This reagent and more can be found at our sister site MRC PPU Reagents & Services 
Request Now
Plasmid
pGEX TEV UBE3A C843A
Parent Plasmid
pGEX TEV
Expressed
GST-TEV-UBE3A C843A
DU Number
DU65473
Genbank
Uniprot: Q05086, NCBI: NM_000462.3
Tag
GST
Species
Human
Sequence of Insert
View Sequence
ggatccATGGAGAAGCTGCACCAGTGTTATTGGAAATCAGGAGAACCTCA
GTCTGACGACATTGAAGCTAGCCGAATGAAGCGAGCAGCTGCAAAGCATC
TAATAGAACGCTACTACCACCAGTTAACTGAGGGCTGTGGAAATGAAGCC
TGCACGAATGAGTTTTGTGCTTCCTGTCCAACTTTTCTTCGTATGGATAA
TAATGCAGCAGCTATTAAAGCCCTCGAGCTTTATAAGATTAATGCAAAAC
TCTGTGATCCTCATCCCTCCAAGAAAGGAGCAAGCTCAGCTTACCTTGAG
AACTCGAAAGGTGCCCCCAACAACTCCTGCTCTGAGATAAAAATGAACAA
GAAAGGCGCTAGAATTGATTTTAAAGATGTGACTTACTTAACAGAAGAGA
AGGTATATGAAATTCTTGAATTATGTAGAGAAAGAGAGGATTATTCCCCT
TTAATCCGTGTTATTGGAAGAGTTTTTTCTAGTGCTGAGGCATTGGTACA
GAGCTTCCGGAAAGTTAAACAACACACCAAGGAAGAACTGAAATCTCTTC
AAGCAAAAGATGAAGACAAAGATGAAGATGAAAAGGAAAAAGCTGCATGT
TCTGCTGCTGCTATGGAAGAAGACTCAGAAGCATCTTCCTCAAGGATAGG
TGATAGCTCACAGGGAGACAACAATTTGCAAAAATTAGGCCCTGATGATG
TGTCTGTGGATATTGATGCCATTAGAAGGGTCTACACCAGATTGCTCTCT
AATGAAAAAATTGAAACTGCCTTTCTCAATGCACTTGTATATTTGTCACC
TAACGTGGAATGTGACTTGACGTATCACAATGTATACTCTCGAGATCCTA
ATTATCTGAATTTGTTCATTATCGTAATGGAGAATAGAAATCTCCACAGT
CCTGAATATCTGGAAATGGCTTTGCCATTATTTTGCAAAGCGATGAGCAA
GCTACCCCTTGCAGCCCAAGGAAAACTGATCAGACTGTGGTCTAAATACA
ATGCAGACCAGATTCGGAGAATGATGGAGACATTTCAGCAACTTATTACT
TATAAAGTCATAAGCAATGAATTTAACAGTCGAAATCTAGTGAATGATGA
TGATGCCATTGTTGCTGCTTCGAAGTGCTTGAAAATGGTTTACTATGCAA
ATGTAGTGGGAGGGGAAGTGGACACAAATCACAATGAAGAAGATGATGAA
GAGCCCATCCCTGAGTCCAGCGAGCTGACACTTCAGGAACTTTTGGGAGA
AGAAAGAAGAAACAAGAAAGGTCCTCGAGTGGACCCCCTGGAAACTGAAC
TTGGTGTTAAAACCCTGGATTGTCGAAAACCACTTATCCCTTTTGAAGAG
TTTATTAATGAACCACTGAATGAGGTTCTAGAAATGGATAAAGATTATAC
TTTTTTCAAAGTAGAAACAGAGAACAAATTCTCTTTTATGACATGTCCCT
TTATATTGAATGCTGTCACAAAGAATTTGGGATTATATTATGACAATAGA
ATTCGCATGTACAGTGAACGAAGAATCACTGTTCTCTACAGCTTAGTTCA
AGGACAGCAGTTGAATCCATATTTGAGACTCAAAGTTAGACGTGACCATA
