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Plasmid
pGEX6P-1-Ubiquitin K29wt
Parent Plasmid
pGEX6P1
Expressed
GST-Ubiquitin K29wt
DU Number
DU8612
Genbank
P62988
Tag
GST
Species
Human
Sequence of Insert
View Sequence
GGATCCCAAATCTTCGTGAGGACCCTGACTGGTAGGACCATCACTCTCGA
AGTGGAGCCGAGTGACACCATTGAGAATGTCAGGGCAAAGATCCAAGACA
GGGAAGGCATCCCTCCTGACCAGCAGAGGTTGATCTTTGCTGGGAGACAG
CTGGAAGATGGACGCACCCTGTCTGACTACCACATCCAGAGAGAGTCCAC
CCTGCACCTGGTCCTCCGTCTCTTAGGTGGGTGATAACTCGAG
AGTGGAGCCGAGTGACACCATTGAGAATGTCAGGGCAAAGATCCAAGACA
GGGAAGGCATCCCTCCTGACCAGCAGAGGTTGATCTTTGCTGGGAGACAG
CTGGAAGATGGACGCACCCTGTCTGACTACCACATCCAGAGAGAGTCCAC
CCTGCACCTGGTCCTCCGTCTCTTAGGTGGGTGATAACTCGAG
Amino Acid Sequence
View Sequence
MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSQIFVRTLTGRTITLEVEPS
DTIENVRAKIQDREGIPPDQQRLIFAGRQLEDGRTLSDYHIQRESTLHLV
LRLLGG*
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSQIFVRTLTGRTITLEVEPS
DTIENVRAKIQDREGIPPDQQRLIFAGRQLEDGRTLSDYHIQRESTLHLV
LRLLGG*
Vector Type
Bacterial
Antibiotic
Amp
Comments
N terminal GST for bacterial expression.
100042 R 1000 1001 1047 19007
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