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Plasmid
pCMV-FLAG-1 NEDD4
Parent Plasmid
pCMV5 FLAG1
Expressed
FLAG NEDD4
DU Number
DU32250
Genbank
NM_006154.2
Tag
FLAG
Species
Human
Sequence of Insert
View Sequence
GGATCCATGGCAACTTGCGCGGTGGAGGTGTTCGGGCTCCTGGAGGACGA
GGAAAATTCACGAATTGTGAGAGTAAGAGTTATAGCCGGAATAGGCCTTG
CCAAGAAGGATATATTGGGAGCTAGTGATCCTTACGTGAGAGTGACGTTA
TATGACCCAATGAATGGAGTTCTTACAAGTGTGCAAACAAAAACCATTAA
AAAGAGTTTGAATCCAAAGTGGAATGAAGAAATATTATTCAGAGTTCATC
CTCAGCAGCACCGGCTTCTTTTTGAAGTGTTTGACGAAAACCGATTGACA
AGAGATGATTTCCTAGGTCAAGTGGATGTTCCACTTTATCCATTACCGAC
AGAAAATCCAAGATTGGAGAGACCATATACATTTAAGGATTTTGTTCTTC
ATCCAAGAAGTCACAAATCAAGAGTTAAAGGTTATCTGAGACTAAAAATG
ACTTATTTACCTAAAACCAGTGGCTCAGAAGATGATAATGCAGAACAGGC
TGAGGAATTAGAGCCTGGCTGGGTTGTTTTGGACCAACCAGATGCTGCTT
GCCATTTGCAGCAACAACAAGAACCTTCTCCTCTACCTCCAGGGTGGGAA
GAGAGGCAGGATATCCTTGGAAGGACCTATTATGTAAACCATGAATCTAG
AAGAACACAGTGGAAAAGACCAACCCCTCAGGACAACCTAACAGATGCTG
AGAATGGCAACATTCAACTGCAAGCACAACGTGCATTTACCACCAGGCGG
CAGATATCCGAGGAAACAGAAAGTGTTGACAACCAAGAGTCTTCCGAGAA
CTGGGAAATTATAAGAGAAGATGAAGCCACCATGTATAGCAGCCAGGCCT
TCCCATCACCTCCACCGTCAAGTAACTTGGATGTTCCAACTCATCTTGCA
GAAGAATTGAATGCCAGACTCACCATTTTTGGAAATTCAGCCGTGAGCCA
GCCAGCATCGAGCTCAAATCATTCCAGCAGAAGAGGCAGCTTACAAGCCT
ATACTTTTGAGGAACAACCTACACTTCCTGTGCTTTTGCCTACTTCATCT
GGATTACCACCAGGTTGGGAAGAAAAACAAGATGAAAGAGGAAGATCATA
TTATGTAGATCACAATTCCAGAACGACTACTTGGACAAAGCCCACTGTAC
AGGCCACAGTGGAGACCAGTCAGCTGACCTCAAGCCAGAGTTCTGCAGGC
CCTCAATCACAAGCCTCCACCAGTGATTCAGGCCAGCAGGTGACCCAGCC
ATCTGAAATTGAGCAAGGATTCCTTCCTAAAGGCTGGGAAGTCCGGCATG
CACCAAATGGGAGGCCTTTCTTTATTGACCACAACACTAAAACCACCACC
TGGGAAGATCCAAGATTGAAAATTCCAGCCCATCTGAGAGGAAAGACATC
ACTTGATACTTCCAATGATCTAGGGCCTTTACCTCCAGGATGGGAAGAGA
GAACTCACACAGATGGAAGAATCTTCTACATAAATCACAATATAAAAAGA
ACACAATGGGAAGATCCTCGGTTGGAGAATGTAGCAATAACTGGACCAGC
AGTGCCCTACTCCAGGGATTACAAAAGAAAGTATGAGTTCTTCCGAAGAA
AGTTGAAGAAGCAGAATGACATTCCAAACAAATTTGAAATGAAACTTCGC
