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Plasmid
pTXB1 Ubiquitin (Met1-Leu73) TAG31 -T3C Mxe intein/chitin binding domain
Parent Plasmid
pTXB-1
Expressed
TAG31 Ubiquitin 1-73-T3C Mxe intein/chitin binding domain
DU Number
DU65282
Genbank
P62988
Tag
Intein
Species
Human
Sequence of Insert
View Sequence
CATATGCAGATCTTCGTCAAGACGTTAACCGGTAAAACCATAACTCTAGAAGTGGAACCGAGCGATACCATCGAAAACGTGAAAGCGAAAATCTAGGATAAAGAAGGCATCCCGCCGGATCAGCAGCGTCTGATCTTTGCGGGCAAACAGCTGGAAGATGGCCGCACCCTGTCTGATTATAACATCCAGAAAGAGTCGACCCTGCATCTGGTCTTAAGACTGTGCATCTGCGGAGATGCACTAGTTGCCCTACCCGAGGGCGAGTCGGTACGCATCGCCGACATCGTGCCGGGTGCGCGGCCCAACAGTGACAACGCCATCGACCTGAAAGTCCTTGACCGGCATGGCAATCCCGTGCTCGCCGACCGGCTGTTCCACTCCGGCGAGCATCCGGTGTACACGGTGCGTACGGTCGAAGGTCTGCGTGTGACGGGCACCGCGAACCACCCGTTGTTGTGTTTGGTCGACGTCGCCGGGGTGCCGACCCTGCTGTGGAAGCTGATCGACGAAATCAAGCCGGGCGATTACGCGGTGATTCAACGCAGCGCATTCAGCGTCGACTGTGCAGGTTTTGCCCGCGGGAAACCCGAATTTGCGCCCACAACCTACACAGTCGGCGTCCCTGGACTGGTGCGTTTCTTGGAAGCACACCACCGAGACCCGGACGCCCAAGCTATCGCCGACGAGCTGACCGACGGGCGGTTCTACTACGCGAAAGTCGCCAGTGTCACCGACGCCGGCGTGCAGCCGGTGTATAGCCTTCGTGTCGACACGGCAGACCACGCGTTTATCACGAACGGGTTCGTCAGCCACGCTACTGGCCTCACCGGTCTGAACTCAGGCCTCACGACAAATCCTGGTGTATCCGCTTGGCAGGTCAACACAGCTTATACTGCGGGACAATTGGTCACATATAACGGCAAGACGTATAAATGTTTGCAGCCCCACACCTCCTTGGCAGGATGGGAACCATCCAACGTTCCTGCCTTGTGGCAGCTTCAATGACTGCAG
Amino Acid Sequence
View Sequence
MQIFVKTLTGKTITLEVEPSDTIENVKAKI*DKEGIPPDQQRLIFAGKQL
EDGRTLSDYNIQKESTLHLVLRLCICGDALVALPEGESVRIADIVPGARP
NSDNAIDLKVLDRHGNPVLADRLFHSGEHPVYTVRTVEGLRVTGTANHPL
LCLVDVAGVPTLLWKLIDEIKPGDYAVIQRSAFSVDCAGFARGKPEFAPT
TYTVGVPGLVRFLEAHHRDPDAQAIADELTDGRFYYAKVASVTDAGVQPV
YSLRVDTADHAFITNGFVSHATGLTGLNSGLTTNPGVSAWQVNTAYTAGQ
LVTYNGKTYKCLQPHTSLAGWEPSNVPALWQLQ*
EDGRTLSDYNIQKESTLHLVLRLCICGDALVALPEGESVRIADIVPGARP
NSDNAIDLKVLDRHGNPVLADRLFHSGEHPVYTVRTVEGLRVTGTANHPL
LCLVDVAGVPTLLWKLIDEIKPGDYAVIQRSAFSVDCAGFARGKPEFAPT
TYTVGVPGLVRFLEAHHRDPDAQAIADELTDGRFYYAKVASVTDAGVQPV
YSLRVDTADHAFITNGFVSHATGLTGLNSGLTTNPGVSAWQVNTAYTAGQ
LVTYNGKTYKCLQPHTSLAGWEPSNVPALWQLQ*
Vector Type
Bacterial
Antibiotic
Amp
Comments
Ubiquitin sequence is codon-optimised. Obtained by Satpal Virdee from outwith MRCPPU. NOT FOR REDISTRIBUTION.
100042 R 1000 1001 1047 19007
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