGACCCACCCATGAGGCTGC
CGCGAAGGCCCCTGAGAGCCTTTGAATCAGATGTTTTCCACAATAGAACT
ACCAACCAAAGGAACATTACAGCCCAGGTTTCAATTCAGAATAATCTGTT
TGAAGAACAAGAGAGGCAGGAAAGGAATAGAGGCCAACAGCCCCCCAAAG
AGGGTGGTGAAGAGAGTGCAAACTTGGACTTCATGTTGGCCCTAAGTCTG
CAAAATGAAGGCCAAGCCTCCAGTGTGGCAGAGCAGGACTTCTGGAGGGC
CGTATGTGAGGCCGACCAGTCTCATGGCGGTCCCAGGTCTCTCAGTGACA
TAAAGGGTGCAGCTGACGAGATCATGTTGCCTTGTGAATTTTGTGAGGAG
CTCTACCCAGAGGAACTGCTGATTGACCATCAGACAAGCTGTAACCCTTC
ACGTGCCTTACCTTCACTCAATACTGGCAGCTCTTCCCCCAGAGGGGTGG
AGGAACCTGATGTCATCTTCCAGAACTTCTTGCAACAGGCTGCAAGTAAC
CAGTTAGACTCTTTGATGGGCCTGAGCAATTCACACCCTGTGGAGGAGAG
CATCATTATCCCATGTGAATTCTGTGGGGTACAGCTGGAAGAGGAGGTGC
TGTTCCATCACCAGGACCAGTGTGACCAACGCCCAGCCACTGCAACCAAC
CATGTGACAGAGGGGATTCCTAGACTGGATTCCCAGCCTCAAGAGACCTC
ACCAGAGCTGCCCAGGAGGCGTGTCAGACACCAGGGAGACCTGTCTTCTG
GTTACCTGGATGATACTAAGCAGGAAACAGCTAATGGGCCCACCTCCTGT
CTGCCTCCCAGCCGACCCATTAACAATATGACAGCTACCTATAACCAGCT
ATCGAGATCAACATCAGGCCCCAGACCTGGGTGCCAGCCCAGCTCTCCTT
GTGTGCCGAAGCTCAGCAACTCAGACAGCCAGGACATCCAGGGGCGGAAT
CGAGACAGCCAGAATGGGGCCATAGCCCCTGGGCACGTTTCAGTGATTCG
CCCTCCTCAAAATCTCTACCCAGAAAACATTGTGCCCTCTTTCTCCCCTG
GGCCTTCAGGGAGATACGGAGCTAGTGGTAGGAGTGAAGGTGGCAGGAAT
TCCCGGGTCACCCCTGCAGCTGCCAACTACCGCAGCAGAACTGCAAAGGC
AAAGCCTTCCAAGCAACAGGGAGCTGGGGATGCAGAAGAGGAAGAGGAGG
AGTAAGCGGCCGC

Amino Acid Sequence

View Sequence

MAPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSMAEFLDDQETRLC
DNCKKEIPVFNFTIHEIHCQRNIGMCPTCKEPFPKSDMETHMAAEHCQVT
CKCNKKLEKRLLKKHEETECPLRLAVCQHCDLELSILKLKEHEDYCGART
ELCGNCGRNVLVKDLKTHPEVCGREGEEKRNEVAIPPNAYDESWGQDGIW
IASQLLRQIEALDPPMRLPRRPLRAFESDVFHNRTTNQRNITAQVSIQNN
LFEEQERQERNRGQQPPKEGGEESANLDFMLALSLQNEGQASSVAEQDFW
RAVCEADQSHGGPRSLSDIKGAADEIMLPCEFCEELYPEELLIDHQTSCN
PSRALPSLNTGSSSPRGVEEPDVIFQNFLQQAASNQLDSLMGLSNSHPVE
ESIIIPCEFCGVQLEEEVLFHHQDQCDQRPATATNHVTEGIPRLDSQPQE
TSPELPRRRVRHQGDLSSGYLDDTKQETANGPTSCLPPSRPINNMTATYN
QLSRSTSGPRPGCQPSSPCVPKLSNSDSQDIQGRNRDSQNGAIAPGHVSV
IRPPQNLYPENIVPSFSPGPSGRYGASGRSEGGRNSRVTPAAANYRSRTA
KAKPSKQQGAGDAEEEEEE*

Vector Type

Mammalian

Antibiotic

Amp

Comments

N ter GST for mammalian expression

Estimated MW of expressed protein : 64983 Daltons

100042 R 1000 1001 1047 19007

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