CGAGCAACTGTTCTTGAAGACTCTTACCGGAGAATTATGGGTGTCAAGAG
AGCAGACTTCCTGAAGGCTCGACTGTGGATTGAGTTTGATGGTGAAAAGG
GATTGGATTATGGAGGAGTTGCCAGAGAATGGTTCTTCCTGATCTCAAAG
GAAATGTTTAACCCTTATTATGGGTTGTTTGAATATTCTGCTACGGACAA
TTATACCCTACAGATAAATCCAAACTCTGGATTGTGTAACGAAGATCACC
TCTCTTACTTCAAGTTTATTGGTCGGGTAGCTGGAATGGCAGTTTATCAT
GGCAAACTGTTGGATGGTTTTTTCATCCGCCCATTTTACAAGATGATGCT
TCACAAACCAATAACCCTTCATGATATGGAATCTGTGGATAGTGAATATT
ACAATTCCCTAAGATGGATTCTTGAAAATGACCCAACAGAATTGGACCTC
AGGTTTATCATAGATGAAGAACTTTTTGGACAGACACATCAACATGAGCT
GAAAAATGGTGGATCAGAAATAGTTGTCACCAATAAGAACAAAAAGGAAT
ATATTTATCTTGTAATACAATGGCGATTTGTAAACCGAATCCAGAAGCAA
ATGGCTGCTTTTAAAGAGGGATTCTTTGAACTAATACCACAGGATCTCAT
CAAAATTTTTGATGAAAATGAACTAGAGCTTCTTATGTGTGGACTGGGAG
ATGTTGATGTGAATGACTGGAGGGAACATACAAAGTATAAAAATGGCTAC
AGTGCAAATCATCAGGTTATACAGTGGTTTTGGAAGGCTGTTTTAATGAT
GGATTCAGAAAAAAGAATAAGATTACTTCAGTTTGTCACTGGCACATCTC
GGGTGCCTATGAATGGATTTGCTGAACTATACGGTTCAAATGGACCACAG
TCATTTACAGTTGAACAGTGGGGTACGCCTGAAAAGCTGCCAAGAGCTCA
TACCTGTTTTAATCGCCTGGACTTGCCACCTTATGAATCATTTGAAGAAT
TATGGGATAAACTTCAGATGGCAATTGAAAACACCCAGGGCTTTGATGGA
GTTGATTAGGCGGCCGC
GGAAAATTCACGAATTGTGAGAGTAAGAGTTATAGCCGGAATAGGCCTTG
CCAAGAAGGATATATTGGGAGCTAGTGATCCTTACGTGAGAGTGACGTTA
TATGACCCAATGAATGGAGTTCTTACAAGTGTGCAAACAAAAACCATTAA
AAAGAGTTTGAATCCAAAGTGGAATGAAGAAATATTATTCAGAGTTCATC
CTCAGCAGCACCGGCTTCTTTTTGAAGTGTTTGACGAAAACCGATTGACA
AGAGATGATTTCCTAGGTCAAGTGGATGTTCCACTTTATCCATTACCGAC
AGAAAATCCAAGATTGGAGAGACCATATACATTTAAGGATTTTGTTCTTC
ATCCAAGAAGTCACAAATCAAGAGTTAAAGGTTATCTGAGACTAAAAATG
ACTTATTTACCTAAAACCAGTGGCTCAGAAGATGATAATGCAGAACAGGC
TGAGGAATTAGAGCCTGGCTGGGTTGTTTTGGACCAACCAGATGCTGCTT
GCCATTTGCAGCAACAACAAGAACCTTCTCCTCTACCTCCAGGGTGGGAA
GAGAGGCAGGATATCCTTGGAAGGACCTATTATGTAAACCATGAATCTAG
AAGAACACAGTGGAAAAGACCAACCCCTCAGGACAACCTAACAGATGCTG
AGAATGGCAACATTCAACTGCAAGCACAACGTGCATTTACCACCAGGCGG
CAGATATCCGAGGAAACAGAAAGTGTTGACAACCAAGAGTCTTCCGAGAA
CTGGGAAATTATAAGAGAAGATGAAGCCACCATGTATAGCAGCCAGGCCT
TCCCATCACCTCCACCGTCAAGTAACTTGGATGTTCCAACTCATCTTGCA
