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Plasmid
pMEX TEV HA Ufm1 [G83C]
Parent Plasmid
pMEX
Expressed
MBP-TEV-HA-Ufm1 [G83C]
DU Number
DU55462
Genbank
P61960
Tag
HA
MBP
Sequence of Insert
View Sequence
GGATCCGAAAACCTGTATTTTCAGGGCATGTACCCATACGATGTGCCAGATTACGCCATGTCGAAGGTTTCCTTTAAGATCACGCTGACGTCGGACCCACGGCTGCCGTACAAAGTACTCAGTGTTCCTGAAAGTACACCTTTCACAGCAGTCTTAAAGTTTGCAGCAGAAGAATTTAAAGTTCCTGCTGCAACAAGTGCAATTATTACCAATGATGGAATAGGAATAAATCCTGCACAGACTGCTGGAAATGTTTTTCTAAAACATGGTTCAGAACTGCGGATTATTCCTAGAGATCGTGTTTGCAGTTGTTAAGCGAAGGGCGAATTC
Amino Acid Sequence
View Sequence
MKIEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYEEELVKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQTNSSSNNNNNNNNNNLGDDDDKVPEFLEVLFQGPLGSENLYFQGMYPYDVPDYAMSKVSFKITLTSDPRLPYKVLSVPESTPFTAVLKFAAEEFKVPAATSAIITNDGIGINPAQTAGNVFLKHGSELRIIPRDRVCSC*
Vector Type
Bacterial
Antibiotic
Amp
Comments
The His-tagged protein (DU55407) is not soluble. Please try to sub clone into a MPB-vector and do away with the His-tag. Thanks
100042 R 1000 1001 1047 19007
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