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Plasmid
pET15-MGCSSG-Ubiquitin TAG9-6His
Parent Plasmid
pET156P-1
Expressed
MGCSSG-Ub TAG9-6His
DU Number
DU29247
Tag
6His
Species
Human
Sequence of Insert
GGATCCATGGGTTGCTCTTCCGGCCAGATCTTCGTGAAGACCCTGTAGGGTAAGACCATCACTCTCGAAGTGGAGCCGAGTGACACCATTGAGAATGTCAAGGCAAAGATCCAAGACAAGGAAGGCATCCCTCCTGACCAGCAGAGGTTGATCTTTGCTGGGAAACAGCTGGAAGATGGACGCACCCTGTCTGACTACAACATCCAGAAAGAGTCCACCCTGCACCTGGTCCTCCGTCTCCGAGGTGGGCATCACCATCACCATCACTAAGCGGCCGC
Amino Acid Sequence
MGSMGCSSGQIFVKTL*GKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIF
AGKQLEDGRTLSDYNIQKESTLHLVLRLRGGHHHHHH*
Vector Type
Bacterial
Antibiotic
Amp
Comments
GCSSG sequence inserted between Met1 and Gln2. Clone also contains 3 extra residues (MGS) upstream of ubiquitin Met1
N-ter 6His deleted. C-terminal Hisx6 tag immediately follows GG (retained TAA stop codon).
100042 R 1000 1001 1047 19007
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