PIAS1 | Ubiquitylation Resource

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Synonyms

protein inhibitor of activated STAT 1, GBP, Ddxbp1, 2900068C24Rik

Plasmid

pcDNA5-FRT/TO-EYFP-PIAS1 (siRNA resistant) deletion SIM

Parent Plasmid

pcDNA5 FRT/TO

Expressed

EYFP-PIAS1 (siRNA resistant) delta V457-S466

DU Number

DU24569

Genbank

BAC35902 Gene ID: 56469

Tag

YFP

Species

Mouse

Sequence of Insert

View Sequence

ggatccATGGCGGACAGTGCGGAACTAAAGCAAATGGTTATGAGCCTTAGAGTTTC
TGAACTCCAAGTACTGTTGGGCTACGCTGGGAGGAACAAGCACGGACGCA
AACACGAACTTCTTACAAAAGCCCTGCATTTGTTAAAGGCTGGCTGTAGT
CCTGCTGTACAAATGAAAATTAAAGAACTCTACAGGAGGCGGTTCCCTCA
GAAAATTATGACGCCTGCGGACTTGTCTATCCCCAACGTACATTCAAGTC
CTATGCCTCCGACTCTTTCTCCATCCACCATTCCACAGCTCACTTATGAT
GGCCACCCTGCATCATCCCCACTACTCCCTGTTTCTCTTCTGGGACCCAA
ACATGAACTGGAACTCCCACATCTCACGTCAGCGCTGCACCCAGTCCACC
CGGACATAAAGCTGCAGAAGCTACCATTCTATGACCTGTTGGATGAACTG
ATCAAGCCCACCAGTCTAGCTTCAGACAACAGCCAGCGCTTTCGGGAAAC
CTGTTTTGCATTTGCCTTGACACCACAACAGGTGCAGCAGATCAGCAGCT
CCATGGATATTTCTGGGACCAAATGTGACTTCACAGTGCAGGTCCAATTA
AGGTTTTGTTTATCAGAAACCAGTTGTCCACAAGAAGATCACTTCCCACC
CAACCTTTGTGTAAAAGTGAATACAAAACCTTGCAGCCTTCCAGGTTACC
TTCCACCTACTAAAAACGGTGTGGAACCAAAGCGACCTagtcgtcctATT
AATATCACCTCACTTGTCCGATTGTCCACGACAGTACCAAATACCATTGT
TGTTTCTTGGACTGCAGAAATTGGAAGAACCTATTCCATGGCAGTATATC
TTGTAAAACAGTTGTCCTCAACAGTTCTTCTTCAGAGGTTACGAGCAAAG
GGAATAagaaacccaGATCATTCTAGAGCTTTAATTAAAGAGAAGTTAAC
TGCAGATCCAGATAGTGAGATAGCTACTACCAGCCTACGGGTTTCGCTGC
TGTGTCCACTTGGGAAAATGCGACTGACAATCCCCTGTCGGGCACTTACC
TGCTCCCACCTTCAGTGTTTTGATGCAACTCTTTACATTCAAATGAATGA
GAAAAAACCAACATGGGTTTGTCCTGTCTGTGATAAGAAGGCCCCATATG
AACACCTTATTATTGACGGGTTGTTTATGGAAATTCTAAAGTACTGCACA
GACTGTGACGAGATACAGTTTAAGGAGGATGGCTCGTGGGCTCCAATGAG
GTCAAAGAAGGAGGTTCAAGAAGTCACTGCCTCCTACAATGGAGTTGATG
GTTGCTTGAGCTCCACATTGGAGCATCAGGTAGCGTCCCACAACCAGTCC
TCAAATAAAAACAAGAAATCGTCAGATGAAGAGGAGGAAGAACCCCCTGC
CAAGAGGACCTGTCCTTCCCTGTCTCCTACGTCACCACTAAGTAATAAAG
GCATTTTAAGTCTTCCTCATCAAGCCTCGCCTGTGTCCCGCACCCCAAGC
CTTCCTGCTGTAGATACAAGCTACATCAACACCTCCCTCATCCAGGACTA
CAGGCACCCCTTCCACATGACGCCTATGCCTTATGACTTACAAGGATTAG
ATTTCTTTCCTTTCTTATCAGGAGACAATCAGCATTACAACACCTCCCTG
CTAGCCGCAGCTGCAGCGGCGGTCTCAGATGACCAGGACCTCCTGCACTC
CTCCCGGTTTTTCCCGTATACCTCCTCGCAGATGTTTCTCGACCAGCTAA
GTGCAGGAGGGAGCACATCTCTGCCAGCCACCAACGGAAGCAGTAGCGGC
AGCAACAGCAGCCTTGTGTCTTCCAACAGTCTGAGAGAGAGCCATGGCCA
TGGTGTGGCCAGCAGGAGCAGCGCAGACACAGCGTCCATCTTTGGCATCA
TACCAGACATTATCTCATTGGACTGAGCGGCCGC

Amino Acid Sequence

View Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICT
TGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
VYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSYQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKGSMADSAELKQ
MVMSLRVSELQVLLGYAGRNKHGRKHELLTKALHLLKAGCSPAVQMKIKE
LYRRRFPQKIMTPADLSIPNVHSSPMPPTLSPSTIPQLTYDGHPASSPLL
PVSLLGPKHELELPHLTSALHPVHPDIKLQKLPFYDLLDELIKPTSLASD
NSQRFRETCFAFALTPQQVQQISSSMDISGTKCDFTVQVQLRFCLSETSC
PQEDHFPPNLCVKVNTKPCSLPGYLPPTKNGVEPKRPSRPINITSLVRLS
TTVPNTIVVSWTAEIGRTYSMAVYLVKQLSSTVLLQRLRAKGIRNPDHSR
ALIKEKLTADPDSEIATTSLRVSLLCPLGKMRLTIPCRALTCSHLQCFDA
TLYIQMNEKKPTWVCPVCDKKAPYEHLIIDGLFMEILKYCTDCDEIQFKE
DGSWAPMRSKKEVQEVTASYNGVDGCLSSTLEHQVASHNQSSNKNKKSSD
EEEEEPPAKRTCPSLSPTSPLSNKGILSLPHQASPVSRTPSLPAVDTSYI
NTSLIQDYRHPFHMTPMPYDLQGLDFFPFLSGDNQHYNTSLLAAAAAAVS
DDQDLLHSSRFFPYTSSQMFLDQLSAGGSTSLPATNGSSSGSNSSLVSSN
SLRESHGHGVASRSSADTASIFGIIPDIISLD*

Vector Type

Mammalian

Antibiotic

Amp

Comments

Silent mutations at S247 R248 P249 (agc cga cca → agt cgt cct ) and R303 N304 P305 (agg aat ccg → aga aac cca) plus SIM mutation (delta V457-S466)

100042 R 1000 1001 1047 19007

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