TRAF6 (mouse) | Ubiquitylation Resource

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Plasmid

pBABE puro Traf6 C70A (mouse)

Parent Plasmid

pBabe puro

Expressed

TRAF6 [C70A] (mouse)

DU Number

DU46995

Species

Mouse

Sequence of Insert

View Sequence

GGATCCATGAGTCTCTTAAACTGTGAGAACAGCTGCGGGTCCAGCCAGTCGTCCAGTGACTGCTGCGCTGCCATGGCCGCCTCCTGCAGCGCTGCAGTGA
AAGATGACAGCGTGAGTGGCTCTGCCAGCACCGGGAACCTCTCCAGCTCCTTCATGGAGGAGATCCAGGGCTACGATGTGGAGTTTGACCCACCTCTGGA
GAGCAAGTATGAGGCTCCCATCTGCTTGATGGCTTTACGGGAAGCAGTGCAAACACCATGTGGCCACAGGTTCTGCAAAGCCTGCATCATCAAATCCATA
AGGGATGCAGGGCACAAGTGCCCAGTTGACAATGAAATACTGCTGGAAAATCAACTGTTTCCCGACAATTTTGCAAAGCGAGAGATTCTTTCCCTGACGG
TAAAGTGCCCAAATAAAGGCTGTTTGCAAAAGATGGAACTGAGACATCTCGAGGATCATCAAGTACATTGTGAATTTGCTCTAGTGAATTGTCCCCAGTG
CCAACGTCCTTTCCAGAAGTGCCAGGTTAATACACACATTATTGAGGATTGTCCCAGGAGGCAGGTTTCTTGTGTAAACTGTGCTGTGTCCATGGCATAT
GAAGAGAAAGAGATCCATGATCAAAGCTGTCCTCTGGCAAATATCATCTGTGAATACTGTGGTACAATCCTCATCAGAGAACAGATGCCTAATCATTATG
ATCTGGACTGCCCAACAGCTCCAATCCCTTGCACATTCAGTGTTTTTGGCTGTCATGAAAAGATGCAGAGGAATCACTTGGCACGACACTTGCAAGAGAA
TACCCAGTTGCACATGAGACTGTTGGCCCAGGCTGTTCATAATGTTAACCTTGCTTTGCGTCCGTGCGATGCCGCCTCTCCATCCCGGGGATGTCGTCCA
GAGGACCCAAATTATGAGGAAACTATCAAACAGTTGGAGAGTCGCCTAGTAAGACAGGACCATCAGATCCGGGAGCTGACTGCCAAAATGGAAACTCAGA
GTATGTACGTGGGCGAGCTCAAACGGACCATTCGGACCCTGGAGGACAAGGTTGCCGAAATGGAAGCACAGCAGTGTAACGGGATCTACATTTGGAAGAT
TGGCAACTTTGGGATGCACTTGAAATCCCAAGAAGAGGAAAGACCTGTTGTCATCCATAGCCCTGGATTCTACACAGGCAGACCTGGGTACAAGCTGTGC
ATGCGCCTGCATCTTCAGTTACCGACAGCTCAGCGCTGTGCAAACTATATATCCCTTTTTGTCCACACAATGCAAGGAGAATATGACAGCCACCTCCCCT
GGCCCTTCCAGGGTACAATACGCCTTACAATTCTCGACCAGTCTGAAGCACTTATAAGGCAAAACCACGAAGAGGTCATGGACGCCAAACCAGAACTGCT
TGCCTTTCAGCGACCCACAATCCCACGGAACCCCAAAGGTTTTGGCTATGTAACATTTATGCACCTGGAAGCCTTAAGACAGGGAACCTTCATTAAGGAT
GATACATTACTAGTGCGCTGTGAAGTCTCTACCCGCTTTGACATGGGTGGCCTTCGGAAGGAGGGTTTCCAGCCACGAAGTACTGATGCGGGGGTGTAGG
CGGCCGC

Amino Acid Sequence

View Sequence

MSLLNCENSCGSSQSSSDCCAAMAASCSAAVKDDSVSGSASTGNLSSSFMEEIQGYDVEFDPPLESKYEAPICLMALREAVQTPCGHRFCKACIIKSIRD
AGHKCPVDNEILLENQLFPDNFAKREILSLTVKCPNKGCLQKMELRHLEDHQVHCEFALVNCPQCQRPFQKCQVNTHIIEDCPRRQVSCVNCAVSMAYEE
KEIHDQSCPLANIICEYCGTILIREQMPNHYDLDCPTAPIPCTFSVFGCHEKMQRNHLARHLQENTQLHMRLLAQAVHNVNLALRPCDAASPSRGCRPED
PNYEETIKQLESRLVRQDHQIRELTAKMETQSMYVGELKRTIRTLEDKVAEMEAQQCNGIYIWKIGNFGMHLKSQEEERPVVIHSPGFYTGRPGYKLCMR
LHLQLPTAQRCANYISLFVHTMQGEYDSHLPWPFQGTIRLTILDQSEALIRQNHEEVMDAKPELLAFQRPTIPRNPKGFGYVTFMHLEALRQGTFIKDDT
LLVRCEVSTRFDMGGLRKEGFQPRSTDAGV*

Vector Type

Mammalian

Antibiotic

Amp

Comments

Please introduce mutation [C70A] into TRAF6 (mouse) from DU43538 and put into the vector DU47224. Please remove the FLAG-tag. Thanks.

100042 R 1000 1001 1047 19007

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