This reagent and more can be found at our sister site MRC PPU Reagents & Services 
Request Now
Plasmid
pSc-B-USP42 (no stop) C120A
Parent Plasmid
pSC-B
DU Number
DU24718
Genbank
NM_032172.2
Species
Human
Sequence of Insert
View Sequence
GGATCCATGACCATAGTTGACAAAGCTTCTGAATCTTCAGACCCATCAGC
CTATCAGAATCAGCCTGGCAGCTCCGAGGCAGTCTCACCTGGAGACATGG
ATGCAGGTTCTGCCAGCTGGGGTGCTGTGTCTTCATTGAATGATGTGTCA
AATCACACACTTTCTTTAGGACCAGTACCTGGTGCTGTAGTTTATTCGAG
TTCATCTGTACCTGATAAATCAAAACCATCACCACAAAAGGATCAAGCCC
TAGGTGATGGCATCGCTCCTCCACAGAAAGTTCTTTTCCCATCTGAGAAG
ATTTGTCTTAAGTGGCAACAAACTCATAGAGTTGGAGCTGGGCTCCAGAA
TTTGGGCAATACCgcTTTTGCCAATGCAGCACTGCAGTGTTTAACCTACA
CACCACCTCTTGCCAATTACATGCTATCACATGAACACTCCAAAACATGT
CATGCAGAAGGCTTTTGTATGATGTGTACAATGCAAGCACATATTACCCA
GGCACTCAGTAATCCTGGGGACGTTATTAAACCAATGTTTGTCATCAATG
AGATGCGGCGTATAGCTAGGCACTTCCGTTTTGGAAACCAAGAAGATGCC
CATGAATTCCTTCAATACACTGTTGATGCTATGCAGAAAGCATGCTTGAA
TGGCAGCAATAAATTAGACAGACACACCCAGGCCACCACTCTTGTTTGTC
AGATATTTGGAGGATACCTAAGATCTAGAGTCAAATGTTTAAATTGCAAG
GGCGTTTCAGATACTTTTGATCCATATCTTGATATAACATTGGAGATAAA
GGCTGCTCAGAGTGTCAACAAGGCATTGGAGCAGTTTGTGAAGCCGGAAC
AGCTTGATGGAGAAAACTCGTACAAGTGCAGCAAGTGTAAAAAGATGGTT
CCAGCTTCAAAGAGGTTCACTATCCATAGATCCTCTAATGTTCTTACACT
TTCTCTGAAACGTTTTGCAAATTTTACCGGTGGAAAAATTGCTAAGGATG
TGAAATACCCTGAGTATCTTGATATTCGGCCATATATGTCTCAACCCAAC
GGAGAGCCAATTGTCTACGTCTTGTATGCAGTGCTGGTCCACACTGGTTT
TAATTGCCATGCTGGCCATTACTTCTGCTACATAAAAGCTAGCAATGGCC
TCTGGTATCAAATGAATGACTCCATTGTATCTACCAGTGATATTAGATCG
GTACTCAGCCAACAAGCCTATGTGCTCTTTTATATCAGGTCCCATGATGT
GAAAAATGGAGGTGAACTTACTCATCCCACCCATAGCCCCGGCCAGTCCT
CTCCCCGCCCCGTCATCAGTCAGCGGGTTGTCACCAACAAACAGGCTGCG
CCAGGCTTTATCGGACCACAGCTTCCCTCTCACATGATAAAGAATCCACC
TCACTTAAATGGGACTGGACCATTGAAAGACACGCCAAGCAGTTCCATGT
CGAGTCCTAACGGGAATTCCAGTGTCAACAGGGCTAGTCCTGTTAATGCT
TCAGCTTCTGTCCAAAACTGGTCAGTTAATAGGTCCTCAGTGATCCCAGA
