USP34 | Ubiquitylation Resource

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Plasmid

pEBG6P-USP34 2561-3546

Parent Plasmid

pEBG6P

Expressed

GST USP34 2561-3546

DU Number

DU32517

Genbank

NM_014709.3

Tag

GST

Species

Human

Sequence of Insert

View Sequence

GGATCCATCAATATAAGACAAACTTGTAATCTGATTTTCAGCCTGTGTCG
ATACAATAATCGACTTGCAGAACATATTGTATCTATGCTTTTCACATCAA
TAGCAAAGTTGACTCCTGAGGCAGCCAATCCTTTCTTTAAGTTGTTGACT
ATGCTAATGGAGTTTGCTGGTGGACCTCCAGGAATGCCTCCCTTTGCATC
TTATATTCTGCAGAGGATATGGGAGGTGATTGAATACAATCCTTCTCAGT
GTCTAGATTGGTTGGCAGTGCAGACACCCCGAAATAAACTGGCACACAGC
TGGGTCTTACAGAATATGGAAAACTGGGTCGAGCGGTTTCTTTTGGCTCA
CAATTATCCTAGAGTGAGGACTTCTGCAGCTTATCTTCTGGTGTCCCTTA
TACCAAGCAATTCATTCCGTCAGATGTTCCGGTCAACAAGGTCTTTGCAC
ATCCCAACCCGTGACCTTCCACTCAGTCCAGACACAACAGTAGTCCTACA
TCAGGTCTACAACGTGCTCCTTGGTTTGCTCTCAAGAGCCAAACTTTATG
TTGATGCTGCTGTTCATGGCACTACAAAGCTAGTGCCCTATTTTAGCTTT
ATGACTTACTGTTTAATTTCCAAAACTGAGAAGCTGATGTTTTCCACATA
TTTCATGGATTTGTGGAACCTTTTCCAGCCTAAACTTTCTGAGCCAGCAA
TAGCTACAAATCACAATAAACAGGCTTTGCTTTCATTTTGGTACAATGTC
TGTGCTGACTGTCCAGAGAATATCCGCCTTATTGTTCAGAACCCAGTGGT
AACCAAGAACATTGCCTTCAATTACATCCTTGCTGACCATGATGATCAGG
ATGTGGTGCTTTTTAACCGTGGGATGCTGCCAGCGTACTATGGCATTCTG
AGGCTCTGCTGTGAGCAGTCTCCTGCATTCACACGACAACTGGCTTCTCA
CCAGAACATCCAGTGGGCCTTTAAGAATCTTACACCACATGCCAGCCAAT
ACCCTGGAGCAGTAGAAGAACTGTTTAACCTGATGCAGCTGTTTATAGCT
CAGAGGCCAGATATGAGAGAAGAAGAATTAGAAGATATTAAACAGTTCAA
GAAAACAACCATAAGTTGTTACTTACGTTGCTTAGATGGCCGCTCCTGCT
GGACTACTTTAATAAGTGCCTTCAGAATACTATTAGAATCTGATGAAGAC
AGACTTCTTGTTGTATTTAATCGAGGATTGATTCTAATGACAGAGTCTTT
CAACACTTTGCACATGATGTATCACGAAGCTACAGCTTGCCATGTGACTG
GAGATTTAGTAGAACTTCTGTCAATATTTCTTTCGGTTTTGAAGTCTACA
CGCCCTTATCTTCAGAGAAAAGATGTGAAACAAGCATTAATCCAGTGGCA
GGAGCGAATTGAATTTGCCCATAAACTGTTAACTCTTCTTAATTCCTATA
GTCCTCCAGAACTTAGAAATGCCTGTATAGATGTCCTCAAGGAACTTGTA
CTTTTGAGTCCCCATGATTTTCTTCATACTCTGGTTCCCTTTCTACAACA
CAACCATTGTACTTACCATCACAGTAATATACCAATGTCTCTTGGACCTT
ATTTCCCTTGTCGAGAAAATATCAAGCTAATAGGAGGGAAAAGCAATATT
CGGCCTCCGCGCCCTGAACTCAATATGTGCCTCTTGCCCACAATGGTGGA
AACCAGTAAGGGCAAAGATGACGTTTATGATCGTATGCTGCTAGACTACT
TCTTTTCTTATCATCAGTTCATCCATCTATTATGCCGAGTTGCAATCAAC
TGTGAAAAATTTACTGAAACATTAGTTAAGCTGAGTGTCCTAGTTGCCTA
TGAAGGTTTGCCACTTCATCTTGCACTGTTCCCCAAACTTTGGACTGAGC
TATGCCAGACTCAGTCTGCTATGTCAAAAAACTGCATCAAGCTTTTGTGT
GAAGATCCTGTTTTCGCAGAATATATTAAATGTATCCTAATGGATGAAAG
AACTTTTTTAAACAACAACATTGTCTACACGTTCATGACACATTTCCTTC
TAAAGGTTCAAAGTCAAGTGTTTTCTGAAGCAAACTGTGCCAATTTGATC
AGCACTCTTATTACAAACTTGATAAGCCAGTATCAGAACCTACAGTCTGA
TTTCTCCAACCGAGTTGAAATTTCCAAAGCAAGTGCTTCTTTAAATGGGG
ACCTGAGGGCACTCGCTTTGCTCCTGTCAGTACACACTCCCAAACAGTTA
AACCCAGCTCTAATTCCAACTCTGCAAGAGCTTTTAAGCAAATGCAGGAC
TTGTCTGCAACAGAGAAACTCACTCCAAGAGCAAGAAGCCAAAGAAAGAA
AAACTAAAGATGATGAAGGAGCAACTCCCATTAAAAGGCGGCGTGTTAGC
AGTGATGAGGAGCACACTGTAGACAGCTGCATCAGTGACATGAAAACAGA
AACCAGGGAGGTCCTGACCCCAACGAGCACTTCTGACAATGAGACCAGAG
ACTCCTCAATTATTGATCCAGGAACTGAGCAAGATCTTCCTTCCCCTGAA
AATAGTTCTGTTAAAGAATACCGAATGGAAGTTCCATCTTCGTTTTCAGA
AGACATGTCAAATATCAGGTCACAGCATGCAGAAGAACAGTCCAACAATG
GTAGATATGACGATTGTAAAGAATTTAAAGACCTCCACTGTTCCAAGGAT
TCTACCCTAGCTGAGGAAGAATCTGAGTTCCCTTCTACTTCTATCTCTGC
AGTTCTGTCTGACTTAGCTGACTTGAGAAGCTGTGATGGCCAAGCTTTGC
CCTCCCAGGACCCTGAGGTTGCTTTATCTCTCAGTTGTGGCCATTCCAGA
GGACTCTTTAGTCATATGCAGCAACATGACATTTTAGATACCCTGTGTAG
GACCATTGAATCTACAATCCATGTCGTCACAAGGATATCTGGCAAAGGAA
ACCAAGCTGCTTCTTGAGCGGCCGC

