USP16 | Ubiquitylation Resource

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Plasmid

pET156P_1 USP16 Q141H E479D

Parent Plasmid

pET15b 6His 3C

Expressed

6His USP16 Q141H E479D

DU Number

DU15657

Genbank

AAH30777.1

Tag

6His

Species

Human

Sequence of Insert

View Sequence

GGATCCATGGGAAAGAAACGGACAAAGGGAAAAACTGTTCCAATCGATGA
TTCCTCTGAAACTTTAGAACCTGTGTGCAGACACATTAGAAAAGGATTGG
AACAAGGTAATTTGAAAAAGGCTTTAGTGAATGTGGAATGGAATATCTGC
CAAGACTGTAAGACTGACAATAAAGTGAAAGATAAAGCTGAAGAAGAAAC
AGAAGAAAAGCCTTCAGTTTGGCTGTGTCTTAAATGTGGCCATCAGGGCT
GTGGCAGAAATTCTCAGGAGCAGCATGCCTTGAAGCACTATCTGACGCCA
AGATCTGAACCTCACTGTCTGGTTCTTAGTTTGGACAACTGGAGTGTATG
GTGTTACGTATGTGATAATGAGGTCCAGTATTGTAGTTCAAACCAGTTGG
GTCAAGTGGTTGATTATGTCAGAAAACATGCCAGCATTACAACTCCAAAG
CCAGAGAAAGATAATGGAAATATTGAACTTGAAAATAAAAAATTAGAAAA
AGAGAGTAAGAATGAACAAGAGAGAGAAAAGAAGGAAAACATGGCTAAAG
AGAATCCTCCCATGAATTCTCCTTGCCAAATAACCGTGAAAGGACTCAGT
AATTTGGGAAACACATGTTTCTTCAATGCAGTTATGCAGAACTTGTCACA
AACACCAGTGCTTAGAGAACTACTAAAAGAAGTGAAAATGTCTGGAACAA
TTGTAAAAATTGAACCACCTGATTTGGCATTAACAGAACCATTAGAAATA
AACCTTGAGCCTCCAGGCCCTCTTACTTTAGCCATGAGCCAGTTTCTTAA
TGAGATGCAAGAGACCAAAAAGGGGGTTGTGACACCGAAAGAACTCTTTT
CTCAGGTCTGTAAAAAAGCAGTGCGGTTTAAAGGCTATCAGCAGCAAGAC
AGCCAGGAGCTGCTTCGCTACTTATTGGATGGGATGAGAGCAGAAGAACA
CCAAAGAGTGAGTAAAGGAATACTTAAAGCATTTGGTAATTCTACTGAAA
AGTTGGATGAAGAACTAAAAAATAAAGTTAAAGATTATGAGAAGAAAAAA
TCAATGCCAAGTTTTGTTGACCGCATCTTTGGTGGTGAACTAACTAGTAT
GATCATGTGTGATCAATGCAGAACTGTCTCCTTGGTTCATGAATCTTTCC
TTGATTTGTCCCTCCCAGTTTTAGATGATCAGAGTGGTAAGAAAAGTGTA
AATGATAAAAATCTGAAAAAGACAGTGGAGGATGAAGATCAAGATAGTGA
GGAAGAAAAAGATAACGACAGTTACATAAAAGAGAGAAGTGATATTCCTT
CTGGAACAAGTAAGCACTTACAGAAAAAAGCAAAGAAACAAGCCAAAAAG
CAAGCCAAGAACCAACGAAGACAACAAAAAATTCAAGGAAAAGTTCTTCA
TTTAAATGATATTTGTACTATTGACCATCCTGAAGACAGTGATAATGAAG
CTGAAATGTCACTTCAAGGAGAAGTAAATATTAAATCCAACCATATTTCA
CAAGAGGGTGTTATGCATAAAGAATATTGTGTCAACCAGAAAGATTTGAA
TGGCCAAGCAAAAATGATCGAAAGTGTAACTGACAATCAAAAATCCACAG
AGGAAGTAGATATGAAAAATATCAACATGGATAATGATCTGGAGGTTTTA
ACATCTTCTCCCACTAGGAATTTAAATGGTGCCTACCTAACGGAAGGGAG
CAATGGAGAAGTGGACATTTCCAATGGTTTCAAAAACCTAAATTTGAATG
CTGCTCTTCATCCTGATGAAATAAATATAGAGATTCTGAATGATAGTCAT
ACTCCTGGAACAAAGGTGTATGAGGTTGTAAATGAAGATCCAGAAACTGC
TTTCTGTACTCTTGCAAACAGGGAAGTTTTCAATACTGATGAGTGTTCAA
TCCAACATTGTTTATATCAGTTCACCCGTAATGAGAAACTTCGAGATGCG
AATAAACTGCTTTGTGAAGTATGCACACGGAGACAGTGTAATGGACCAAA
GGCAAATATAAAAGGTGAAAGGAAGCATGTTTACACCAATGCCAAAAAGC
AGATGCTAATTTCTCTTGCTCCTCCTGTTCTTACTCTTCATTTAAAGAGA
TTTCAGCAGGCTGGTTTTAACCTACGCAAAGTTAACAAACACATAAAGTT
TCCGGAAATCTTAGATTTGGCTCCTTTTTGCACCCTTAAATGTAAGAATG
TTGCAGAAGAAAATACAAGGGTACTCTATTCCTTATATGGAGTTGTTGAA
CACAGTGGTACTATGAGGTCGGGGCATTACACTGCCTATGCCAAGGCAAG
AACCGCAAATAGTCATCTCTCTAATCTTGTTCTTCACGGTGATATTCCAC
AAGATTTTGAAATGGAATCAAAAGGGCAGTGGTTTCACATCAGCGACACA
CATGTGCAAGCTGTGCCTACAACTAAAGTACTAAACTCACAAGCGTACCT
CCTATTTTATGAGAGAATACTGTAAGCGGCCGC

