TRAF6 [L74H] (mouse) | Ubiquitylation Resource

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Plasmid

pRETROX tight puro Traf6 (mouse) L74H

Parent Plasmid

pRetroX tight puro

Expressed

TRAF6 [L74H] (mouse)

DU Number

DU51041

Species

Mouse

Sequence of Insert

View Sequence

GGATCCATGAGTCTCTTAAACTGTGAGAACAGCTGCGGGTCCAGCCAGTCGTCCAGTGACTGCTGCGCTGCCATGGCCGCCTCCTGCAGCGCTGCAGTGA
AAGATGACAGCGTGAGTGGCTCTGCCAGCACCGGGAACCTCTCCAGCTCCTTCATGGAGGAGATCCAGGGCTACGATGTGGAGTTTGACCCACCTCTGGA
GAGCAAGTATGAGTGTCCCATCTGCCATATGGCTTTACGGGAAGCAGTGCAAACACCATGTGGCCACAGGTTCTGCAAAGCCTGCATCATCAAATCCATA
AGGGATGCAGGGCACAAGTGCCCAGTTGACAATGAAATACTGCTGGAAAATCAACTGTTTCCCGACAATTTTGCAAAGCGAGAGATTCTTTCCCTGACGG
TAAAGTGCCCAAATAAAGGCTGTTTGCAAAAGATGGAACTGAGACATCTCGAGGATCATCAAGTACATTGTGAATTTGCTCTAGTGAATTGTCCCCAGTG
CCAACGTCCTTTCCAGAAGTGCCAGGTTAATACACACATTATTGAGGATTGTCCCAGGAGGCAGGTTTCTTGTGTAAACTGTGCTGTGTCCATGGCATAT
GAAGAGAAAGAGATCCATGATCAAAGCTGTCCTCTGGCAAATATCATCTGTGAATACTGTGGTACAATCCTCATCAGAGAACAGATGCCTAATCATTATG
ATCTGGACTGCCCAACAGCTCCAATCCCTTGCACATTCAGTGTTTTTGGCTGTCATGAAAAGATGCAGAGGAATCACTTGGCACGACACTTGCAAGAGAA
TACCCAGTTGCACATGAGACTGTTGGCCCAGGCTGTTCATAATGTTAACCTTGCTTTGCGTCCGTGCGATGCCGCCTCTCCATCCCGGGGATGTCGTCCA
GAGGACCCAAATTATGAGGAAACTATCAAACAGTTGGAGAGTCGCCTAGTAAGACAGGACCATCAGATCCGGGAGCTGACTGCCAAAATGGAAACTCAGA
GTATGTACGTGGGCGAGCTCAAACGGACCATTCGGACCCTGGAGGACAAGGTTGCCGAAATGGAAGCACAGCAGTGTAACGGGATCTACATTTGGAAGAT
TGGCAACTTTGGGATGCACTTGAAATCCCAAGAAGAGGAAAGACCTGTTGTCATCCATAGCCCTGGATTCTACACAGGCAGACCTGGGTACAAGCTGTGC
ATGCGCCTGCATCTTCAGTTACCGACAGCTCAGCGCTGTGCAAACTATATATCCCTTTTTGTCCACACAATGCAAGGAGAATATGACAGCCACCTCCCCT
GGCCCTTCCAGGGTACAATACGCCTTACAATTCTCGACCAGTCTGAAGCACTTATAAGGCAAAACCACGAAGAGGTCATGGACGCCAAACCAGAACTGCT
TGCCTTTCAGCGACCCACAATCCCACGGAACCCCAAAGGTTTTGGCTATGTAACATTTATGCACCTGGAAGCCTTAAGACAGGGAACCTTCATTAAGGAT
GATACATTACTAGTGCGCTGTGAAGTCTCTACCCGCTTTGACATGGGTGGCCTTCGGAAGGAGGGTTTCCAGCCACGAAGTACTGATGCGGGGGTGTAGG
CGGCCGC

Amino Acid Sequence

View Sequence

MSLLNCENSCGSSQSSSDCCAAMAASCSAAVKDDSVSGSASTGNLSSSFMEEIQGYDVEFDPPLESKYECPICHMALREAVQTPCGHRFCKACIIKSIRD
AGHKCPVDNEILLENQLFPDNFAKREILSLTVKCPNKGCLQKMELRHLEDHQVHCEFALVNCPQCQRPFQKCQVNTHIIEDCPRRQVSCVNCAVSMAYEE
KEIHDQSCPLANIICEYCGTILIREQMPNHYDLDCPTAPIPCTFSVFGCHEKMQRNHLARHLQENTQLHMRLLAQAVHNVNLALRPCDAASPSRGCRPED
PNYEETIKQLESRLVRQDHQIRELTAKMETQSMYVGELKRTIRTLEDKVAEMEAQQCNGIYIWKIGNFGMHLKSQEEERPVVIHSPGFYTGRPGYKLCMR
LHLQLPTAQRCANYISLFVHTMQGEYDSHLPWPFQGTIRLTILDQSEALIRQNHEEVMDAKPELLAFQRPTIPRNPKGFGYVTFMHLEALRQGTFIKDDT
LLVRCEVSTRFDMGGLRKEGFQPRSTDAGV

Vector Type

Mammalian

Antibiotic

Amp

Comments

Please mutate residue 74 from L to H in TRAF6 (mouse) from DU46985 and put into the vector from DU23477. Thanks.

100042 R 1000 1001 1047 19007

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