Amino Acid Sequence

View Sequence

MAPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSINIRQTCNLIFSL
CRYNNRLAEHIVSMLFTSIAKLTPEAANPFFKLLTMLMEFAGGPPGMPPF
ASYILQRIWEVIEYNPSQCLDWLAVQTPRNKLAHSWVLQNMENWVERFLL
AHNYPRVRTSAAYLLVSLIPSNSFRQMFRSTRSLHIPTRDLPLSPDTTVV
LHQVYNVLLGLLSRAKLYVDAAVHGTTKLVPYFSFMTYCLISKTEKLMFS
TYFMDLWNLFQPKLSEPAIATNHNKQALLSFWYNVCADCPENIRLIVQNP
VVTKNIAFNYILADHDDQDVVLFNRGMLPAYYGILRLCCEQSPAFTRQLA
SHQNIQWAFKNLTPHASQYPGAVEELFNLMQLFIAQRPDMREEELEDIKQ
FKKTTISCYLRCLDGRSCWTTLISAFRILLESDEDRLLVVFNRGLILMTE
SFNTLHMMYHEATACHVTGDLVELLSIFLSVLKSTRPYLQRKDVKQALIQ
WQERIEFAHKLLTLLNSYSPPELRNACIDVLKELVLLSPHDFLHTLVPFL
QHNHCTYHHSNIPMSLGPYFPCRENIKLIGGKSNIRPPRPELNMCLLPTM
VETSKGKDDVYDRMLLDYFFSYHQFIHLLCRVAINCEKFTETLVKLSVLV
AYEGLPLHLALFPKLWTELCQTQSAMSKNCIKLLCEDPVFAEYIKCILMD
ERTFLNNNIVYTFMTHFLLKVQSQVFSEANCANLISTLITNLISQYQNLQ
SDFSNRVEISKASASLNGDLRALALLLSVHTPKQLNPALIPTLQELLSKC
RTCLQQRNSLQEQEAKERKTKDDEGATPIKRRRVSSDEEHTVDSCISDMK
TETREVLTPTSTSDNETRDSSIIDPGTEQDLPSPENSSVKEYRMEVPSSF
SEDMSNIRSQHAEEQSNNGRYDDCKEFKDLHCSKDSTLAEEESEFPSTSI
SAVLSDLADLRSCDGQALPSQDPEVALSLSCGHSRGLFSHMQQHDILDTL
CRTIESTIHVVTRISGKGNQAAS*

Vector Type

Mammalian

Antibiotic

Amp

Comments

N ter GST for mammalian expression

100042 R 1000 1001 1047 19007

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