USP20 | Ubiquitylation Resource

This reagent and more can be found at our sister site MRC PPU Reagents & Services

Request Now

Plasmid

pGEX6P-1-USP20

Parent Plasmid

pGEX6P1

Expressed

GST-USP20

DU Number

DU12807

Genbank

AAH39593.1

Tag

GST

Species

Human

Sequence of Insert

View Sequence

GGATCCATGGGGGACTCCAGGGACCTTTGCCCTCACCTTGACTCCATAGG
AGAGGTGACCAAAGAGGACTTGCTGCTCAAATCTAAGGGAACCTGTCAGT
CGTGTGGGGTCACCGGACCAAACCTATGGGCCTGTCTGCAGGTTGCCTGC
CCCTATGTTGGCTGCGGAGAATCCTTCGCTGACCACAGCACCATTCATGC
ACAGGCAAAAAAGCACAACTTGACCGTGAACCTGACCACGTTCCGACTGT
GGTGTTACGCCTGTGAGAAGGAGGTATTCCTGGAGCAGCGGCTGGCAGCC
CCTCTGCTGGGCTCCTCTTCCAAGTTCTCTGAACAGGACTCCCCGCCACC
CTCCCACCCTCTGAAAGCTGTTCCTATTGCTGTGGCTGATGAAGGAGAGT
CTGAGTCAGAGGATGATGACCTGAAACCTCGAGGCCTCACGGGCATGAAG
AACCTCGGGAACTCCTGCTACATGAACGCCGCCCTGCAGGCCCTGTCCAA
TTGCCCGCCGCTGACTCAGTTCTTCTTGGAGTGTGGCGGCCTGGTGCGCA
CAGATAAGAAGCCAGCCCTGTGCAAGAGCTACCAGAAGCTGGTCTCTGAG
GTCTGGCATAAGAAACGGCCAAGCTACGTGGTCCCCACCAGTCTGTCTCA
TGGGATCAAGTTGGTCAACCCAATGTTCCGAGGCTATGCCCAGCAGGACA
CCCAAGAGTTCCTTCGCTGCCTGATGGACCAGCTGCACGAGGAGCTCAAG
GAGCCGGTGGTGGCCACGGTGGCGCTGACGGAGGCTCGGGACTCAGATTC
GAGTGACACGGATGAGAAACGGGAGGGTGACCGGAGCCCATCAGAAGATG
AGTTCTTGTCCTGTGACTCGAGCAGTGACCGGGGTGAGGGTGACGGGCAG
GGGCGTGGCGGGGGCAGCTCGCAGGCCGAGACGGAGCTGCTGATCCCAGA
TGAGGCGGGCCGAGCCATCTCTGAGAAGGAGCGGATGAAGGACCGCAAGT
TCTCCTGGGGCCAGCAGCGTACAAACTCGGAGCAAGTGGACGAGGACGCT
GATGTGGACACTGCCATGGCTGCCCTTGACCAGCCCGCGGAGGCCCAGCC
CCCGTCACCACGGTCCTCCAGCCCCTGCCGGACGCCAGAGCCGGACAATG
ATGCTCACCTACGCAGCTCCTCTCGCCCCTGCAGCCCCGTCCACCACCAC
GAGGGCCATGCCAAGCTGTCTAGCAGCCCCCCTCGTGCAAGCCCCGTGAG
GATGGCACCGTCGTACGTGCTCAAGAAAGCCCAGGTATTGAGTGCTGGCA
GCCGGAGGCGGAAGGAGCAGCGCTACCGCAGCGTCATCTCAGACATCTTT
GACGGCTCCATTCTCAGCCTCGTGCAGTGTCTCACCTGTGACCGGGTATC
CACCACAGTGGAAACGTTCCAGGACTTATCACTGCCCATTCCTGGAAAGG
AGGACCTGGCCAAGCTCCATTCAGCCATCTACCAGAATGTGCCGGCCAAG
CCAGGCGCCTGTGGGGACAGCTATGCCGCCCAGGGCTGGCTGGCCTTCAT
TGTGGAGTACATCCGACGGTTTGTGGTATCCTGTACCCCCAGCTGGTTTT
GGGGGCCTGTCGTCACCCTGGAAGACTGCCTTGCTGCCTTCTTTGCCGCT
GATGAGTTAAAGGGTGACAACATGTACAGCTGTGAGCGGTGTAAGAAGCT
GCGGAACGGAGTGAAGTACTGCAAAGTCCTGCGGTTGCCCGAGATCCTGT
GCATTCACCTAAAGCGCTTTCGGCACGAGGTGATGTACTCATTCAAGATC
AACAGCCACGTCTCCTTCCCCCTCGAGGGGCTCGACCTGCGCCCCTTCCT
TGCCAAGGAGTGCACATCCCAGATCACCACCTACGACCTCCTCTCGGTCA
TCTGCCACCACGGCACGGCAGGCAGTGGGCACTACATCGCCTACTGCCAG
AACGTGATCAATGGGCAGTGGTACGAGTTTGATGACCAGTACGTCACAGA
AGTCCACGAGACGGTGGTGCAGAACGCCGAGGGCTACGTACTCTTCTACA
GGAAGAGCAGCGAGGAGGCCATGCGGGAGCGACAGCAGGTGGTGTCCCTG
GCCGCCATGCGGGAGCCCAGCCTGCTGCGGTTCTACGTGTCCCGCGAGTG
GCTCAACAAGTTCAACACCTTCGCGGAGCCAGGCCCCATCACCAACCAGA
CCTTCCTCTGCTCCCACGGAGGCATCCCGCCCCACAAATACCACTACATC
GACGACCTGGTGGTCATCCTGCCCCAGAACGTCTGGGAGCACCTGTACAA
CAGATTCGGGGGTGGCCCCGCCGTGAACCACCTGTACGTGTGCTCCATCT
GCCAGGTGGAGATCGAGGCACTGGCCAAGCGCAGGAGGATCGAGATCGAC
ACCTTCATCAAGTTGAACAAGGCCTTCCAGGCCGAGGAGTCGCCGGGCGT
CATCTACTGCATCAGCATGCAGTGGTTCCGGGAGTGGGAGGCGTTCGTCA
AGGGGAAGGACAACGAGCCCCCCGGGCCCATTGACAACAGCAGGATTGCA
CAGGTCAAAGGAAGCGGCCATGTCCAGCTGAAGCAGGGAGCTGACTACGG
GCAGATTTCGGAGGAGACCTGGACCTACCTGAACAGCCTGTATGGAGGTG
GCCCCGAGATTGCCATCCGCCAGAGTGTGGCGCAGCCGCTGGGCCCAGAG
AACCTGCACGGGGAGCAGAAGATCGAAGCCGAGACGCGGGCCGTGTGAGC
GGCCGC

