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Plasmid
pFBDM-8His-mMBP-3Ala-Traf6 50-end R88A F118A F122A
Parent Plasmid
pFBDM-8His-mMBP-3Ala
Expressed
8His-mMBP-3Ala-Traf6 50-end R88A F118A F122A
DU Number
DU29579
Tag
6His
MBP
TrxA
Species
Human
Amino Acid Sequence
View Sequence
MGHHHHHHHHKTEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHP
DKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPAAAFQDKLY
PFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELK
AKGKSALMFNLQEPYFTWPLIAADGGYAFKYAAGKYDIKDVGVDNAGAKA
GLTFLVDLIKNKHMNADTDYSIAEHAFNHGETAMTINGPWAWSNIDTSAV
NYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGL
EAVNKDKPLGAVALKSYEEELVKDPRVAATMENAQKGEIMPNIPQMSAFW
YAVRTAVINAASGRQTVDAALAAAQTNAAAMEEIQGYDVEFDPPLESKYE
CPICLMALREAVQTPCGHAFCKACIIKSIRDAGHKCPVDNEILLENQLAP
DNAAKREILSLMVKCPNEGCLHKMELRHLEDHQAHCEFALMDCPQCQRPF
QKFHINIHILKDCPRRQVSCDNCAASMAFEDKEIHDQNCPLANVICEYCN
TILIREQMPNHYDLDCPTAPIPCTFSTFGCHEKMQRNHLARHLQENTQSH
MRMLAQAVHSLSVIPDSGYISEVRNFQETIHQLEGRLVRQDHQIRELTAK
METQSMYVSELKRTIRTLEDKVAEIEAQQCNGIYIWKIGNFGMHLKCQEE
EKPVVIHSPGFYTGKPGYKLCMRLHLQLPTAQRCANYISLFVHTMQGEYD
SHLPWPFQGTIRLTILDQSEAPVRQNHEEIMDAKPELLAFQRPTIPRNPK
GFGYVTFMHLEALRQRTFIKDDTLLVRCEVSTRFDMGSLRREGFQPRSTD
AGV*
DKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPAAAFQDKLY
PFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELK
AKGKSALMFNLQEPYFTWPLIAADGGYAFKYAAGKYDIKDVGVDNAGAKA
GLTFLVDLIKNKHMNADTDYSIAEHAFNHGETAMTINGPWAWSNIDTSAV
NYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGL
EAVNKDKPLGAVALKSYEEELVKDPRVAATMENAQKGEIMPNIPQMSAFW
YAVRTAVINAASGRQTVDAALAAAQTNAAAMEEIQGYDVEFDPPLESKYE
CPICLMALREAVQTPCGHAFCKACIIKSIRDAGHKCPVDNEILLENQLAP
DNAAKREILSLMVKCPNEGCLHKMELRHLEDHQAHCEFALMDCPQCQRPF
QKFHINIHILKDCPRRQVSCDNCAASMAFEDKEIHDQNCPLANVICEYCN
TILIREQMPNHYDLDCPTAPIPCTFSTFGCHEKMQRNHLARHLQENTQSH
MRMLAQAVHSLSVIPDSGYISEVRNFQETIHQLEGRLVRQDHQIRELTAK
METQSMYVSELKRTIRTLEDKVAEIEAQQCNGIYIWKIGNFGMHLKCQEE
EKPVVIHSPGFYTGKPGYKLCMRLHLQLPTAQRCANYISLFVHTMQGEYD
SHLPWPFQGTIRLTILDQSEAPVRQNHEEIMDAKPELLAFQRPTIPRNPK
GFGYVTFMHLEALRQRTFIKDDTLLVRCEVSTRFDMGSLRREGFQPRSTD
AGV*
Vector Type
Baculovirus
Antibiotic
Amp
Comments
Parental 29541. BamHI cloning site deleted to make TRAF6 ORF flush with 3Ala
100042 R 1000 1001 1047 19007
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