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Plasmid
pET24-Ubiquitin Thr12Ser
Parent Plasmid
pET24a
Expressed
Ubiquitin [Thr12Ser]
DU Number
DU29928
Genbank
P62988
Species
Human
Sequence of Insert
View Sequence
CATATGCAGATCTTCGTGAAGACCCTGACTGGTAAGtCCATCACTCTCGA
AGTGGAGCCGAGTGACACCATTGAGAATGTCAAGGCAAAGATCCAAGACA
AGGAAGGCATCCCTCCTGACCAGCAGAGGTTGATCTTTGCTGGGAAACAG
CTGGAAGATGGACGCACCCTGTCTGACTACAACATCCAGAAAGAGTCCAC
CCTGCACCTGGTCCTCCGTCTCCGAGGTGGGTGATAAGGATCC
AGTGGAGCCGAGTGACACCATTGAGAATGTCAAGGCAAAGATCCAAGACA
AGGAAGGCATCCCTCCTGACCAGCAGAGGTTGATCTTTGCTGGGAAACAG
CTGGAAGATGGACGCACCCTGTCTGACTACAACATCCAGAAAGAGTCCAC
CCTGCACCTGGTCCTCCGTCTCCGAGGTGGGTGATAAGGATCC
Amino Acid Sequence
View Sequence
MQIFVKTLTGKSITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQL
EDGRTLSDYNIQKESTLHLVLRLRGG**
EDGRTLSDYNIQKESTLHLVLRLRGG**
Vector Type
Bacterial
Antibiotic
Kan
Comments
Bacterial expression construct for untagged ubiquitin (Arg73 codon AGA mutated to more common CGA) Differs from reference sequence in having silent mutation at Arg73 (a220c substitution conferred by reverse primer 54389). T7 tag in pET24a removed by subcloning into the Nde1 site, to give untagged protein.
100042 R 1000 1001 1047 19007
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