Amino Acid Sequence

View Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMGDSRDLCPHLDSIGEVTK
EDLLLKSKGTCQSCGVTGPNLWACLQVACPYVGCGESFADHSTIHAQAKK
HNLTVNLTTFRLWCYACEKEVFLEQRLAAPLLGSSSKFSEQDSPPPSHPL
KAVPIAVADEGESESEDDDLKPRGLTGMKNLGNSCYMNAALQALSNCPPL
TQFFLECGGLVRTDKKPALCKSYQKLVSEVWHKKRPSYVVPTSLSHGIKL
VNPMFRGYAQQDTQEFLRCLMDQLHEELKEPVVATVALTEARDSDSSDTD
EKREGDRSPSEDEFLSCDSSSDRGEGDGQGRGGGSSQAETELLIPDEAGR
AISEKERMKDRKFSWGQQRTNSEQVDEDADVDTAMAALDQPAEAQPPSPR
SSSPCRTPEPDNDAHLRSSSRPCSPVHHHEGHAKLSSSPPRASPVRMAPS
YVLKKAQVLSAGSRRRKEQRYRSVISDIFDGSILSLVQCLTCDRVSTTVE
TFQDLSLPIPGKEDLAKLHSAIYQNVPAKPGACGDSYAAQGWLAFIVEYI
RRFVVSCTPSWFWGPVVTLEDCLAAFFAADELKGDNMYSCERCKKLRNGV
KYCKVLRLPEILCIHLKRFRHEVMYSFKINSHVSFPLEGLDLRPFLAKEC
TSQITTYDLLSVICHHGTAGSGHYIAYCQNVINGQWYEFDDQYVTEVHET
VVQNAEGYVLFYRKSSEEAMRERQQVVSLAAMREPSLLRFYVSREWLNKF
NTFAEPGPITNQTFLCSHGGIPPHKYHYIDDLVVILPQNVWEHLYNRFGG
GPAVNHLYVCSICQVEIEALAKRRRIEIDTFIKLNKAFQAEESPGVIYCI
SMQWFREWEAFVKGKDNEPPGPIDNSRIAQVKGSGHVQLKQGADYGQISE
ETWTYLNSLYGGGPEIAIRQSVAQPLGPENLHGEQKIEAETRAV

Vector Type

Bacterial

Antibiotic

Amp

Comments

N ter GST for bacterial expression.

Estimated MW of expressed protein : 128708 Daltons

100042 R 1000 1001 1047 19007

Download datasheet