Ubiquitin | Ubiquitylation Resource

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Plasmid

pHisTEV30a Ubiquitin M-2C K48E

Parent Plasmid

pHisTEV30a

Expressed

6His TEV Ubiquitin M-2C K48E

DU Number

DU61209

Genbank

N/A

Tag

6His

Species

Human

Sequence of Insert

View Sequence

CATATGTCGTACTACCATCACCATCACCATCACGATTACGATATCCCAACGACCGAAAACCTGTATTTTCAGGGCGCCTGCGGTATGCAGATCTTCGTGAAGACCCTGACTGGTAAGACCATCACTCTCGAAGTGGAGCCGAGTGACACCATTGAGAATGTCAAGGCAAAGATCCAAGACAAGGAAGGCATCCCTCCTGACCAGCAGAGGTTGATCTTTGCTGGGGAACAGCTGGAAGATGGACGCACCCTGTCTGACTACAACATCCAGAAAGAGTCCACCCTGCACCTGGTCCTCCGTCTCCGAGGTGGGTGATAAAAGCTTGCGGCCGC

Amino Acid Sequence

View Sequence

MSYYHHHHHHDYDIPTTENLYFQGACGMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGEQLEDGRTLSDYNIQKESTLHLVLRL
RGG*

Vector Type

Bacterial

Antibiotic

Kan

Comments

Compared to NCBI reference sequence there i a silent mutation at R73 (a220c).
Clone contains Cys residue two amino acids upstream of Ubiquitin start - introduced for labelling of ubiquitin with fluorescent dye.

100042 R 1000 1001 1047 19007

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