ACATCCTAAGAAACAAAAAATTACAATCAGTATTCACAACAAGTTGCCTG
TTCGCCAGTGTCAGTCTCAACCTAACCTTCATAGTAATTCTTTGGAGAAC
CCTACCAAGCCCGTTCCCTCTTCTACCATTACCAATTCTGCAGTACAGTC
TACCTCGAACGCATCTACGATGTCAGTTTCTAGTAAAGTAACAAAACCGA
TCCCCCGCAGTGAATCCTGCTCCCAGCCCGTGATGAATGGCAAATCCAAG
CTGAACTCCAGCGTGCTGGTGCCCTATGGCGCCGAGTCCTCTGAGGACTC
TGACGAGGAGTCAAAGGGGCTGGGCAAGGAGAATGGGATTGGTACGATTG
TGAGCTCCCACTCTCCCGGCCAAGATGCCGAAGATGAGGAGGCCACTCCG
CACGAGCTTCAAGAACCCATGACCCTAAACGGTGCTAATAGTGCAGACAG
CGACAGTGACCCGAAAGAAAACGGCCTAGCGCCTGATGGTGCCAGCTGCC
AAGGCCAGCCTGCCCTGCACTCAGAAAATCCCTTTGCTAAGGCAAACGGT
CTTCCTGGAAAGTTGATGCCTGCTCCTTTGCTGTCTCTCCCAGAAGACAA
AATCTTAGAGACCTTCAGGCTTAGCAACAAACTGAAAGGCTCGACGGATG
AAATGAGTGCACCTGGAGCAGAGAGGGGCCCTCCCGAGGACCGCGACGCC
GAGCCTCAGCCTGGCAGCCCCGCCGCCGAATCCCTGGAGGAGCCAGATGC
GGCCGCCGGCCTCAGCAGCACCAAGAAGGCTCCGCCGCCCCGCGATCCCG
GCACCCCCGCTACCAAAGAAGGCGCCTGGGAGGCCATGGCCGTCGCCCCC
GAGGAGCCTCCGCCCAGCGCCGGCGAGGACATCGTGGGGGACACAGCACC
CCCTGACCTGTGTGATCCCGGGAGCTTAACAGGCGATGCGAGCCCGTTGT
CCCAGGACGCAAAGGGGATGATCGCGGAGGGCCCGCGGGACTCGGCGTTG
GCGGAAGCCCCGGAAGGGTTGAGTCCGGCTCCGCCTGCGCGGTCGGAGGA
GCCCTGCGAGCAGCCACTCCTTGTTCACCCCAGCGGGGACCACGCCCGGG
ACGCTCAGGACCCATCCCAGAGCTTGGGCGCACCCGAGGCCGCAGAGCGG
CCGCCAGCTCCTGTGCTGGACATGGCCCCGGCCGGTCACCCGGAAGGGGA
CGCTGAGCCTAGCCCCGGCGAGAGGGTCGAGGACGCCGCGGCGCCGAAAG
CCCCAGGCCCTTCCCCAGCGAAGGAGAAAATCGGCAGCCTCAGAAAGGTG
GACCGAGGCCACTACCGCAGCCGGAGAGAGCGCTCGTCCAGCGGGGAGCC
CGCCAGAGAGAGCAGGAGCAAGACTGAGGGCCACCGTCACCGGCGGCGCC
GCACCTGCCCCCGGGAGCGCGACCGCCAGGACCGCCACGCCCCGGAGCAC
CACCCCGGCCACGGCGACAGGCTCAGCCCTGGCGAGCGCCGCTCTCTGGG
CAGGTGCAGTCACCACCACTCCCGACACCGGAGCGGGGTGGAGCTGGACT
GGGTCAGACACCACTACACCGAGGGCGAGCGTGGCTGGGGCCGGGAGAAG
TTCTACCCCGACAGGCCGCGCTGGGACAGGTGCCGGTACTACCATGACAG
GTACGCCCTGTACGCTGCCCGGGACTGGAAGCCCTTCCACGGCGGCCGCG
AGCACGAGCGGGCCGGGCTGCACGAGCGGCCGCACAAGGACCACAACCGG
GGCCGTAGGGGCTGCGAGCCGGCCCGGGAGAGGGAGCGGCACCGCCCCAG
