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A20 (mouse)

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Plasmid
pCMV-FLAG-1 A20 (mouse)
Parent Plasmid
pCMV5 FLAG1
Expressed
FLAG A20
DU Number
DU32905
Genbank
AAH60221
Tag
FLAG
Species
Mouse
Sequence of Insert
GGATCCATGGCTGAACAACTTCTTCCTCAGGCTTTGTATTTGAGCAATAT
GCGGAAAGCTGTGAAGATACGAGAGAGAACCCCAGAAGACATTTTCAAAC
CTACCAATGGGATCATCTATCACTTTAAAACCATGCACCGATACACGCTG
GAGATGTTCAGAACATGCCAGTTTTGCCCACAGTTCCGAGAGATCATCCA
CAAAGCACTTATTGACAGAAGTGTCCAGGCTTCCCTGGAAAGCCAGAAGA
AGCTCAACTGGTGTCGTGAAGTCAGGAAGCTCGTGGCTCTGAAAACCAAT
GGTGATGGAAACTGCCTCATGCATGCAGCTTGTCAGTACATGTGGGGTGT
TCAGGATACTGACCTGGTCCTGAGGAAGGCCCTCTGCAGCACCCTTAAGG
AGACAGACACTCGGAACTTTAAATTCCGCTGGCAGCTGGAATCTCTGAAA
TCTCAGGAATTTGTGGAAACAGGACTTTGCTACGACACTCGGAACTGGAA
TGACGAATGGGACAACTTGGTCAAAATGGCATCAGCAGACACACCTGCAG
CCCGAAGTGGACTTCAGTACAATTCCCTGGAAGAAATCCACATATTTGTC
CTCAGCAACATCCTCAGAAGACCCATCATTGTCATTTCAGACAAAATGCT
AAGAAGTTTGGAATCTGGTTCCAATTTTGCTCCTTTGAAAGTGGGTGGGA
TTTATCTGCCTCTTCACTGGCCTGCCCAGGAGTGTTACAGATATCCCATC
GTCCTAGGCTATGACAGCCAGCACTTTGTACCCCTGGTGACCCTGAAGGA
CAGTGGACCTGAACTTCGCGCTGTTCCACTTGTTAACAGAGACCGGGGTA
GGTTTGAAGACTTAAAAGTTCACTTCTTGACAGATCCTGAGAATGAGATG
AAGGAAAAGCTTCTAAAGGAGTACTTGATAGTGATGGAGATCCCTGTGCA
AGGCTGGGACCACGGCACGACTCACCTGATCAACGCTGCAAAATTGGATG
AAGCTAACTTACCCAAAGAAATAAATTTGGTAGACGATTACTTTGAGCTT
GTTCAGCACGAATACAAGAAATGGCAGGAGAACAGCGATCAGGCCAGGAG
AGCGGCACATGCGCAGAACCCCTTGGAGCCTTCCACACCCCAGCTATCAC
TCATGGATATAAAATGTGAGACACCCAACTGTCCTTTCTTCATGTCCGTG
AACACTCAGCCTTTATTCCACGAATGCTCAGAGAGGCGCCAAAAGAATCA
GAGCAAGCTCCCAAAGCTGAACTCGAAGCTAGGCCCTGAAGGACTCCCAG
GCGTGGGACTTGGCTCCTCAAACTGGAGCCCCGAGGAAACCGCTGGAGGA
CCTCATTCAGCCCCACCCACAGCACCCAGCCTTTTTCTCTTCAGTGAGAC
CACTGCAATGAAGTGCAGGAGTCCTGGGTGCCCTTTTACTTTGAATGTGC
AGCATAATGGATTCTGTGAGCGTTGCCACGCCCGGCAGATTAATGCCAGC
CACACCGCAGACCCTGGAAAGTGCCAAGCCTGCCTTCAGGATGTCACTCG
