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Plasmid
pET15-UBIQUITIN-6HIS TAG72
Parent Plasmid
pET156P-1
Expressed
ubiquitin-His TAG72
DU Number
DU65366
Genbank
AAB25195.1
Tag
6His
Species
Human
Sequence of Insert
View Sequence
catATGCAGATCTTCGTGAAGACCCTGACTGGTAAGACCATCACTCTCGAAGTGGAGCCGAGTGACACCATTGAGAATGTCAAGGCAAAGATCCAAGACAAGGAAGGCATCCCTCCTGACCAGCAGAGGTTGATCTTTGCTGGGAAACAGCTGGAAGATGGACGCACCCTGTCTGACTACAACATCCAGAAAGAGTCCACCCTGCACCTGGTCCTCTAGCTCCGAGGTGGGCATCACCATCACCATCACTAAGCGGCCGC
Amino Acid Sequence
View Sequence
MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQL
EDGRTLSDYNIQKESTLHLVL*LRGGHHHHHH*
EDGRTLSDYNIQKESTLHLVL*LRGGHHHHHH*
Vector Type
Bacterial
Antibiotic
Amp
Comments
N-ter 6His deleted. C-terminal Hisx6 tag immediately follows GG (retain TAA stop codon). extra residues (MGS) upstream of ubiquitin Met1 removed from 29183
100042 R 1000 1001 1047 19007
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