CAGCCCCCGCGCAGGCGCGCCCCACGCCCTCGCCCCGCACCCCGACCGCT
TCTCCCACGACAGAACTGCACTTGTAGCCGGAGACAACTGTAACCTCTCT
GATCGGTTTCACGAACACGAAAATGGAAAGTCCCGGAAACGGAGACACGA
CAGTGTGGAGAACAGTGACAGTCATGTTGAAAAGAAAGCCCGGAGGAGCG
AACAGAAGGACCCTCTAGAAGAGCCTAAAGCAAAGAAGCACAAAAAATCA
AAGAAGAAAAAGAAATCCAAAGACAAACACCGAGACCGCGACTCCAGGCA
TCAGCAGGACTCAGACCTCTCAGCAGCGTGCTCTGACGCTGACCTCCACA
GACACAAAAAAAAGAAGAAGAAAAAGAAGAGACATTCAAGAAAATCAGAG
GACTTTGTTAAAGATTCAGAACTGCACTTACCCAGGGTCACCAGCTTGGA
GACTGTCGCCCAGTTCCGGAGAGCCCAGGGTGGCTTTCCTCTCTCTGGTG
GCCCGCCTCTGGAAGGCGTCGGACCTTTCCGTGAGAAAACGAAACACTTA
CGGATGGAAAGCAGGGATGACAGGTGTCGTCTCTTTGAGTATGGCCAGGG
TGATCTCGAG
CTATCAGAATCAGCCTGGCAGCTCCGAGGCAGTCTCACCTGGAGACATGG
ATGCAGGTTCTGCCAGCTGGGGTGCTGTGTCTTCATTGAATGATGTGTCA
AATCACACACTTTCTTTAGGACCAGTACCTGGTGCTGTAGTTTATTCGAG
TTCATCTGTACCTGATAAATCAAAACCATCACCACAAAAGGATCAAGCCC
TAGGTGATGGCATCGCTCCTCCACAGAAAGTTCTTTTCCCATCTGAGAAG
ATTTGTCTTAAGTGGCAACAAACTCATAGAGTTGGAGCTGGGCTCCAGAA
TTTGGGCAATACCgcTTTTGCCAATGCAGCACTGCAGTGTTTAACCTACA
CACCACCTCTTGCCAATTACATGCTATCACATGAACACTCCAAAACATGT
CATGCAGAAGGCTTTTGTATGATGTGTACAATGCAAGCACATATTACCCA
GGCACTCAGTAATCCTGGGGACGTTATTAAACCAATGTTTGTCATCAATG
AGATGCGGCGTATAGCTAGGCACTTCCGTTTTGGAAACCAAGAAGATGCC
CATGAATTCCTTCAATACACTGTTGATGCTATGCAGAAAGCATGCTTGAA
TGGCAGCAATAAATTAGACAGACACACCCAGGCCACCACTCTTGTTTGTC
AGATATTTGGAGGATACCTAAGATCTAGAGTCAAATGTTTAAATTGCAAG
GGCGTTTCAGATACTTTTGATCCATATCTTGATATAACATTGGAGATAAA
GGCTGCTCAGAGTGTCAACAAGGCATTGGAGCAGTTTGTGAAGCCGGAAC
AGCTTGATGGAGAAAACTCGTACAAGTGCAGCAAGTGTAAAAAGATGGTT
CCAGCTTCAAAGAGGTTCACTATCCATAGATCCTCTAATGTTCTTACACT
TTCTCTGAAACGTTTTGCAAATTTTACCGGTGGAAAAATTGCTAAGGATG
TGAAATACCCTGAGTATCTTGATATTCGGCCATATATGTCTCAACCCAAC
GGAGAGCCAATTGTCTACGTCTTGTATGCAGTGCTGGTCCACACTGGTTT
TAATTGCCATGCTGGCCATTACTTCTGCTACATAAAAGCTAGCAATGGCC
TCTGGTATCAAATGAATGACTCCATTGTATCTACCAGTGATATTAGATCG
GTACTCAGCCAACAAGCCTATGTGCTCTTTTATATCAGGTCCCATGATGT
