USP7

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Plasmid
pCMV-FLAG USP7 1-550
Parent Plasmid
pCMV5 FLAG1
Expressed
FLAG USP7 1-550
DU Number
DU32684
Genbank
NP_003461.2
Tag
FLAG
Species
Human
Sequence of Insert
GGATCCATGAACCACCAGCAGCAGCAGCAGCAGCAGAAAGCGGGCGAGCA
GCAGTTGAGCGAGCCCGAGGACATGGAGATGGAAGCGGGAGATACAGATG
ACCCACCAAGAATTACTCAGAACCCTGTGATCAATGGGAATGTGGCCCTG
AGTGATGGACACAACACCGCGGAGGAGGACATGGAGGATGACACCAGTTG
GCGCTCCGAGGCAACCTTTCAGTTCACTGTGGAGCGCTTCAGCAGACTGA
GTGAGTCGGTCCTTAGCCCTCCGTGTTTTGTGCGAAATCTGCCATGGAAG
ATTATGGTGATGCCACGCTTTTATCCAGACAGACCACACCAAAAAAGCGT
AGGATTCTTTCTCCAGTGCAATGCTGAATCTGATTCCACGTCATGGTCTT
GCCATGCACAAGCAGTGCTGAAGATAATAAATTACAGAGATGATGAAAAG
TCGTTCAGTCGTCGTATTAGTCATTTGTTCTTCCATAAAGAAAATGATTG
GGGATTTTCCAATTTTATGGCCTGGAGTGAAGTGACCGATCCTGAGAAAG
GATTTATAGATGATGACAAAGTTACCTTTGAAGTCTTTGTACAGGCGGAT
GCTCCCCATGGAGTTGCGTGGGATTCAAAGAAGCACACAGGCTACGTCGG
CTTAAAGAATCAGGGAGCGACTTGTTACATGAACAGCCTGCTACAGACGT
TATTTTTCACGAATCAGCTACGAAAGGCTGTGTACATGATGCCAACCGAG
GGGGATGATTCGTCTAAAAGCGTCCCTTTAGCATTACAAAGAGTGTTCTA
TGAATTACAGCATAGTGATAAACCTGTAGGAACAAAAAAGTTAACAAAGT
CATTTGGGTGGGAAACTTTAGATAGCTTCATGCAACATGATGTTCAGGAG
CTTTGTCGAGTGTTGCTCGATAATGTGGAAAATAAGATGAAAGGCACCTG
TGTAGAGGGCACCATACCCAAATTATTCCGCGGCAAAATGGTGTCCTATA
TCCAGTGTAAAGAAGTAGACTATCGGTCTGATAGAAGAGAAGATTATTAT
GATATCCAGCTAAGTATCAAAGGAAAGAAAAATATATTTGAATCATTTGT
GGATTATGTGGCAGTAGAACAGCTCGATGGGGACAATAAATACGACGCTG
GGGAACATGGCTTACAGGAAGCAGAGAAAGGTGTGAAATTCCTAACATTG
CCACCAGTGTTACATCTACAACTGATGAGATTTATGTATGACCCTCAGAC
GGACCAAAATATCAAGATCAATGATAGGTTTGAATTCCCAGAGCAGTTAC
CACTTGATGAATTTTTGCAAAAAACAGATCCTAAGGACCCTGCAAATTAT
ATTCTTCATGCAGTCCTGGTTCATAGTGGAGATAATCATGGTGGACATTA
TGTGGTTTATCTAAACCCCAAAGGGGATGGCAAATGGTGTAAATTTGATG
ACGACGTGGTGTCAAGGTGTACTAAAGAGGAAGCAATTGAGCACAATTAT
GGGGGTCACGATGACGACCTGTCTGTTCGACACTGCACTAATGCTTACAT
GTTAGTCTACATCAGGGAATCAAAACTGAGTGAAGTTTTACAGGCGGTCA
CCGACCATGATATTCCTCAGCAGTTGGTGGAGCGATTACAAGAAGAGAAA
AGGATCTAGGCTCAGAAGCGGAAGGAGCGGCAGGAAGCCCATCTCTATAT
GCAAGTGCAGATAGTCGCAGAGGACCAGTTTTGTGGCCACCAAGGGAATG
ACATGTACGATGAAGAAAAAGTGAAATACACTGTGTTCAAAGTATTGAAG
AACTCCTCGCTTGCTGAGTTTGTTCAGAGCCTCTCTCAGACCATGGGATT
TCCACAAGATCAAATTCGATTGTGGCCCATGCAAGCAAGGAGTAATGGAA
CAAAACGACCAGCAATGTTAGATAATGAAGCCGACGGCAATAAAACAATG