GACCTTTAATGGCATCTGCAGTACCTGTTTCAAAAGGACTACAGCAGAGC
CCAGCTCCAGCCTCACTTCCAGTATCCCTGCCTCCTGTCACCAACGCTCC
AAGTCTGACCCCTCACAACTCATCCAAAGTCTCACTCCACACTCTTGCCA
CCGGACTGGAAATGTCTCTCCTTCTGGCTGCCTCTCCCAGGCTGCACGGA
CTCCAGGAGACAGAGCAGGGACAAGCAAGTGCAGGAAAGCTGGCTGCATG
TATTTTGGGACTCCAGAAAACAAGGGCTTTTGCACTCTATGTTTCATCGA
ATACAGAGAAAATAAGCAGTCTGTTACTGCCTCTGAGAAAGCTGGTTCCC
CGGCCCCCAGGTTCCAGAACAATGTCCCGTGCCTGGGCAGGGAGTGCGGC
ACACTCGGAAGCACCATGTTTGAAGGGTACTGTCAGAAGTGTTTCATCGA
AGCTCAGAACCAGAGATTCCATGAAGCAAGAAGAACGGAAGAACAGCTGA
GATCAAGCCAGCATAGAGACATGCCTCGAACTACACAGGTAGCCTCAAGG
CTGAAATGTGCCCGGGCCTCCTGCAAGAACATTCTGGCCTGTCGCAGTGA
GGAACTCTGTATGGAGTGCCAGCACCTAAGCCAACGAGTAGGTTCTGTGG
CCCACCGGGGTGAGCCCACGCCTGAAGAGCCCCCTAAACAGCGCTGCCGG
GCCCCTGCTTGTGATCACTTTGGCAATGCCAAGTGTAATGGTTACTGCAA
TGAGTGCTACCAGTTCAAGCAGATGTATGGCTAAGCGGCCGC
Amino Acid Sequence
MDYKDDDDKGSMAEQLLPQALYLSNMRKAVKIRERTPEDIFKPTNGIIYH
FKTMHRYTLEMFRTCQFCPQFREIIHKALIDRSVQASLESQKKLNWCREV
RKLVALKTNGDGNCLMHAACQYMWGVQDTDLVLRKALCSTLKETDTRNFK
FRWQLESLKSQEFVETGLCYDTRNWNDEWDNLVKMASADTPAARSGLQYN
SLEEIHIFVLSNILRRPIIVISDKMLRSLESGSNFAPLKVGGIYLPLHWP
AQECYRYPIVLGYDSQHFVPLVTLKDSGPELRAVPLVNRDRGRFEDLKVH
FLTDPENEMKEKLLKEYLIVMEIPVQGWDHGTTHLINAAKLDEANLPKEI
NLVDDYFELVQHEYKKWQENSDQARRAAHAQNPLEPSTPQLSLMDIKCET
PNCPFFMSVNTQPLFHECSERRQKNQSKLPKLNSKLGPEGLPGVGLGSSN
WSPEETAGGPHSAPPTAPSLFLFSETTAMKCRSPGCPFTLNVQHNGFCER
CHARQINASHTADPGKCQACLQDVTRTFNGICSTCFKRTTAEPSSSLTSS
IPASCHQRSKSDPSQLIQSLTPHSCHRTGNVSPSGCLSQAARTPGDRAGT
SKCRKAGCMYFGTPENKGFCTLCFIEYRENKQSVTASEKAGSPAPRFQNN
VPCLGRECGTLGSTMFEGYCQKCFIEAQNQRFHEARRTEEQLRSSQHRDM
PRTTQVASRLKCARASCKNILACRSEELCMECQHLSQRVGSVAHRGEPTP
EEPPKQRCRAPACDHFGNAKCNGYCNECYQFKQMYG*
Vector Type
Mammalian
Antibiotic
Amp
Comments
N terminal FLAG for mammalian expression

Estimated MW of expressed protein : 88910 Daltons
100042 R 1000 1001 1047 19007
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