GAAAAATGGAGGTGAACTTACTCATCCCACCCATAGCCCCGGCCAGTCCT
CTCCCCGCCCCGTCATCAGTCAGCGGGTTGTCACCAACAAACAGGCTGCG
CCAGGCTTTATCGGACCACAGCTTCCCTCTCACATGATAAAGAATCCACC
TCACTTAAATGGGACTGGACCATTGAAAGACACGCCAAGCAGTTCCATGT
CGAGTCCTAACGGGAATTCCAGTGTCAACAGGGCTAGTCCTGTTAATGCT
TCAGCTTCTGTCCAAAACTGGTCAGTTAATAGGTCCTCAGTGATCCCAGA
ACATCCTAAGAAACAAAAAATTACAATCAGTATTCACAACAAGTTGCCTG
TTCGCCAGTGTCAGTCTCAACCTAACCTTCATAGTAATTCTTTGGAGAAC
CCTACCAAGCCCGTTCCCTCTTCTACCATTACCAATTCTGCAGTACAGTC
TACCTCGAACGCATCTACGATGTCAGTTTCTAGTAAAGTAACAAAACCGA
TCCCCCGCAGTGAATCCTGCTCCCAGCCCGTGATGAATGGCAAATCCAAG
CTGAACTCCAGCGTGCTGGTGCCCTATGGCGCCGAGTCCTCTGAGGACTC
TGACGAGGAGTCAAAGGGGCTGGGCAAGGAGAATGGGATTGGTACGATTG
TGAGCTCCCACTCTCCCGGCCAAGATGCCGAAGATGAGGAGGCCACTCCG
CACGAGCTTCAAGAACCCATGACCCTAAACGGTGCTAATAGTGCAGACAG
CGACAGTGACCCGAAAGAAAACGGCCTAGCGCCTGATGGTGCCAGCTGCC
AAGGCCAGCCTGCCCTGCACTCAGAAAATCCCTTTGCTAAGGCAAACGGT
CTTCCTGGAAAGTTGATGCCTGCTCCTTTGCTGTCTCTCCCAGAAGACAA
AATCTTAGAGACCTTCAGGCTTAGCAACAAACTGAAAGGCTCGACGGATG
AAATGAGTGCACCTGGAGCAGAGAGGGGCCCTCCCGAGGACCGCGACGCC
GAGCCTCAGCCTGGCAGCCCCGCCGCCGAATCCCTGGAGGAGCCAGATGC
GGCCGCCGGCCTCAGCAGCACCAAGAAGGCTCCGCCGCCCCGCGATCCCG
GCACCCCCGCTACCAAAGAAGGCGCCTGGGAGGCCATGGCCGTCGCCCCC
GAGGAGCCTCCGCCCAGCGCCGGCGAGGACATCGTGGGGGACACAGCACC
CCCTGACCTGTGTGATCCCGGGAGCTTAACAGGCGATGCGAGCCCGTTGT
CCCAGGACGCAAAGGGGATGATCGCGGAGGGCCCGCGGGACTCGGCGTTG
GCGGAAGCCCCGGAAGGGTTGAGTCCGGCTCCGCCTGCGCGGTCGGAGGA
GCCCTGCGAGCAGCCACTCCTTGTTCACCCCAGCGGGGACCACGCCCGGG
ACGCTCAGGACCCATCCCAGAGCTTGGGCGCACCCGAGGCCGCAGAGCGG
CCGCCAGCTCCTGTGCTGGACATGGCCCCGGCCGGTCACCCGGAAGGGGA
CGCTGAGCCTAGCCCCGGCGAGAGGGTCGAGGACGCCGCGGCGCCGAAAG
CCCCAGGCCCTTCCCCAGCGAAGGAGAAAATCGGCAGCCTCAGAAAGGTG
GACCGAGGCCACTACCGCAGCCGGAGAGAGCGCTCGTCCAGCGGGGAGCC
CGCCAGAGAGAGCAGGAGCAAGACTGAGGGCCACCGTCACCGGCGGCGCC
GCACCTGCCCCCGGGAGCGCGACCGCCAGGACCGCCACGCCCCGGAGCAC
CACCCCGGCCACGGCGACAGGCTCAGCCCTGGCGAGCGCCGCTCTCTGGG
