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UBE20

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Plasmid
pET156P UBE20
Parent Plasmid
pET15b 6His 3C
Expressed
6His UBE20
DU Number
DU32152
Genbank
AAH51868
Tag
6His
Species
Human
Sequence of Insert
GGATCCATGACCTCAGCCGACGTGATGTGGCAGGATGGCTCCGTGGAATG
CAACATCCGCTCCAACGACCTCTTCCCTGTGCACCACCTGGACAACAACG
AGTTCTGCCCTGGAGACTTCGTGGTAGATAAGCGAGTCCAGAGCTGTCCA
GACCCTGCTGTCTACGGTGTGGTACAGTCTGGGGACCACATCGGCCGTAC
CTGCATGGTGAAGTGGTTCAAGCTGAGGCCGAGTGGGGACGACGTGGAGC
TGATTGGAGAAGAGGAAGATGTGAGTGTTTACGACATTGCTGACCACCCT
GACTTTAGGTTCCGTACAACTGACATCGTCATCCGCATCGGCAATACTGA
GGATGGGGCTCCTCACAAGGAGGATGAGCCATCGGTGGGCCAGGTGGCCC
GTGTGGACGTCAGCAGCAAGGTGGAGGTGGTGTGGGCTGACAACTCAAAG
ACCATCATCCTGCCCCAGCACTTGTACAACATAGAGTCTGAGATTGAGGA
GTCAGACTACGATTCGGTAGAAGGCAGCACCAGCGGGGCATCCTCGGATG
AATGGGAAGATGATAGTGACAGCTGGGAGACGGACAATGGGCTGGTGGAG
GACGAGCACCCCAAGATAGAGGAGCCCCCCATCCCACCCCTGGAGCAGCC
GGTGGCCCCTGAGGACAAGGGAGTGGTGATCAGTGAAGAGGCAGCCACAG
CTGCCGTCCAGGGGGCTGTGGCCATGGCTGCCCCCATGGCCGGGCTGATG
GAGAAGGCTGGCAAGGACGGGCCACCCAAGAGCTTCCGGGAGTTGAAAGA
GGCCATCAAGATCCTGGAGAGCCTCAAGAACATGACTGTGGAGCAGCTGC
TGACGGGCTCGCCCACCTCTCCGACTGTGGAGCCTGAGAAGCCAACTCGG
GAGAAGAAGTTTCTGGATGACATCAAGAAGCTACAGGAAAACCTCAAGAA
GACCCTGGACAATGTGGCCATTGTAGAGGAGGAGAAGATGGAAGCAGTGC
CCGACGTAGAGCGCAAGGAGGACAAGCCCGAGGGGCAGTCACCTGTGAAG
GCTGAGTGGCCCAGCGAAACCCCGGTGCTGTGCCAGCAGTGTGGCGGCAA
GCCTGGCGTCACCTTCACCAGCGCCAAGGGCGAGGTCTTCTCCGTACTGG
AGTTTGCACCCTCAAATCATTCTTTTAAGAAAATTGAGTTCCAGCCTCCA
GAAGCCAAGAAGTTCTTCAGCACAGTGCGGAAGGAGATGGCGCTGCTGGC
TACCTCACTGCCTGAGGGCATCATGGTCAAGACTTTTGAAGATAGAATGG
ACCTCTTCTCAGCTCTCATCAAGGGCCCCACTCGAACCCCCTACGAGGAT
GGCCTCTACTTGTTTGACATCCAGCTCCCCAACATCTACCCAGCCGTGCC
CCCCCACTTCTGCTACCTCTCCCAATGCAGTGGCCGCCTGAACCCCAACC
TGTATGACAATGGGAAGGTGTGTGTCAGCCTCCTGGGCACCTGGATTGGA
AAGGGGACAGAGAGGTGGACAAGCAAGTCCAGCCTTCTCCAGGTGCTCAT
CTCCATCCAAGGTCTGATCCTGGTAAATGAACCATACTACAACGAAGCCG
GCTTCGACAGTGACCGAGGCCTGCAGGAAGGCTATGAAAACAGTCGCTGT
TACAATGAGATGGCGCTGATCCGCGTGGTGCAGTCCATGACCCAGCTGGT
GCGGCGGCCCCCCGAGGTCTTTGAGCAGGAGATCAGGCAACACTTTAGCA
CTGGTGGCTGGCGGCTGGTGAACCGTATCGAGTCCTGGCTGGAAACCCAT
GCCCTGCTGGAGAAGGCCCAGGCACTGCCCAACGGGGTGCCCAAGGCCAG
CAGCTCGCCAGAGCCCCCAGCTGTAGCCGAGCTGTCAGACTCCGGCCAAC
AAGAACCTGAGGATGGAGGGCCAGCCCCAGGAGAGGCCTCCCAGGGCTCA
GACTCAGAGGGCGGTGCCCAGGGCCTGGCCTCAGCTAGCAGGGACCACAC
AGACCAGACTTCGGAGACCGCACCAGACGCATCGGTGCCACCCAGTGTGA
AACCAAAGAAGCGGAGAAAGAGCTACCGGAGCTTCTTACCTGAGAAGAGT
GGCTACCCTGACATCGGCTTCCCCCTCTTCCCACTTTCCAAGGGTTTCAT
CAAGAGCATCCGGGGTGTCCTGACGCAGTTCCGGGCTGCCCTGCTAGAGG
CAGGCATGCCGGAGTGCACAGAGGACAAGTAGGCGGCCGC
Amino Acid Sequence
MGSSHHHHHHSSGLEVLFQGPGSMTSADVMWQDGSVECNIRSNDLFPVHH
LDNNEFCPGDFVVDKRVQSCPDPAVYGVVQSGDHIGRTCMVKWFKLRPSG
DDVELIGEEEDVSVYDIADHPDFRFRTTDIVIRIGNTEDGAPHKEDEPSV
GQVARVDVSSKVEVVWADNSKTIILPQHLYNIESEIEESDYDSVEGSTSG
ASSDEWEDDSDSWETDNGLVEDEHPKIEEPPIPPLEQPVAPEDKGVVISE
EAATAAVQGAVAMAAPMAGLMEKAGKDGPPKSFRELKEAIKILESLKNMT
VEQLLTGSPTSPTVEPEKPTREKKFLDDIKKLQENLKKTLDNVAIVEEEK
MEAVPDVERKEDKPEGQSPVKAEWPSETPVLCQQCGGKPGVTFTSAKGEV
FSVLEFAPSNHSFKKIEFQPPEAKKFFSTVRKEMALLATSLPEGIMVKTF
EDRMDLFSALIKGPTRTPYEDGLYLFDIQLPNIYPAVPPHFCYLSQCSGR
LNPNLYDNGKVCVSLLGTWIGKGTERWTSKSSLLQVLISIQGLILVNEPY
YNEAGFDSDRGLQEGYENSRCYNEMALIRVVQSMTQLVRRPPEVFEQEIR
QHFSTGGWRLVNRIESWLETHALLEKAQALPNGVPKASSSPEPPAVAELS
DSGQQEPEDGGPAPGEASQGSDSEGGAQGLASASRDHTDQTSETAPDASV
PPSVKPKKRRKSYRSFLPEKSGYPDIGFPLFPLSKGFIKSIRGVLTQFRA
ALLEAGMPECTEDK
Vector Type
Bacterial
Antibiotic
Amp
Comments
N ter 6HIS for bacterial expression

Estimated MW of expressed : 84160 Daltons
100042 R 1000 1001 1047 19007
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