ATTGAGCTCAGTGATAATGAAAACCCTTGGACAATATTCCTGGAAACAGT
TGATCCCGAGCTGGCTGCTAGTGGAGCGACCTTACCCAAGTTTGATAAAG
ATCATGATGTAATGTTATTTTTGAAGATGTATGATCCCAAAACGCGGAGC
TTGAATTACTGTGGGCATATCTACACACCAATATCCTGTAAAATACGTGA
CTTGCTCCCAGTTATGTGTGACAGAGCAGGATTTATTCAAGATACTAGCC
TTATCCTCTATGAGGAAGTTAAACCGAATTTAACAGAGAGAATTCAGGAC
TATGACGTGTCTCTTGATAAAGCCCTTGATGAACTAATGGATGGTGACAT
CATAGTATTTCAGAAGGATGACCCTGAAAATGATAACAGTGAATTACCCA
CCGCAAAGGAGTATTTCCGAGATCTCTACCACCGCGTTGATGTCATTTTC
TGTGATAAAACAATCCCTAATGATCCTGGATTTGTGGTTACGTTATCAAA
TAGAATGAATTATTTTCAGGTTGCAAAGACAGTTGCACAGAGGCTCAACA
CAGATCCAATGTTGCTGCAGTTTTTCAAGTCTCAAGGTTATAGGGATGGC
CCAGGTAATCCTCTTAGACATAATTATGAAGGTACTTTAAGAGATCTTCT
ACAGTTCTTCAAGCCTAGACAACCTAAGAAACTTTACTATCAGCAGCTTA
AGATGAAAATCACAGACTTTGAGAACAGGCGAAGTTTTAAATGTATATGG
TTAAACAGCCAATTTAGGGAAGAGGAAATAACACTATATCCAGACAAGCA
TGGGTGTGTCCGGGACCTGTTAGAAGAATGTAAAAAGGCCGTGGAGCTTG
GGGAGAAAGCATCAGGGAAACTTAGGCTGCTAGAAATTGTAAGCTACAAA
ATCATTGGTGTTCATCAAGAAGATGAACTATTAGAATGTTTATCTCCTGC
AACGAGCCGGACGTTTCGAATAGAGGAAATCCCTTTGGACCAGGTGGACA
TAGACAAAGAGAATGAGATGCTTGTCACAGTGGCGCATTTCCACAAAGAG
GTCTTCGGAACGTTCGGAATCCCGTTTTTGCTGAGGATACACCAGGGCGA
GCATTTTCGAGAAGTGATGAAGCGAATCCAGAGCCTGCTGGACATCCAGG
AGAAGGAGTTTGAGAAGTTTAAATTTGCAATTGTAATGATGGGCCGACAC
CAGTACATAAATGAAGACGAGTATGAAGTAAATTTGAAAGACTTTGAGCC
ACAGCCCGGTAATATGTCTCATCCTCGGCCTTGGCTAGGGCTCGACCACT
TCAACAAAGCCCCAAAGAGGAGTCGCTACACTTACCTTGAAAAGGCCATT
AAAATCCATAACTGAGCGGCCGC
Amino Acid Sequence
MDYKDDDDKGSMNHQQQQQQQKAGEQQLSEPEDMEMEAGDTDDPPRITQN
PVINGNVALSDGHNTAEEDMEDDTSWRSEATFQFTVERFSRLSESVLSPP
CFVRNLPWKIMVMPRFYPDRPHQKSVGFFLQCNAESDSTSWSCHAQAVLK
IINYRDDEKSFSRRISHLFFHKENDWGFSNFMAWSEVTDPEKGFIDDDKV
TFEVFVQADAPHGVAWDSKKHTGYVGLKNQGATCYMNSLLQTLFFTNQLR
KAVYMMPTEGDDSSKSVPLALQRVFYELQHSDKPVGTKKLTKSFGWETLD
SFMQHDVQELCRVLLDNVENKMKGTCVEGTIPKLFRGKMVSYIQCKEVDY
RSDRREDYYDIQLSIKGKKNIFESFVDYVAVEQLDGDNKYDAGEHGLQEA
EKGVKFLTLPPVLHLQLMRFMYDPQTDQNIKINDRFEFPEQLPLDEFLQK
TDPKDPANYILHAVLVHSGDNHGGHYVVYLNPKGDGKWCKFDDDVVSRCT
KEEAIEHNYGGHDDDLSVRHCTNAYMLVYIRESKLSEVLQAVTDHDIPQQ
LVERLQEEKRI*
Vector Type
Mammalian
Antibiotic
Amp
Comments
N ter FLAG for mammalian expression

Estimated MW of expressed protein : 64826 Daltons
100042 R 1000 1001 1047 19007
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