CAGGTGCAGTCACCACCACTCCCGACACCGGAGCGGGGTGGAGCTGGACT
GGGTCAGACACCACTACACCGAGGGCGAGCGTGGCTGGGGCCGGGAGAAG
TTCTACCCCGACAGGCCGCGCTGGGACAGGTGCCGGTACTACCATGACAG
GTACGCCCTGTACGCTGCCCGGGACTGGAAGCCCTTCCACGGCGGCCGCG
AGCACGAGCGGGCCGGGCTGCACGAGCGGCCGCACAAGGACCACAACCGG
GGCCGTAGGGGCTGCGAGCCGGCCCGGGAGAGGGAGCGGCACCGCCCCAG
CAGCCCCCGCGCAGGCGCGCCCCACGCCCTCGCCCCGCACCCCGACCGCT
TCTCCCACGACAGAACTGCACTTGTAGCCGGAGACAACTGTAACCTCTCT
GATCGGTTTCACGAACACGAAAATGGAAAGTCCCGGAAACGGAGACACGA
CAGTGTGGAGAACAGTGACAGTCATGTTGAAAAGAAAGCCCGGAGGAGCG
AACAGAAGGACCCTCTAGAAGAGCCTAAAGCAAAGAAGCACAAAAAATCA
AAGAAGAAAAAGAAATCCAAAGACAAACACCGAGACCGCGACTCCAGGCA
TCAGCAGGACTCAGACCTCTCAGCAGCGTGCTCTGACGCTGACCTCCACA
GACACAAAAAAAAGAAGAAGAAAAAGAAGAGACATTCAAGAAAATCAGAG
GACTTTGTTAAAGATTCAGAACTGCACTTACCCAGGGTCACCAGCTTGGA
GACTGTCGCCCAGTTCCGGAGAGCCCAGGGTGGCTTTCCTCTCTCTGGTG
GCCCGCCTCTGGAAGGCGTCGGACCTTTCCGTGAGAAAACGAAACACTTA
CGGATGGAAAGCAGGGATGACAGGTGTCGTCTCTTTGAGTATGGCCAGGG
TGATCTCGAG
Amino Acid Sequence
View Sequence
MTIVDKASESSDPSAYQNQPGSSEAVSPGDMDAGSASWGAVSSLNDVSNH
TLSLGPVPGAVVYSSSSVPDKSKPSPQKDQALGDGIAPPQKVLFPSEKIC
LKWQQTHRVGAGLQNLGNTAFANAALQCLTYTPPLANYMLSHEHSKTCHA
EGFCMMCTMQAHITQALSNPGDVIKPMFVINEMRRIARHFRFGNQEDAHE
FLQYTVDAMQKACLNGSNKLDRHTQATTLVCQIFGGYLRSRVKCLNCKGV
SDTFDPYLDITLEIKAAQSVNKALEQFVKPEQLDGENSYKCSKCKKMVPA
SKRFTIHRSSNVLTLSLKRFANFTGGKIAKDVKYPEYLDIRPYMSQPNGE
PIVYVLYAVLVHTGFNCHAGHYFCYIKASNGLWYQMNDSIVSTSDIRSVL
SQQAYVLFYIRSHDVKNGGELTHPTHSPGQSSPRPVISQRVVTNKQAAPG
FIGPQLPSHMIKNPPHLNGTGPLKDTPSSSMSSPNGNSSVNRASPVNASA
SVQNWSVNRSSVIPEHPKKQKITISIHNKLPVRQCQSQPNLHSNSLENPT
KPVPSSTITNSAVQSTSNASTMSVSSKVTKPIPRSESCSQPVMNGKSKLN
SSVLVPYGAESSEDSDEESKGLGKENGIGTIVSSHSPGQDAEDEEATPHE
LQEPMTLNGANSADSDSDPKENGLAPDGASCQGQPALHSENPFAKANGLP
GKLMPAPLLSLPEDKILETFRLSNKLKGSTDEMSAPGAERGPPEDRDAEP
QPGSPAAESLEEPDAAAGLSSTKKAPPPRDPGTPATKEGAWEAMAVAPEE
PPPSAGEDIVGDTAPPDLCDPGSLTGDASPLSQDAKGMIAEGPRDSALAE