TCATAGATGATGCACTTGTCCGGCTAGAGATGATCGCTATGGAAAATCCT
GCAGACTTGAAGAAGCAGTTGTATGTGGAATTTGAAGGAGAACAAGGAGT
TGATGAGGGAGGTGTTTCCAAAGAATTTTTTCAGCTGGTTGTGGAGGAAA
TCTTCAATCCAGATATTGGTATGTTCACATACGATGAATCTACAAAATTG
TTTTGGTTTAATCCATCTTCTTTTGAAACTGAGGGTCAGTTTACTCTGAT
TGGCATAGTACTGGGTCTGGCTATTTACAATAACTGTATACTGGATGTAC
ATTTTCCCATGGTTGTCTACAGGAAGCTAATGGGGAAAAAAGGAACTTTT
CGTGACTTGGGAGACTCTCACCCAGTTCTATATCAGAGTTTAAAAGATTT
ATTGGAGTATGAAGGGAATGTGGAAGATGACATGATGATCACTTTCCAGA
TATCACAGACAGATCTTTTTGGTAACCCAATGATGTATGATCTAAAGGAA
AATGGTGATAAAATTCCAATTACAAATGAAAACAGGAAGGAATTTGTCAA
TCTTTATTCTGACTACATTCTCAATAAATCAGTAGAAAAACAGTTCAAGG
CTTTTCGGAGAGGTTTTCATATGGTGACCAATGAATCTCCCTTAAAGTAC
TTATTCAGACCAGAAGAAATTGAATTGCTTATATGTGGAAGCCGGAATCT
AGATTTCCAAGCACTAGAAGAAACTACAGAATATGACGGTGGCTATACCA
GGGACTCTGTTCTGATTAGGGAGTTCTGGGAAATCGTTCATTCATTTACA
GATGAACAGAAAAGACTCTTCTTGCAGTTTACAACGGGCACAGACAGAGC
ACCTGTGGGAGGACTAGGAAAATTAAAGATGATTATAGCCAAAAATGGCC
CAGACACAGAAAGGTTACCTACATCTCATACTgcCTTTAATGTGCTTTTA
CTTCCGGAATACTCAAGCAAAGAAAAACTTAAAGAGAGATTGTTGAAGGC
CATCACGTATGCCAAAGGATTTGGCATGCTGTAAgcggccgc
GTCTGACGACATTGAAGCTAGCCGAATGAAGCGAGCAGCTGCAAAGCATC
TAATAGAACGCTACTACCACCAGTTAACTGAGGGCTGTGGAAATGAAGCC
TGCACGAATGAGTTTTGTGCTTCCTGTCCAACTTTTCTTCGTATGGATAA
TAATGCAGCAGCTATTAAAGCCCTCGAGCTTTATAAGATTAATGCAAAAC
TCTGTGATCCTCATCCCTCCAAGAAAGGAGCAAGCTCAGCTTACCTTGAG
AACTCGAAAGGTGCCCCCAACAACTCCTGCTCTGAGATAAAAATGAACAA
GAAAGGCGCTAGAATTGATTTTAAAGATGTGACTTACTTAACAGAAGAGA
AGGTATATGAAATTCTTGAATTATGTAGAGAAAGAGAGGATTATTCCCCT
TTAATCCGTGTTATTGGAAGAGTTTTTTCTAGTGCTGAGGCATTGGTACA
GAGCTTCCGGAAAGTTAAACAACACACCAAGGAAGAACTGAAATCTCTTC
AAGCAAAAGATGAAGACAAAGATGAAGATGAAAAGGAAAAAGCTGCATGT
TCTGCTGCTGCTATGGAAGAAGACTCAGAAGCATCTTCCTCAAGGATAGG
TGATAGCTCACAGGGAGACAACAATTTGCAAAAATTAGGCCCTGATGATG
TGTCTGTGGATATTGATGCCATTAGAAGGGTCTACACCAGATTGCTCTCT
AATGAAAAAATTGAAACTGCCTTTCTCAATGCACTTGTATATTTGTCACC
TAACGTGGAATGTGACTTGACGTATCACAATGTATACTCTCGAGATCCTA
ATTATCTGAATTTGTTCATTATCGTAATGGAGAATAGAAATCTCCACAGT