GAAGAATTGAATGCCAGACTCACCATTTTTGGAAATTCAGCCGTGAGCCA
GCCAGCATCGAGCTCAAATCATTCCAGCAGAAGAGGCAGCTTACAAGCCT
ATACTTTTGAGGAACAACCTACACTTCCTGTGCTTTTGCCTACTTCATCT
GGATTACCACCAGGTTGGGAAGAAAAACAAGATGAAAGAGGAAGATCATA
TTATGTAGATCACAATTCCAGAACGACTACTTGGACAAAGCCCACTGTAC
AGGCCACAGTGGAGACCAGTCAGCTGACCTCAAGCCAGAGTTCTGCAGGC
CCTCAATCACAAGCCTCCACCAGTGATTCAGGCCAGCAGGTGACCCAGCC
ATCTGAAATTGAGCAAGGATTCCTTCCTAAAGGCTGGGAAGTCCGGCATG
CACCAAATGGGAGGCCTTTCTTTATTGACCACAACACTAAAACCACCACC
TGGGAAGATCCAAGATTGAAAATTCCAGCCCATCTGAGAGGAAAGACATC
ACTTGATACTTCCAATGATCTAGGGCCTTTACCTCCAGGATGGGAAGAGA
GAACTCACACAGATGGAAGAATCTTCTACATAAATCACAATATAAAAAGA
ACACAATGGGAAGATCCTCGGTTGGAGAATGTAGCAATAACTGGACCAGC
AGTGCCCTACTCCAGGGATTACAAAAGAAAGTATGAGTTCTTCCGAAGAA
AGTTGAAGAAGCAGAATGACATTCCAAACAAATTTGAAATGAAACTTCGC
CGAGCAACTGTTCTTGAAGACTCTTACCGGAGAATTATGGGTGTCAAGAG
AGCAGACTTCCTGAAGGCTCGACTGTGGATTGAGTTTGATGGTGAAAAGG
GATTGGATTATGGAGGAGTTGCCAGAGAATGGTTCTTCCTGATCTCAAAG
GAAATGTTTAACCCTTATTATGGGTTGTTTGAATATTCTGCTACGGACAA
TTATACCCTACAGATAAATCCAAACTCTGGATTGTGTAACGAAGATCACC
TCTCTTACTTCAAGTTTATTGGTCGGGTAGCTGGAATGGCAGTTTATCAT
GGCAAACTGTTGGATGGTTTTTTCATCCGCCCATTTTACAAGATGATGCT
TCACAAACCAATAACCCTTCATGATATGGAATCTGTGGATAGTGAATATT
ACAATTCCCTAAGATGGATTCTTGAAAATGACCCAACAGAATTGGACCTC
AGGTTTATCATAGATGAAGAACTTTTTGGACAGACACATCAACATGAGCT
GAAAAATGGTGGATCAGAAATAGTTGTCACCAATAAGAACAAAAAGGAAT
ATATTTATCTTGTAATACAATGGCGATTTGTAAACCGAATCCAGAAGCAA
ATGGCTGCTTTTAAAGAGGGATTCTTTGAACTAATACCACAGGATCTCAT
CAAAATTTTTGATGAAAATGAACTAGAGCTTCTTATGTGTGGACTGGGAG
ATGTTGATGTGAATGACTGGAGGGAACATACAAAGTATAAAAATGGCTAC
AGTGCAAATCATCAGGTTATACAGTGGTTTTGGAAGGCTGTTTTAATGAT
GGATTCAGAAAAAAGAATAAGATTACTTCAGTTTGTCACTGGCACATCTC
GGGTGCCTATGAATGGATTTGCTGAACTATACGGTTCAAATGGACCACAG
TCATTTACAGTTGAACAGTGGGGTACGCCTGAAAAGCTGCCAAGAGCTCA
TACCTGTTTTAATCGCCTGGACTTGCCACCTTATGAATCATTTGAAGAAT
TATGGGATAAACTTCAGATGGCAATTGAAAACACCCAGGGCTTTGATGGA
GTTGATTAGGCGGCCGC
Amino Acid Sequence
View Sequence