APEGLSPAPPARSEEPCEQPLLVHPSGDHARDAQDPSQSLGAPEAAERPP
APVLDMAPAGHPEGDAEPSPGERVEDAAAPKAPGPSPAKEKIGSLRKVDR
GHYRSRRERSSSGEPARESRSKTEGHRHRRRRTCPRERDRQDRHAPEHHP
GHGDRLSPGERRSLGRCSHHHSRHRSGVELDWVRHHYTEGERGWGREKFY
PDRPRWDRCRYYHDRYALYAARDWKPFHGGREHERAGLHERPHKDHNRGR
RGCEPARERERHRPSSPRAGAPHALAPHPDRFSHDRTALVAGDNCNLSDR
FHEHENGKSRKRRHDSVENSDSHVEKKARRSEQKDPLEEPKAKKHKKSKK
KKKSKDKHRDRDSRHQQDSDLSAACSDADLHRHKKKKKKKKRHSRKSEDF
VKDSELHLPRVTSLETVAQFRRAQGGFPLSGGPPLEGVGPFREKTKHLRM
ESRDDRCRLFEYGQGD
TLSLGPVPGAVVYSSSSVPDKSKPSPQKDQALGDGIAPPQKVLFPSEKIC
LKWQQTHRVGAGLQNLGNTAFANAALQCLTYTPPLANYMLSHEHSKTCHA
EGFCMMCTMQAHITQALSNPGDVIKPMFVINEMRRIARHFRFGNQEDAHE
FLQYTVDAMQKACLNGSNKLDRHTQATTLVCQIFGGYLRSRVKCLNCKGV
SDTFDPYLDITLEIKAAQSVNKALEQFVKPEQLDGENSYKCSKCKKMVPA
SKRFTIHRSSNVLTLSLKRFANFTGGKIAKDVKYPEYLDIRPYMSQPNGE
PIVYVLYAVLVHTGFNCHAGHYFCYIKASNGLWYQMNDSIVSTSDIRSVL
SQQAYVLFYIRSHDVKNGGELTHPTHSPGQSSPRPVISQRVVTNKQAAPG
FIGPQLPSHMIKNPPHLNGTGPLKDTPSSSMSSPNGNSSVNRASPVNASA
SVQNWSVNRSSVIPEHPKKQKITISIHNKLPVRQCQSQPNLHSNSLENPT
KPVPSSTITNSAVQSTSNASTMSVSSKVTKPIPRSESCSQPVMNGKSKLN
SSVLVPYGAESSEDSDEESKGLGKENGIGTIVSSHSPGQDAEDEEATPHE
LQEPMTLNGANSADSDSDPKENGLAPDGASCQGQPALHSENPFAKANGLP
GKLMPAPLLSLPEDKILETFRLSNKLKGSTDEMSAPGAERGPPEDRDAEP
QPGSPAAESLEEPDAAAGLSSTKKAPPPRDPGTPATKEGAWEAMAVAPEE
PPPSAGEDIVGDTAPPDLCDPGSLTGDASPLSQDAKGMIAEGPRDSALAE
APEGLSPAPPARSEEPCEQPLLVHPSGDHARDAQDPSQSLGAPEAAERPP
APVLDMAPAGHPEGDAEPSPGERVEDAAAPKAPGPSPAKEKIGSLRKVDR
GHYRSRRERSSSGEPARESRSKTEGHRHRRRRTCPRERDRQDRHAPEHHP
GHGDRLSPGERRSLGRCSHHHSRHRSGVELDWVRHHYTEGERGWGREKFY
PDRPRWDRCRYYHDRYALYAARDWKPFHGGREHERAGLHERPHKDHNRGR
RGCEPARERERHRPSSPRAGAPHALAPHPDRFSHDRTALVAGDNCNLSDR
FHEHENGKSRKRRHDSVENSDSHVEKKARRSEQKDPLEEPKAKKHKKSKK
KKKSKDKHRDRDSRHQQDSDLSAACSDADLHRHKKKKKKKKRHSRKSEDF
VKDSELHLPRVTSLETVAQFRRAQGGFPLSGGPPLEGVGPFREKTKHLRM
ESRDDRCRLFEYGQGD
Vector Type
Other
Antibiotic
Amp; Kan
100042 R 1000 1001 1047 19007
Download datasheet