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Plasmid
pcDNA5-FRT/TO-EYFP-PIAS4 (siRNA resistant) deletion SIM
Parent Plasmid
pcDNA5 FRT/TO
Expressed
EYFP-PIAS4 (siRNA resistant) delta A463-S472
DU Number
DU24571
Genbank
BC029874 NM_015897.2
Tag
YFP
Species
Human
Sequence of Insert
View Sequence
ggatccAAGATGGCGGCGGAGCTGGTGGAGGCCAAAAACATGGTGATGAG
TTTTCGAGTCTCCGACCTTCAGATGCTCCTGGGTTTCGTGGGCCGGAGTA
AGAGTGGACTGAAGCACGAGCTCGTCACCAGGGCCCTCCAGCTGGTGCAG
TTTGACTGTAGCCCTGAGCTGTTCAAGAAGATCAAGGAGCTGTACGAGAC
CCGCTACGCCAAGAAGAACTCGGAGCCTGCCCCACAGCCGCACCGGCCCC
TGGACCCCCTGACCATGCACTCCACCTACGACCGGGCCGGCGCTGTGCCC
AGGACTCCGCTGGCAGGCCCCAATATTGACTACCCCGTGCTCTACGGAAA
GTACTTAAACGGACTGGGACGGTTGCCCGCCAAGACCCTCAAGCCAGAAG
TCCGCCTGGTGAAGCTGCCGTTCTTTAATATGCTGGATGAGCTGCTGAAG
CCCACCGAAttggttcctCAGAACAACGAGAAGCTTCAGGAGAGCCCGTG
CATCTTCGCATTGACGCCAAGACAGGTGGAGTTGATCCGGAACTCCAGGG
AACTGCAGCCCGGAGTTAAAGCCGTGCAGGTCGTCCTGAGAATCTGTTAC
TCAGACACCAGCTGCCCTCAGGAGGACCAGTACCCGCCCAACATCGCTGT
GAAGGTCAACCACAGCTACTGCTCCGTCCCGGGCTACTACCCCTCCAATA
AGCCCGGGGTGGAGCCCAAGAGGCCGTGCCGCCCCATCAACCTCACTCAC
CTCATGTACCTGTCCTCGGCCACCAACCGCATCACTGTCACCTGGGGGAA
CtatggtaaaAGCTACTCGGTGGCCCTGTACCTGGTGCGGCAGCTGACCT
CATCGGAGCTGCTGCAGAGGCTGAAGACCATTGGGGTAAAGCACCCGGAG
CTGTGCAAGGCACTGGTCAAGGAGAAGCTGCGCCTTGATCCTGACAGCGA
GATCGCCACCACCGGTGTGCGGGTGTCCCTCATCTGTCCGCTGGTGAAGA
TGCGGCTCTCCGTGCCCTGCCGGGCAGAGACCTGCGCCCACCTGCAGTGC
TTCGACGCCGTCTTCTACCTGCAGATGAACGAGAAGAAGCCCACCTGGAT
GTGCCCCGTGTGCGACAAGCCAGCCCCCTACGACCAGCTCATCATCGACG
GGCTCCTCTCGAAGATCCTGAGCGAGTGTGAGGACGCCGACGAGATCGAG
TACCTGGTGGACGGCTCGTGGTGCCCGATCCGCGCCGAAAAGGAGCGCAG
CTGCAGCCCGCAGGGCGCCATCCTCGTGCTGGGCCCCTCGGACGCCAATG
GGCTCCTGCCCGCCCCCAGCGTCAACGGGAGCGGTGCCCTGGGCAGCACG
GGTGGCGGCGGCCCGGTGGGCAGCATGGAGAATGGGAAGCCGGGCTCATC
GTCCTCGGAGGATGAGGAGGAGGAGGAAGAGGAGGAGGAAGACGAGGACG
AAGAGGGGCCCCGGCCCAAGCGCCGCTGCCCCTTCCAGAAGGGCCTGGTG
CCGGCCTGCTGACCCCGGCCGCACACTCGACgcggccgc
TTTTCGAGTCTCCGACCTTCAGATGCTCCTGGGTTTCGTGGGCCGGAGTA
AGAGTGGACTGAAGCACGAGCTCGTCACCAGGGCCCTCCAGCTGGTGCAG
TTTGACTGTAGCCCTGAGCTGTTCAAGAAGATCAAGGAGCTGTACGAGAC
CCGCTACGCCAAGAAGAACTCGGAGCCTGCCCCACAGCCGCACCGGCCCC
TGGACCCCCTGACCATGCACTCCACCTACGACCGGGCCGGCGCTGTGCCC
AGGACTCCGCTGGCAGGCCCCAATATTGACTACCCCGTGCTCTACGGAAA
GTACTTAAACGGACTGGGACGGTTGCCCGCCAAGACCCTCAAGCCAGAAG
TCCGCCTGGTGAAGCTGCCGTTCTTTAATATGCTGGATGAGCTGCTGAAG
CCCACCGAAttggttcctCAGAACAACGAGAAGCTTCAGGAGAGCCCGTG
CATCTTCGCATTGACGCCAAGACAGGTGGAGTTGATCCGGAACTCCAGGG
AACTGCAGCCCGGAGTTAAAGCCGTGCAGGTCGTCCTGAGAATCTGTTAC
TCAGACACCAGCTGCCCTCAGGAGGACCAGTACCCGCCCAACATCGCTGT
GAAGGTCAACCACAGCTACTGCTCCGTCCCGGGCTACTACCCCTCCAATA
AGCCCGGGGTGGAGCCCAAGAGGCCGTGCCGCCCCATCAACCTCACTCAC
CTCATGTACCTGTCCTCGGCCACCAACCGCATCACTGTCACCTGGGGGAA
