UFC1 | Ubiquitylation Resource

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Synonyms

Plasmid

pET15 6His 3C UFC1 Y90C

Parent Plasmid

pET15b 6His 3C

Expressed

6His 3C UFC1 Y90C

DU Number

DU53290

Genbank

NM_016406.3 GI:156415999*

Tag

6His

Species

Human

Sequence of Insert

View Sequence

GGATCCATGGCGGATGAAGCCACGCGACGTGTTGTGTCTGAGATCCCGGT
GCTGAAGACTAACGCCGGACCCCGAGATCGTGAGTTGTGGGTGCAGCGAC
TGAAGGAGGAATATCAGTCCCTTATCCGGTATGTGGAGAACAACAAGAAT
GCTGACAACGATTGGTTCCGACTGGAGTCCAACAAGGAAGGAACTCGGTG
GTTTGGAAAATGCTGGTATATCCATGACCTCCTGAAATATGAGTTTGACA
TCGAGTTTGACATTCCTATCACATGTCCTACTACTGCCCCAGAAATTGCA
GTTCCTGAGCTGGATGGAAAGACAGCAAAGATGTACAGGGGTGGCAAAAT
ATGCCTGACGGATCATTTCAAACCTTTGTGGGCCAGGAATGTGCCCAAAT
TTGGACTAGCTCATCTCATGGCTCTGGGGCTGGGTCCATGGCTGGCAGTG
GAAATCCCTGATCTGATTCAGAAGGGCGTCATCCAACACAAAGAGAAATG
CAACCAATGAGCGGCCGC

Amino Acid Sequence

View Sequence

MGSSHHHHHHSSGLEVLFQGPGSMADEATRRVVSEIPVLKTNAGPRDRELWVQRLKEEYQSLIRYVENNKNADNDWFRLESNKEGTRWFGKCWYIHDLLK
YEFDIEFDIPITCPTTAPEIAVPELDGKTAKMYRGGKICLTDHFKPLWARNVPKFGLAHLMALGLGPWLAVEIPDLIQKGVIQHKEKCNQ*

Vector Type

Bacterial

Antibiotic

Amp

Comments

*Sequence difference compared with NM_016406.3 CDS: a269g (Y90C) (matches BC005187.1 GI:13528770) - renders protein insoluble
Bacterial construct to express GST N terminal fusion

Estimated MW of expressed protein : 46221 Daltons

100042 R 1000 1001 1047 19007

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