Amino Acid Sequence

View Sequence

MGSSHHHHHHSSGLEVLFQGPGSMGKKRTKGKTVPIDDSSETLEPVCRHI
RKGLEQGNLKKALVNVEWNICQDCKTDNKVKDKAEEETEEKPSVWLCLKC
GHQGCGRNSQEQHALKHYLTPRSEPHCLVLSLDNWSVWCYVCDNEVQYCS
SNQLGQVVDYVRKHASITTPKPEKDNGNIELENKKLEKESKNEQEREKKE
NMAKENPPMNSPCQITVKGLSNLGNTCFFNAVMQNLSQTPVLRELLKEVK
MSGTIVKIEPPDLALTEPLEINLEPPGPLTLAMSQFLNEMQETKKGVVTP
KELFSQVCKKAVRFKGYQQQDSQELLRYLLDGMRAEEHQRVSKGILKAFG
NSTEKLDEELKNKVKDYEKKKSMPSFVDRIFGGELTSMIMCDQCRTVSLV
HESFLDLSLPVLDDQSGKKSVNDKNLKKTVEDEDQDSEEEKDNDSYIKER
SDIPSGTSKHLQKKAKKQAKKQAKNQRRQQKIQGKVLHLNDICTIDHPED
SDNEAEMSLQGEVNIKSNHISQEGVMHKEYCVNQKDLNGQAKMIESVTDN
QKSTEEVDMKNINMDNDLEVLTSSPTRNLNGAYLTEGSNGEVDISNGFKN
LNLNAALHPDEINIEILNDSHTPGTKVYEVVNEDPETAFCTLANREVFNT
DECSIQHCLYQFTRNEKLRDANKLLCEVCTRRQCNGPKANIKGERKHVYT
NAKKQMLISLAPPVLTLHLKRFQQAGFNLRKVNKHIKFPEILDLAPFCTL
KCKNVAEENTRVLYSLYGVVEHSGTMRSGHYTAYAKARTANSHLSNLVLH
GDIPQDFEMESKGQWFHISDTHVQAVPTTKVLNSQAYLLFYERIL*

Vector Type

Bacterial

Antibiotic

Kan

Comments

N ter 6HIS for bacterial expression.

Estimated MW of expressed protein : 95887 Daltons

100042 R 1000 1001 1047 19007

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