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Plasmid
pcDNA5 FRT/TO BirA R118G-USP43 C110A-HA
Parent Plasmid
pcDNA5 FRT/TO BirA R118G MCS HA
Expressed
BirA R118G-USP43 C110A-HA
DU Number
DU50016
Genbank
NM_153210.4
Tag
HA
BirA
Species
Human
Sequence of Insert
View Sequence
GAATTCATGGACCTGGGCCCCGGGGACGCGGCAGGAGGGGGACCGCTCGCGCCCCGGCCCCGCCGCCGCCGCTCCCTGCGCCGCCTGTTCAGCCGCTTCCTGCTGGCGCTGGGCAGCCGCTCACGCCCCGGGGACTCACCGCCCCGGCCCCAGCCGGGACACTGTGATGGCGACGGTGAGGGGGGCTTCGCCTGCGCCCCGGGCCCAGTTCCAGCGGCCCCCGGGAGCCCCGGGGAGGAACGCCCGCCCGGACCCCAGCCCCAGCTCCAGCTCCCCGCCGGCGATGGGGCTCGGCCTCCGGGCGCTCAGGGCTTGAAGAACCACGGCAACACCGCCTTCATGAACGCGGTGGTGCAGTGTCTCAGCAACACCGACCTGCTGGCCGAGTTCCTGGCGCTGGGGCGCTACCGGGCGGCTCCGGGCCGCGCCGAGGTCACCGAGCAGCTGGCGGCGCTGGTGCGCGCGCTCTGGACTCGCGAATACACGCCCCAACTTTCCGCGGAGTTCAAGAATGCAGTTTCCAAGTACGGCTCTCAGTTCCAAGGCAATTCCCAGCACGACGCCCTGGAGTTCCTGCTCTGGTTGCTGGATCGTGTACATGAGGACCTGGAGGGTTCATCCCGAGGGCCGGTGTCGGAGAAGCTTCCGCCTGAAGCCACTAAAACCTCTGAGAACTGCCTGTCACCATCAGCTCAGCTTCCTCTAGGTCAAAGCTTTGTGCAAAGCCACTTTCAAGCACAATATAGATCTTCCTTGACTTGTCCCCACTGCCTGAAACAGAGCAACACCTTTGATCCTTTCCTGTGTGTGTCCCTACCTATCCCCTTGCGCCAGACGAGGTTCTTGAGTGTCACCTTGGTCTTCCCCTCTAAGAGCCAGCGGTTCCTGCGGGTTGGCCTGGCCGTGCCGATCCTCAGCACAGTGGCAGCCCTGAGGAAGATGGTTGCAGAGGAAGGAGGCGTCCCTGCAGATGAGGTGATCTTGGTTGAACTGTATCCCAGTGGATTCCAGCGGTCTTTCTTTGATGAAGAGGACCTGAATACCATCGCAGAGGGAGATAATGTGTATGCCTTTCAAGTTCCTCCCTCACCCAGCCAGGGGACTCTCTCAGCTCATCCACTGGGTCTGTCGGCCTCCCCACGCCTGGCAGCCCGTGAGGGCCAGCGATTCTCCCTCTCTCTCCACAGTGAGAGCAAGGTGCTAATCCTCTTCTGTAACTTGGTGGGGTCAGGGCAGCAGGCTAGCAGGTTTGGGCCACCCTTCCTGATAAGGGAAGACAGAGCTGTTTCCTGGGCCCAGCTCCAGCAGTCTATCCTCAGCAAGGTCCGCCATCTTATGAAGAGTGAGGCCCCTGTACAGAACCTGGGGTCTCTGTTCTCCATCCGTGTTGTGGGACTCTCTGTGGCCTGCAGCTATTTGTCTCCGAAGGACAGTCGGCCCCTCTGTCACTGGGCAGTTGACAGGGTTTTGCATCTCAGGAGGCCAGGAGGCCCTCCACATGTCAAGCTGGCGGTGGAGTGGGATAGCTCTGTCAAGGAGCGCCTGTTCGGGAGCCTCCAGGAGGAGCGAGCGCAGGATGCCGACAGTGTGTGGCAGCAGCAGCAGGCGCATCAGCAGCACAGCTGTACCTTGGATGAATGTTTTCAGTTCTACACCAAGGAGGAGCAGCTGGCCCAGGATGACGCCTGGAAGTGTCCTCACTGCCAAGTCCTGCAGCAGGGGATGGTGAAGCTGAGTTTGTGGACGCTGCCTGACATCCTCATCATCCACCTCAAAAGGTTCTGCCAGGTGGGCGAGAGAAGAAACAAGCTCTCCACGCTGGTGAAGTTTCCGCTCTCTGGACTCAACATGGCTCCCCATGTGGCCCAGAGAAGCACCAGCCCTGAGGCAGGACTGGGCCCCTGGCCTTCCTGGAAGCAGCCGGACTGCCTGCCCACCAGTTACCCGCTGGACTTCCTGTACGACCTGTATGCCGTCTGCAACCACCATGGCAACCTGCAAGGTGGGCATTACACAGCCTACTGCCGGAACTCTCTGGATGGCCAGTGGTACAGTTATGATGACAGCACGGTGGAACCGCTTCGAGAAGATGAGGTCAACACCAGAGGGGCTTATATCCTGTTCTATCAGAAGCGGAACAGCATCCCTCCCTGGTCAGCCAGCAGCTCCATGAGAGGCTCTACCAGCTCCTCCCTGTCTGATCACTGGCTCTTACGGCTCGGGAGCCACGCTGGCAGCACAAGGGGAAGCCTGCTGTCCTGGAGCTCTGCCCCCTGCCCCTCCCTGCCCCAGGTTCCTGACTCTCCCATCTTCACCAACAGCCTCTGCAATCAGGAAAAGGGAGGGTTGGAGCCCAGGCGTTTGGTACGGGGCGTGAAAGGCAGAAGCATTAGCATGAAGGCACCCACCACTTCCCGAGCCAAGCAGGGACCATTCAAGACCATGCCTCTGCGGTGGTCCTTTGGATCCAAGGAGAAACCACCAGGTGCCTCCGTCGAGTTGGTGGAGTACTTGGAATCCAGACGAAGACCTCGGTCCACGAGCCAGTCCATTGTGTCGCTGTTGACGGGCACTGCGGGTGAGGATGAGAAGTCAGCATCGCCGAGGTCCAACGTCGCCCTTCCTGCTAACAGCGAAGATGGTGGGCGGGCCATTGAAAGAGGTCCAGCCGGGGTGCCCTGTCCCTCGGCTCAACCCAACCACTGTCTGGCCCCTGGAAACTCAGATGGTCCAAACACAGCAAGGAAACTCAAGGAAAATGCAGGGCAGGACATCAAGCTTCCCAGAAAGTTTGACCTGCCTCTCACTGTGATGCCTTCAGTGGAGCATGAGAAACCAGCTCGACCGGAGGGCCAGAAGGCCATGAACTGGAAGGAGAGCTTCCAGATGGGAAGCAAAAGCAGCCCACCCTCCCCCTATATGGGATTCTCTGGAAACAGCAAAGACAGTCGCCGAGGCACCTCTGAGCTAGACAGACCCCTGCAGGGGACACTCACCCTTCTGAGGTCCGTGTTTCGGAAGAAGGAGAACAGGAGGAATGAGAGGGCAGAGGTCTCTCCACAGGTGCCCCCCGTCTCCCTGGTGAGTGGCGGGCTGAGCCCTGCCATGGACGGGCAGGCTCCAGGCTCACCTCCTGCCCTCAGGATCCCAGAGGGCCTGGCCAGGGGCCTGGGCAGCCGGCTCGAGAGGGATGTCTGGTCAGCCCCCAGCTCTCTCCGCCTCCCTCGTAAAGCCAGCAGGGCCCCGAGAGGCAGTGCACTGGGCATGTCACAAAGGACTGTTCCAGGGGAGCAGGCTTCTTATGGCACCTTTCAGAGAGTCAAATATCACACTCTTTCTTTAGGTCGAAAGAAAACCTTACCGGAGTCCAGCTTTGCGGCCGC
Amino Acid Sequence
View Sequence
MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRDWGVDV
FTVPGKGYSLPEPIQLLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGE
LKSGDACIAEYQQAGRGGRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLS
LVIGIVMAEVLRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQ
IVIGAGINMAMRRVEESVVNQGWITLQEAGINLDRNTLAAMLIRELRAAL
ELFEQEGLAPYLSRWEKLDNFINRPVKLIIGDKEIFGISRGIDKQGALLL
EQDGIIKPWMGGEISLRSAEKGSPGEFMDLGPGDAAGGGPLAPRPRRRRS
LRRLFSRFLLALGSRSRPGDSPPRPQPGHCDGDGEGGFACAPGPVPAAPG
SPGEERPPGPQPQLQLPAGDGARPPGAQGLKNHGNTAFMNAVVQCLSNTD
LLAEFLALGRYRAAPGRAEVTEQLAALVRALWTREYTPQLSAEFKNAVSK
YGSQFQGNSQHDALEFLLWLLDRVHEDLEGSSRGPVSEKLPPEATKTSEN
CLSPSAQLPLGQSFVQSHFQAQYRSSLTCPHCLKQSNTFDPFLCVSLPIP
LRQTRFLSVTLVFPSKSQRFLRVGLAVPILSTVAALRKMVAEEGGVPADE
VILVELYPSGFQRSFFDEEDLNTIAEGDNVYAFQVPPSPSQGTLSAHPLG
LSASPRLAAREGQRFSLSLHSESKVLILFCNLVGSGQQASRFGPPFLIRE
DRAVSWAQLQQSILSKVRHLMKSEAPVQNLGSLFSIRVVGLSVACSYLSP