CCTGAATATCTGGAAATGGCTTTGCCATTATTTTGCAAAGCGATGAGCAA
GCTACCCCTTGCAGCCCAAGGAAAACTGATCAGACTGTGGTCTAAATACA
ATGCAGACCAGATTCGGAGAATGATGGAGACATTTCAGCAACTTATTACT
TATAAAGTCATAAGCAATGAATTTAACAGTCGAAATCTAGTGAATGATGA
TGATGCCATTGTTGCTGCTTCGAAGTGCTTGAAAATGGTTTACTATGCAA
ATGTAGTGGGAGGGGAAGTGGACACAAATCACAATGAAGAAGATGATGAA
GAGCCCATCCCTGAGTCCAGCGAGCTGACACTTCAGGAACTTTTGGGAGA
AGAAAGAAGAAACAAGAAAGGTCCTCGAGTGGACCCCCTGGAAACTGAAC
TTGGTGTTAAAACCCTGGATTGTCGAAAACCACTTATCCCTTTTGAAGAG
TTTATTAATGAACCACTGAATGAGGTTCTAGAAATGGATAAAGATTATAC
TTTTTTCAAAGTAGAAACAGAGAACAAATTCTCTTTTATGACATGTCCCT
TTATATTGAATGCTGTCACAAAGAATTTGGGATTATATTATGACAATAGA
ATTCGCATGTACAGTGAACGAAGAATCACTGTTCTCTACAGCTTAGTTCA
AGGACAGCAGTTGAATCCATATTTGAGACTCAAAGTTAGACGTGACCATA
TCATAGATGATGCACTTGTCCGGCTAGAGATGATCGCTATGGAAAATCCT
GCAGACTTGAAGAAGCAGTTGTATGTGGAATTTGAAGGAGAACAAGGAGT
TGATGAGGGAGGTGTTTCCAAAGAATTTTTTCAGCTGGTTGTGGAGGAAA
TCTTCAATCCAGATATTGGTATGTTCACATACGATGAATCTACAAAATTG
TTTTGGTTTAATCCATCTTCTTTTGAAACTGAGGGTCAGTTTACTCTGAT
TGGCATAGTACTGGGTCTGGCTATTTACAATAACTGTATACTGGATGTAC
ATTTTCCCATGGTTGTCTACAGGAAGCTAATGGGGAAAAAAGGAACTTTT
CGTGACTTGGGAGACTCTCACCCAGTTCTATATCAGAGTTTAAAAGATTT
ATTGGAGTATGAAGGGAATGTGGAAGATGACATGATGATCACTTTCCAGA
TATCACAGACAGATCTTTTTGGTAACCCAATGATGTATGATCTAAAGGAA
AATGGTGATAAAATTCCAATTACAAATGAAAACAGGAAGGAATTTGTCAA
TCTTTATTCTGACTACATTCTCAATAAATCAGTAGAAAAACAGTTCAAGG
CTTTTCGGAGAGGTTTTCATATGGTGACCAATGAATCTCCCTTAAAGTAC
TTATTCAGACCAGAAGAAATTGAATTGCTTATATGTGGAAGCCGGAATCT
AGATTTCCAAGCACTAGAAGAAACTACAGAATATGACGGTGGCTATACCA
GGGACTCTGTTCTGATTAGGGAGTTCTGGGAAATCGTTCATTCATTTACA
GATGAACAGAAAAGACTCTTCTTGCAGTTTACAACGGGCACAGACAGAGC
ACCTGTGGGAGGACTAGGAAAATTAAAGATGATTATAGCCAAAAATGGCC
CAGACACAGAAAGGTTACCTACATCTCATACTgcCTTTAATGTGCTTTTA
CTTCCGGAATACTCAAGCAAAGAAAAACTTAAAGAGAGATTGTTGAAGGC
CATCACGTATGCCAAAGGATTTGGCATGCTGTAAgcggccgc
Amino Acid Sequence
View Sequence
MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDENLYFQGGSMEKLHQCYWKSGEPQSDDIEA
