USP17 | Ubiquitylation Resource

Plasmid

pcDNA5-FRT/TO-FLAG USP17

Parent Plasmid

pcDNA5D FRT/TO FLAG

Expressed

FLAG USP17

DU Number

DU12912

Genbank

NP_001099132

Tag

FLAG

Species

Human

Sequence of Insert

View Sequence

GGATCCATGGAGGACGACTCACTCTACTTGGGAGGTGAGTGGCAGTTCAA
CCACTTTCCAAAACTCACATCTTCTCGGCCCGATGCAGCTTTTGCTGAAA
TCCAGCGGACTTCTCTCCCTGAGAAGTCACCACTCTCATGTGAGACCCGT
GTCGACCTCTGTGATGATTTGGCTCCTGTGGCAAGACAGCTTGCTCCCAG
GGAGAAGCTTCCTCTGAGTAGCAGGAGACCTGCTGCGGTGGGGGCTGGGC
TCCAGAATATGGGAAATACCTGCTACGTGAACGCTTCCTTGGAGTGGCTG
ACATACACACCGCCCCTTGCCAACTACATGCTGTCCCGGGAGCACTCTCA
AACGTGTCATCGTCACAAGGGCTGCATGCTCTGTACTATGCAAGCTCACA
TCACACGGGCCCTCCACAATCCTGGCCACGTCATCCAGCCCTCACAGGCA
TTGGCTGCTGGCTTCCATAGAGGCAAGCAGGAAGATGCTCATGAATTTCT
CATGTTCACTGTGGATGCCATGAAAAAGGCATGCCTTCCCGGGCACAAGC
AGGTAGATCATCACTCTAAGGACACCACCCTCATCCACCAAATATTTGGA
GGCTACTGGAGATCTCAAATCAAGTGTCTCCACTGCCACGGCATTTCAGA
CACTTTTGACCCTTACCTGGACATCGCCCTGGATATCCAGGCAGCTCAGA
GTGTCCAGCAAGCTTTGGAACAGTTGGTGAAGCCCGAAGAACTCAATGGA
GAGAATGCCTATCATTGTGGTGTTTGTCTCCAGAGGGCGCCGGCCTCCAA
GACGTTAACTTTACACACCTCTGCCAAGGTCCTCATCCTTGTATTGAAGA
GATTCTCCGATGTCACAGGCAACAAGATTGCCAAGAATGTGCAATATCCT
GAGTGCCTTGACATGCAGCCATACATGTCTCAGCAGAACACAGGACCTCT
CGTCTATGTCCTCTATGCTGTGCTGGTCCACGCTGGGTGGAGTTGTCACA
ACGGACATTACTTCTCTTATGTCAAAGCTCAAGAAGGCCAGTGGTATAAA
ATGGATGATGCCGAGGTCACCGCCTCTAGCATCACTTCTGTCCTGAGTCA
ACAGGCCTACGTCCTCTTTTACATCCAGAAGAGTGAATGGGAAAGACACA
GTGAGAGTGTGTCAAGAGGCAGGGAACCAAGAGCCCTTGGCGCAGAAGAC
ACAGACAGGCGAGCAACGCAAGGAGAGCTCAAGAGAGACCACCCCTGCCT
CCAGGCCCCCGAGTTGGACGAGCACTTGGTGGAAAGAGCCACTCAGGAAA
GCACCTTAGACCACTGGAAATTCCTTCAAGAGCAAAACAAAACGAAGCCT
GAGTTCAACGTCAGAAAAGTCGAAGGTACCCTGCCTCCCGACGTACTTGT
GATTCATCAATCAAAATACAAGTGTGGGATGAAGAACCATCATCCTGAAC
AGCAAAGCTCCCTGCTAAACCTCTCTTCGACGACACCGACACATCAGGAG
TCCATGAACACTGGCACACTCGCTTCCCTGCGAGGGAGGGCCAGGAGATC
CAAAGGGAAGAACAAACACAGCAAGAGGGCTCTGCTTGTGTGCCAGTGAG
CGGCCGC

Amino Acid Sequence

View Sequence

MDYKDDDDKGSMEDDSLYLGGEWQFNHFPKLTSSRPDAAFAEIQRTSLPE
KSPLSCETRVDLCDDLAPVARQLAPREKLPLSSRRPAAVGAGLQNMGNTC
YVNASLEWLTYTPPLANYMLSREHSQTCHRHKGCMLCTMQAHITRALHNP
GHVIQPSQALAAGFHRGKQEDAHEFLMFTVDAMKKACLPGHKQVDHHSKD
TTLIHQIFGGYWRSQIKCLHCHGISDTFDPYLDIALDIQAAQSVQQALEQ
LVKPEELNGENAYHCGVCLQRAPASKTLTLHTSAKVLILVLKRFSDVTGN
KIAKNVQYPECLDMQPYMSQQNTGPLVYVLYAVLVHAGWSCHNGHYFSYV
KAQEGQWYKMDDAEVTASSITSVLSQQAYVLFYIQKSEWERHSESVSRGR
EPRALGAEDTDRRATQGELKRDHPCLQAPELDEHLVERATQESTLDHWKF
LQEQNKTKPEFNVRKVEGTLPPDVLVIHQSKYKCGMKNHHPEQQSSLLNL
SSTTPTHQESMNTGTLASLRGRARRSKGKNKHSKRALLVCQ*

Vector Type

Mammalian

Antibiotic

Amp

Comments

N ter FLAG for mammalian expression.

Estimated MW of expressed protein : 61018 Daltons

100042 R 1000 1001 1047 19007