BAP1 | Ubiquitylation Resource

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Plasmid

pCMV-FLAG-1 BAP1

Parent Plasmid

pCMV5 FLAG1

Expressed

FLAG BAP1

DU Number

DU12781

Genbank

AAH01596.1

Tag

FLAG

Species

Human

Sequence of Insert

View Sequence

GGATCCATGAATAAGGGCTGGCTGGAGCTGGAGAGCGACCCAGGCCTCTT
CACCCTGCTCGTGGAAGATTTCGGTGTCAAGGGGGTGCAAGTGGAGGAGA
TCTACGACCTTCAGAGCAAATGTCAGGGCCCTGTATATGGATTTATCTTC
CTGTTCAAATGGATCGAAGAGCGCCGGTCCCGGCGAAAGGTCTCTACCTT
GGTGGATGATACGTCCGTGATTGATGATGATATTGTGAATAACATGTTCT
TTGCCCACCAGCTGATACCCAACTCTTGTGCAACTCATGCCTTGCTGAGC
GTGCTCCTGAACTGCAGCAGCGTGGACCTGGGACCCACCCTGAGTCGCAT
GAAGGACTTCACCAAGGGTTTTAGCCCTGAGAGCAAAGGATATGCGATTG
GCAATGCCCCGGAGTTGGCCAAGGCCCATAATAGCCATGCCAGGCCCGAG
CCACGCCACCTCCCTGAGAAGCAGAATGGCCTTAGTGCAGTGCGGACCAT
GGAGGCGTTCCACTTTGTCAGCTATGTGCCTATCACAGGCCGGCTCTTTG
AGCTGGATGGGCTGAAGGTCTACCCCATTGACCATGGGCCCTGGGGGGAG
GACGAGGAGTGGACAGACAAGGCCCGGCGGGTCATCATGGAGCGTATCGG
CCTCGCCACTGCAGGGGAGCCCTACCACGACATCCGCTTCAACCTGATGG
CAGTGGTGCCCGACCGCAGGATCAAGTATGAGGCCAGGCTGCATGTGCTG
AAGGTGAACCGTCAGACAGTACTAGAGGCTCTGCAGCAGCTGATAAGAGT
AACACAGCCAGAGCTGATTCAGACCCACAAGTCTCAAGAGTCACAGCTGC
CTGAGGAGTCCAAGTCAGCCAGCAACAAGTCCCCGCTGGTGCTGGAAGCA
AACAGGGCCCCTGCAGCCTCTGAGGGCAACCACACAGATGGTGCAGAGGA
GGCGGCTGGTTCATGCGCACAAGCCCCATCCCACAGCCCTCCCAACAAAC
CCAAGCTAGTGGTGAAGCCTCCAGGCAGCAGCCTCAATGGGGTTCACCCC
AACCCCACTCCCATTGTCCAGCGGCTGCCGGCCTTTCTAGACAATCACAA
TTATGCCAAGTCCCCCATGCAGGAGGAAGAAGACCTGGCGGCAGGTGTGG
GCCGCAGCCGAGTTCCAGTCCGCCCACCCCAGCAGTACTCAGATGATGAG
GATGACTATGAGGATGACGAGGAGGATGACGTGCAGAACACCAACTCTGC
CCTTAGGTATAAGGGGAAGGGAACAGGGAAGCCAGGGGCATTGAGCGGTT
CTGCTGATGGGCAACTGTCAGTGCTGCAGCCCAACACCATCAACGTCTTG
GCTGAGAAGCTCAAAGAGTCCCAGAAGGACCTCTCAATTCCTCTGTCCAT
CAAGACTAGCAGCGGGGCTGGGAGTCCGGCTGTGGCAGTGCCCACACACT
CGCAGCCCTCACCCACCCCCAGCAATGAGAGTACAGACACGGCCTCTGAG
ATCGGCAGTGCTTTCAACTCGCCACTGCGCTCGCCTATCCGCTCAGCCAA
CCCGACGCGGCCCTCCAGCCCTGTCACCTCCCACATCTCCAAGGTGCTTT
TTGGAGAGGATGACAGCCTGCTGCGTGTTGACTGCATACGCTACAACCGT
GCTGTCCGTGATCTGGGTCCTGTCATCAGCACAGGCCTGCTGCACCTGGC
TGAGGATGGGGTGCTGAGTCCCCTGGCGCTGACAGAGGGTGGGAAGGGTT
CCTCGCCCTCCATCAGACCAATCCAAGGCAGCCAGGGGTCCAGCAGCCCA
GTGGAGAAGGAGGTCGTGGAAGCCACGGACAGCAGAGAGAAGACGGGGAT
GGTGAGGCCTGGCGAGCCCTTGAGTGGGGAGAAATACTCACCCAAGGAGC
TGCTGGCACTGCTGAAGTGTGTGGAGGCTGAGATTGCAAACTATGAGGCG
TGCCTCAAGGAGGAGGTAGAGAAGAGGAAGAAGTTCAAGATTGATGACCA
GAGAAGGACCCACAACTACGATGAGTTCATCTGCACCTTTATCTCCATGC
TGGCTCAGGAAGGCATGCTGGCCAACCTAGTGGAGCAGAACATCTCCGTG
CGGCGGCGCCAAGGGGTCAGCATCGGCCGGCTCCACAAGCAGCGGAAGCC
TGACCGGCGGAAACGCTCTCGCCCCTACAAGGCCAAGCGCCAGTGAGCGG
CCGC

Amino Acid Sequence

View Sequence

MDYKDDDDKGSMNKGWLELESDPGLFTLLVEDFGVKGVQVEEIYDLQSKC
QGPVYGFIFLFKWIEERRSRRKVSTLVDDTSVIDDDIVNNMFFAHQLIPN
SCATHALLSVLLNCSSVDLGPTLSRMKDFTKGFSPESKGYAIGNAPELAK
AHNSHARPEPRHLPEKQNGLSAVRTMEAFHFVSYVPITGRLFELDGLKVY
PIDHGPWGEDEEWTDKARRVIMERIGLATAGEPYHDIRFNLMAVVPDRRI
KYEARLHVLKVNRQTVLEALQQLIRVTQPELIQTHKSQESQLPEESKSAS
NKSPLVLEANRAPAASEGNHTDGAEEAAGSCAQAPSHSPPNKPKLVVKPP
GSSLNGVHPNPTPIVQRLPAFLDNHNYAKSPMQEEEDLAAGVGRSRVPVR
PPQQYSDDEDDYEDDEEDDVQNTNSALRYKGKGTGKPGALSGSADGQLSV
LQPNTINVLAEKLKESQKDLSIPLSIKTSSGAGSPAVAVPTHSQPSPTPS
NESTDTASEIGSAFNSPLRSPIRSANPTRPSSPVTSHISKVLFGEDDSLL
RVDCIRYNRAVRDLGPVISTGLLHLAEDGVLSPLALTEGGKGSSPSIRPI
QGSQGSSSPVEKEVVEATDSREKTGMVRPGEPLSGEKYSPKELLALLKCV
EAEIANYEACLKEEVEKRKKFKIDDQRRTHNYDEFICTFISMLAQEGMLA
NLVEQNISVRRRQGVSIGRLHKQRKPDRRKRSRPYKAKRQ*

Vector Type

Mammalian

Antibiotic

Amp

Comments

N ter FLAG for mammalian expression.

Estimated MW of expressed protein : 81629 Daltons

100042 R 1000 1001 1047 19007

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