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Plasmid
pET24a Ubiquitin 77W
Parent Plasmid
pET24a
Expressed
Ubiquitin-Tryptophan
DU Number
DU51317
Species
Human
Sequence of Insert
View Sequence
CATATGCAGATCTTCGTGAAGACCCTGACTGGTAAGACCATCACTCTCGA
AGTGGAGCCGAGTGACACCATTGAGAATGTCAAGGCAAAGATCCAAGACA
AGGAAGGCATCCCTCCTGACCAGCAGAGGTTGATCTTTGCTGGGAAACAG
CTGGAAGATGGACGCACCCTGTCTGACTACAACATCCAGAAAGAGTCCAC
CCTGCACCTGGTCCTCCGTCTCCGAGGTGGGTGGTAAGGATCC
AGTGGAGCCGAGTGACACCATTGAGAATGTCAAGGCAAAGATCCAAGACA
AGGAAGGCATCCCTCCTGACCAGCAGAGGTTGATCTTTGCTGGGAAACAG
CTGGAAGATGGACGCACCCTGTCTGACTACAACATCCAGAAAGAGTCCAC
CCTGCACCTGGTCCTCCGTCTCCGAGGTGGGTGGTAAGGATCC
Amino Acid Sequence
View Sequence
MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQL
EDGRTLSDYNIQKESTLHLVLRLRGGW*
EDGRTLSDYNIQKESTLHLVLRLRGGW*
Vector Type
Bacterial
Antibiotic
Kan
Comments
Hi there, please a Tryptophan residue to our ubiquitin clone DU20027, to create the expression plasmid. This protein, if we can purify it, may be a good UCH-L1 substrate
100042 R 1000 1001 1047 19007
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