SRMKRAAAKHLIERYYHQLTEGCGNEACTNEFCASCPTFLRMDNNAAAIK
ALELYKINAKLCDPHPSKKGASSAYLENSKGAPNNSCSEIKMNKKGARID
FKDVTYLTEEKVYEILELCREREDYSPLIRVIGRVFSSAEALVQSFRKVK
QHTKEELKSLQAKDEDKDEDEKEKAACSAAAMEEDSEASSSRIGDSSQGD
NNLQKLGPDDVSVDIDAIRRVYTRLLSNEKIETAFLNALVYLSPNVECDL
TYHNVYSRDPNYLNLFIIVMENRNLHSPEYLEMALPLFCKAMSKLPLAAQ
GKLIRLWSKYNADQIRRMMETFQQLITYKVISNEFNSRNLVNDDDAIVAA
SKCLKMVYYANVVGGEVDTNHNEEDDEEPIPESSELTLQELLGEERRNKK
GPRVDPLETELGVKTLDCRKPLIPFEEFINEPLNEVLEMDKDYTFFKVET
ENKFSFMTCPFILNAVTKNLGLYYDNRIRMYSERRITVLYSLVQGQQLNP
YLRLKVRRDHIIDDALVRLEMIAMENPADLKKQLYVEFEGEQGVDEGGVS
KEFFQLVVEEIFNPDIGMFTYDESTKLFWFNPSSFETEGQFTLIGIVLGL
AIYNNCILDVHFPMVVYRKLMGKKGTFRDLGDSHPVLYQSLKDLLEYEGN
VEDDMMITFQISQTDLFGNPMMYDLKENGDKIPITNENRKEFVNLYSDYI
LNKSVEKQFKAFRRGFHMVTNESPLKYLFRPEEIELLICGSRNLDFQALE
ETTEYDGGYTRDSVLIREFWEIVHSFTDEQKRLFLQFTTGTDRAPVGGLG
KLKMIIAKNGPDTERLPTSHTAFNVLLLPEYSSKEKLKERLLKAITYAKG
FGML*
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDENLYFQGGSMEKLHQCYWKSGEPQSDDIEA
SRMKRAAAKHLIERYYHQLTEGCGNEACTNEFCASCPTFLRMDNNAAAIK
ALELYKINAKLCDPHPSKKGASSAYLENSKGAPNNSCSEIKMNKKGARID
FKDVTYLTEEKVYEILELCREREDYSPLIRVIGRVFSSAEALVQSFRKVK
QHTKEELKSLQAKDEDKDEDEKEKAACSAAAMEEDSEASSSRIGDSSQGD
NNLQKLGPDDVSVDIDAIRRVYTRLLSNEKIETAFLNALVYLSPNVECDL
TYHNVYSRDPNYLNLFIIVMENRNLHSPEYLEMALPLFCKAMSKLPLAAQ
GKLIRLWSKYNADQIRRMMETFQQLITYKVISNEFNSRNLVNDDDAIVAA
SKCLKMVYYANVVGGEVDTNHNEEDDEEPIPESSELTLQELLGEERRNKK
GPRVDPLETELGVKTLDCRKPLIPFEEFINEPLNEVLEMDKDYTFFKVET
ENKFSFMTCPFILNAVTKNLGLYYDNRIRMYSERRITVLYSLVQGQQLNP
YLRLKVRRDHIIDDALVRLEMIAMENPADLKKQLYVEFEGEQGVDEGGVS
KEFFQLVVEEIFNPDIGMFTYDESTKLFWFNPSSFETEGQFTLIGIVLGL
AIYNNCILDVHFPMVVYRKLMGKKGTFRDLGDSHPVLYQSLKDLLEYEGN
VEDDMMITFQISQTDLFGNPMMYDLKENGDKIPITNENRKEFVNLYSDYI
LNKSVEKQFKAFRRGFHMVTNESPLKYLFRPEEIELLICGSRNLDFQALE
ETTEYDGGYTRDSVLIREFWEIVHSFTDEQKRLFLQFTTGTDRAPVGGLG
KLKMIIAKNGPDTERLPTSHTAFNVLLLPEYSSKEKLKERLLKAITYAKG
FGML*
Vector Type
Bacterial
Antibiotic
Amp
Comments
Transcript Variant 2
100042 R 1000 1001 1047 19007
Download datasheet