UBE2NL | Ubiquitylation Resource

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Plasmid

pEBG6P-UBE2NL

Parent Plasmid

pEBG6P

Expressed

GST UBE2NL

DU Number

DU15983

Genbank

NP_001013007

Tag

GST

Species

Human

Sequence of Insert

View Sequence

GGATCCGCCGAGCTGCCCCACAGGATCATCAAGGAAACCCAGCGTTTGCT
GGCAGAACCAGTTCCTGGCATCAAAGCCGAACCAGATGAGAGCAACGCCC
GTTATTTTCATGTGGTCATTGCTGGGGAATCAAAGGATTCCCCCTTTGAG
GGAGGGACTTTTAAACGTGAACTATTACTTGCAGAAGAATACCCAATGGC
AGCCCCTAAAGTACGTTTCATGACCAAAATTTATCATCCTAATGTAGACA
AGTTGGAAAGAATAAGTTTAGATATTTTGAAAGATAAGTGGTCCCCAGCA
CTGCAGATCCGCACAGTTCTGCTATCGATCCAGGCCTTGTTAAATGCTCC
CAATCCAGATGATCCATTAGCAAATGATGTAGTGGAGCAGTGGAAGACCA
ACGAAGCCCAAGCCATAGAAACAGCTAGAGCATGGACTAGGCTATATGCC
ATGAATAGTATTTAAGAATTCCGAAGGGCGAATTCTGCAGATATCCATCA
CACTGGCGGCCGC

Amino Acid Sequence

View Sequence

MAPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSAELPHRIIKETQR
LLAEPVPGIKAEPDESNARYFHVVIAGESKDSPFEGGTFKRELLLAEEYP
MAAPKVRFMTKIYHPNVDKLERISLDILKDKWSPALQIRTVLLSIQALLN
APNPDDPLANDVVEQWKTNEAQAIETARAWTRLYAMNSI*

Vector Type

Mammalian

Antibiotic

Amp

Comments

N ter GST for mammalian expression. N.b. numerous differences at DNA level but agrees with UBE2NL protein sequence ref.

EStimated MW of expressed protein : 44635 Daltons

100042 R 1000 1001 1047 19007

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