KDSRPLCHWAVDRVLHLRRPGGPPHVKLAVEWDSSVKERLFGSLQEERAQ
DADSVWQQQQAHQQHSCTLDECFQFYTKEEQLAQDDAWKCPHCQVLQQGM
VKLSLWTLPDILIIHLKRFCQVGERRNKLSTLVKFPLSGLNMAPHVAQRS
TSPEAGLGPWPSWKQPDCLPTSYPLDFLYDLYAVCNHHGNLQGGHYTAYC
RNSLDGQWYSYDDSTVEPLREDEVNTRGAYILFYQKRNSIPPWSASSSMR
GSTSSSLSDHWLLRLGSHAGSTRGSLLSWSSAPCPSLPQVPDSPIFTNSL
CNQEKGGLEPRRLVRGVKGRSISMKAPTTSRAKQGPFKTMPLRWSFGSKE
KPPGASVELVEYLESRRRPRSTSQSIVSLLTGTAGEDEKSASPRSNVALP
ANSEDGGRAIERGPAGVPCPSAQPNHCLAPGNSDGPNTARKLKENAGQDI
KLPRKFDLPLTVMPSVEHEKPARPEGQKAMNWKESFQMGSKSSPPSPYMG
FSGNSKDSRRGTSELDRPLQGTLTLLRSVFRKKENRRNERAEVSPQVPPV
SLVSGGLSPAMDGQAPGSPPALRIPEGLARGLGSRLERDVWSAPSSLRLP
RKASRAPRGSALGMSQRTVPGEQASYGTFQRVKYHTLSLGRKKTLPESSF
AAAYPYDVPDYA*
FTVPGKGYSLPEPIQLLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGE
LKSGDACIAEYQQAGRGGRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLS
LVIGIVMAEVLRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQ
IVIGAGINMAMRRVEESVVNQGWITLQEAGINLDRNTLAAMLIRELRAAL
ELFEQEGLAPYLSRWEKLDNFINRPVKLIIGDKEIFGISRGIDKQGALLL
EQDGIIKPWMGGEISLRSAEKGSPGEFMDLGPGDAAGGGPLAPRPRRRRS
LRRLFSRFLLALGSRSRPGDSPPRPQPGHCDGDGEGGFACAPGPVPAAPG
SPGEERPPGPQPQLQLPAGDGARPPGAQGLKNHGNTAFMNAVVQCLSNTD
LLAEFLALGRYRAAPGRAEVTEQLAALVRALWTREYTPQLSAEFKNAVSK
YGSQFQGNSQHDALEFLLWLLDRVHEDLEGSSRGPVSEKLPPEATKTSEN
CLSPSAQLPLGQSFVQSHFQAQYRSSLTCPHCLKQSNTFDPFLCVSLPIP
LRQTRFLSVTLVFPSKSQRFLRVGLAVPILSTVAALRKMVAEEGGVPADE
VILVELYPSGFQRSFFDEEDLNTIAEGDNVYAFQVPPSPSQGTLSAHPLG
LSASPRLAAREGQRFSLSLHSESKVLILFCNLVGSGQQASRFGPPFLIRE
DRAVSWAQLQQSILSKVRHLMKSEAPVQNLGSLFSIRVVGLSVACSYLSP
KDSRPLCHWAVDRVLHLRRPGGPPHVKLAVEWDSSVKERLFGSLQEERAQ
DADSVWQQQQAHQQHSCTLDECFQFYTKEEQLAQDDAWKCPHCQVLQQGM
VKLSLWTLPDILIIHLKRFCQVGERRNKLSTLVKFPLSGLNMAPHVAQRS
TSPEAGLGPWPSWKQPDCLPTSYPLDFLYDLYAVCNHHGNLQGGHYTAYC
RNSLDGQWYSYDDSTVEPLREDEVNTRGAYILFYQKRNSIPPWSASSSMR
GSTSSSLSDHWLLRLGSHAGSTRGSLLSWSSAPCPSLPQVPDSPIFTNSL
CNQEKGGLEPRRLVRGVKGRSISMKAPTTSRAKQGPFKTMPLRWSFGSKE
KPPGASVELVEYLESRRRPRSTSQSIVSLLTGTAGEDEKSASPRSNVALP
ANSEDGGRAIERGPAGVPCPSAQPNHCLAPGNSDGPNTARKLKENAGQDI
KLPRKFDLPLTVMPSVEHEKPARPEGQKAMNWKESFQMGSKSSPPSPYMG
FSGNSKDSRRGTSELDRPLQGTLTLLRSVFRKKENRRNERAEVSPQVPPV
SLVSGGLSPAMDGQAPGSPPALRIPEGLARGLGSRLERDVWSAPSSLRLP
RKASRAPRGSALGMSQRTVPGEQASYGTFQRVKYHTLSLGRKKTLPESSF
AAAYPYDVPDYA*
Vector Type
Mammalian
Antibiotic
Amp
100042 R 1000 1001 1047 19007
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