CtatggtaaaAGCTACTCGGTGGCCCTGTACCTGGTGCGGCAGCTGACCT
CATCGGAGCTGCTGCAGAGGCTGAAGACCATTGGGGTAAAGCACCCGGAG
CTGTGCAAGGCACTGGTCAAGGAGAAGCTGCGCCTTGATCCTGACAGCGA
GATCGCCACCACCGGTGTGCGGGTGTCCCTCATCTGTCCGCTGGTGAAGA
TGCGGCTCTCCGTGCCCTGCCGGGCAGAGACCTGCGCCCACCTGCAGTGC
TTCGACGCCGTCTTCTACCTGCAGATGAACGAGAAGAAGCCCACCTGGAT
GTGCCCCGTGTGCGACAAGCCAGCCCCCTACGACCAGCTCATCATCGACG
GGCTCCTCTCGAAGATCCTGAGCGAGTGTGAGGACGCCGACGAGATCGAG
TACCTGGTGGACGGCTCGTGGTGCCCGATCCGCGCCGAAAAGGAGCGCAG
CTGCAGCCCGCAGGGCGCCATCCTCGTGCTGGGCCCCTCGGACGCCAATG
GGCTCCTGCCCGCCCCCAGCGTCAACGGGAGCGGTGCCCTGGGCAGCACG
GGTGGCGGCGGCCCGGTGGGCAGCATGGAGAATGGGAAGCCGGGCTCATC
GTCCTCGGAGGATGAGGAGGAGGAGGAAGAGGAGGAGGAAGACGAGGACG
AAGAGGGGCCCCGGCCCAAGCGCCGCTGCCCCTTCCAGAAGGGCCTGGTG
CCGGCCTGCTGACCCCGGCCGCACACTCGACgcggccgc
Amino Acid Sequence
View Sequence
MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICT
TGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
VYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSYQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKGSKMAAELVEA
KNMVMSFRVSDLQMLLGFVGRSKSGLKHELVTRALQLVQFDCSPELFKKI
KELYETRYAKKNSEPAPQPHRPLDPLTMHSTYDRAGAVPRTPLAGPNIDY
PVLYGKYLNGLGRLPAKTLKPEVRLVKLPFFNMLDELLKPTELVPQNNEK
LQESPCIFALTPRQVELIRNSRELQPGVKAVQVVLRICYSDTSCPQEDQY
PPNIAVKVNHSYCSVPGYYPSNKPGVEPKRPCRPINLTHLMYLSSATNRI
TVTWGNYGKSYSVALYLVRQLTSSELLQRLKTIGVKHPELCKALVKEKLR
LDPDSEIATTGVRVSLICPLVKMRLSVPCRAETCAHLQCFDAVFYLQMNE
KKPTWMCPVCDKPAPYDQLIIDGLLSKILSECEDADEIEYLVDGSWCPIR
AEKERSCSPQGAILVLGPSDANGLLPAPSVNGSGALGSTGGGGPVGSMEN
GKPGSSSSEDEEEEEEEEEDEDEEGPRPKRRCPFQKGLVPAC*
TGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
VYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSYQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKGSKMAAELVEA
KNMVMSFRVSDLQMLLGFVGRSKSGLKHELVTRALQLVQFDCSPELFKKI
KELYETRYAKKNSEPAPQPHRPLDPLTMHSTYDRAGAVPRTPLAGPNIDY
PVLYGKYLNGLGRLPAKTLKPEVRLVKLPFFNMLDELLKPTELVPQNNEK
LQESPCIFALTPRQVELIRNSRELQPGVKAVQVVLRICYSDTSCPQEDQY
PPNIAVKVNHSYCSVPGYYPSNKPGVEPKRPCRPINLTHLMYLSSATNRI
TVTWGNYGKSYSVALYLVRQLTSSELLQRLKTIGVKHPELCKALVKEKLR
LDPDSEIATTGVRVSLICPLVKMRLSVPCRAETCAHLQCFDAVFYLQMNE
KKPTWMCPVCDKPAPYDQLIIDGLLSKILSECEDADEIEYLVDGSWCPIR
AEKERSCSPQGAILVLGPSDANGLLPAPSVNGSGALGSTGGGGPVGSMEN
GKPGSSSSEDEEEEEEEEEDEDEEGPRPKRRCPFQKGLVPAC*
Vector Type
Mammalian
Antibiotic
Amp
Comments
Silent mutations at L151 V152 P153 (tta gtc cca → ttg gtt cct ) and Y265 G266 K267 (tac ggc aag → tat ggt aaa) plus SIM mutation (delta A463-S472). clone has an additional N-terminal lysine upstream from PIAS4 initiation. ORF stop located approx 20bp upstream of 3' Not1 cloning site.
100042 R 1000 1001 1047 19007
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