MDYKDDDDKGSMATCAVEVFGLLEDEENSRIVRVRVIAGIGLAKKDILGA
SDPYVRVTLYDPMNGVLTSVQTKTIKKSLNPKWNEEILFRVHPQQHRLLF
EVFDENRLTRDDFLGQVDVPLYPLPTENPRLERPYTFKDFVLHPRSHKSR
VKGYLRLKMTYLPKTSGSEDDNAEQAEELEPGWVVLDQPDAACHLQQQQE
PSPLPPGWEERQDILGRTYYVNHESRRTQWKRPTPQDNLTDAENGNIQLQ
AQRAFTTRRQISEETESVDNQESSENWEIIREDEATMYSSQAFPSPPPSS
NLDVPTHLAEELNARLTIFGNSAVSQPASSSNHSSRRGSLQAYTFEEQPT
LPVLLPTSSGLPPGWEEKQDERGRSYYVDHNSRTTTWTKPTVQATVETSQ
LTSSQSSAGPQSQASTSDSGQQVTQPSEIEQGFLPKGWEVRHAPNGRPFF
IDHNTKTTTWEDPRLKIPAHLRGKTSLDTSNDLGPLPPGWEERTHTDGRI
FYINHNIKRTQWEDPRLENVAITGPAVPYSRDYKRKYEFFRRKLKKQNDI
PNKFEMKLRRATVLEDSYRRIMGVKRADFLKARLWIEFDGEKGLDYGGVA
REWFFLISKEMFNPYYGLFEYSATDNYTLQINPNSGLCNEDHLSYFKFIG
RVAGMAVYHGKLLDGFFIRPFYKMMLHKPITLHDMESVDSEYYNSLRWIL
ENDPTELDLRFIIDEELFGQTHQHELKNGGSEIVVTNKNKKEYIYLVIQW
RFVNRIQKQMAAFKEGFFELIPQDLIKIFDENELELLMCGLGDVDVNDWR
EHTKYKNGYSANHQVIQWFWKAVLMMDSEKRIRLLQFVTGTSRVPMNGFA
ELYGSNGPQSFTVEQWGTPEKLPRAHTCFNRLDLPPYESFEELWDKLQMA
IENTQGFDGVD
SDPYVRVTLYDPMNGVLTSVQTKTIKKSLNPKWNEEILFRVHPQQHRLLF
EVFDENRLTRDDFLGQVDVPLYPLPTENPRLERPYTFKDFVLHPRSHKSR
VKGYLRLKMTYLPKTSGSEDDNAEQAEELEPGWVVLDQPDAACHLQQQQE
PSPLPPGWEERQDILGRTYYVNHESRRTQWKRPTPQDNLTDAENGNIQLQ
AQRAFTTRRQISEETESVDNQESSENWEIIREDEATMYSSQAFPSPPPSS
NLDVPTHLAEELNARLTIFGNSAVSQPASSSNHSSRRGSLQAYTFEEQPT
LPVLLPTSSGLPPGWEEKQDERGRSYYVDHNSRTTTWTKPTVQATVETSQ
LTSSQSSAGPQSQASTSDSGQQVTQPSEIEQGFLPKGWEVRHAPNGRPFF
IDHNTKTTTWEDPRLKIPAHLRGKTSLDTSNDLGPLPPGWEERTHTDGRI
FYINHNIKRTQWEDPRLENVAITGPAVPYSRDYKRKYEFFRRKLKKQNDI
PNKFEMKLRRATVLEDSYRRIMGVKRADFLKARLWIEFDGEKGLDYGGVA
REWFFLISKEMFNPYYGLFEYSATDNYTLQINPNSGLCNEDHLSYFKFIG
RVAGMAVYHGKLLDGFFIRPFYKMMLHKPITLHDMESVDSEYYNSLRWIL
ENDPTELDLRFIIDEELFGQTHQHELKNGGSEIVVTNKNKKEYIYLVIQW
RFVNRIQKQMAAFKEGFFELIPQDLIKIFDENELELLMCGLGDVDVNDWR
EHTKYKNGYSANHQVIQWFWKAVLMMDSEKRIRLLQFVTGTSRVPMNGFA
ELYGSNGPQSFTVEQWGTPEKLPRAHTCFNRLDLPPYESFEELWDKLQMA
IENTQGFDGVD
Vector Type
Mammalian
Antibiotic
Amp
100042 R 1000 1001 1047 19007
Download datasheet
Estimated MW of expressed protein : 105429 Daltons