This reagent and more can be found at our sister site MRC PPU Reagents & Services
Request Now
Plasmid
pcDNA FRT TO GFP USP43
Parent Plasmid
pcDNA5D FRT/TO GFP
Expressed
GFP-USP43
DU Number
DU47093
Genbank
NM_001267576.1
Tag
GFP
Species
Human
Sequence of Insert
View Sequence
GTCGACATGGACCTGGGCCCCGGGGACGCGGCAGGAGGGGGACCGCTCGCGCCCCGGCCCCGCCGCCGCCGCTCCCTGCGCCGCCTGTTCAGCCGCTTCCTGCTGGCGCTGGGCAGCCGCTCACGCCCCGGGGACTCACCGCCCCGGCCCCAGCCGGGACACTGTGATGGCGACGGTGAGGGGGGCTTCGCCTGCGCCCCGGGCCCAGTTCCAGCGGCCCCCGGGAGCCCCGGGGAGGAACGCCCGCCCGGACCCCAGCCCCAGCTCCAGCTCCCCGCCGGCGATGGGGCGCGGCCGCCGGGCGCTCAGGGCTTGAAGAACCACGGCAACACCTGTTTCATGAACGCGGTGGTGCAGTGTCTCAGCAACACCGACCTGCTGGCCGAGTTCCTGGCGCTGGGGCGCTACCGGGCGGCTCCGGGCCGCGCCGAGGTCACCGAGCAGCTGGCGGCGCTGGTGCGCGCGCTCTGGACTCGCGAATACACGCCCCAACTTTCCGCGGAGTTCAAGAATGCAGTTTCCAAGTACGGCTCTCAGTTCCAAGGCAATTCCCAGCACGACGCCCTGGAATTCCTGCTCTGGTTGCTGGATCGTGTACATGAGGACCTGGAGGGTTCATCCCGAGGGCCGGTGTCGGAGAAGCTTCCGCCTGAAGCCACTAAAACCTCTGAGAACTGCCTGTCACCATCAGCTCAGCTTCCTCTAGGTCAAAGCTTTGTGCAAAGCCACTTTCAAGCACAATATAGATCTTCCTTGACTTGTCCCCACTGCCTGAAACAGAGCAACACCTTTGATCCTTTCCTGTGTGTGTCCCTACCTATCCCCTTGCGCCAGACGAGGTTCTTGAGTGTCACCTTGGTCTTCCCCTCTAAGAGCCAGCGGTTCCTGCGGGTTGGCCTGGCCGTGCCGATCCTCAGCACAGTGGCAGCCCTGAGGAAGATGGTTGCAGAGGAAGGAGGCGTCCCTGCAGATGAGGTGATCTTGGTTGAACTGTATCCCAGTGGATTCCAGCGGTCTTTCTTTGATGAAGAGGACCTGAATACCATCGCAGAGGGAGATAATGTGTATGCCTTTCAAGTTCCTCCCTCACCCAGCCAGGGGACTCTCTCAGCTCATCCACTGGGTCTGTCGGCCTCCCCACGCCTGGCAGCCCGTGAGGGCCAGCGATTCTCCCTCTCTCTCCACAGTGAGAGCAAGGTGCTAATCCTCTTCTGTAACTTGGTGGGGTCAGGGCAGCAGGCTAGCAGGTTTGGGCCACCCTTCCTGATAAGGGAAGACAGAGCTGTTTCCTGGGCCCAGCTCCAGCAGTCTATCCTCAGCAAGGTCCGCCATCTTATGAAGAGTGAGGCCCCTGTACAGAACCTGGGGTCTCTGTTCTCCATCCGTGTTGTGGGACTCTCTGTGGCCTGCAGCTATTTGTCTCCGAAGGACAGTCGGCCCCTCTGTCACTGGGCAGTTGACAGGGTTTTGCATCTCAGGAGGCCAGGAGGCCCTCCACATGTCAAGCTGGCGGTGGAGTGGGATAGCTCTGTCAAGGAGCGCCTGTTCGGGAGCCTCCAGGAGGAGCGAGCGCAGGATGCCGACAGTGTGTGGCAGCAGCAGCAGGCGCATCAGCAGCACAGCTGTACCTTGGATGAATGTTTTCAGTTCTACACCAAGGAGGAGCAGCTGGCCCAGGATGACGCCTGGAAGTGTCCTCACTGCCAAGTCCTGCAGCAGGGGATGGTGAAGCTGAGTTTGTGGACGCTGCCTGACATCCTCATCATCCACCTCAAAAGGTTCTGCCAGGTGGGCGAGAGAAGAAACAAGCTCTCCACGCTGGTGAAGTTTCCGCTCTCTGGACTCAACATGGCTCCCCATGTGGCCCAGAGAAGCACCAGCCCTGAGGCAGGACTGGGCCCCTGGCCTTCCTGGAAGCAGCCGGACTGCCTGCCCACCAGTTACCCGCTGGACTTCCTGTACGACCTGTATGCCGTCTGCAACCACCATGGCAACCTGCAAGGTGGGCATTACACAGCCTACTGCCGGAACTCTCTGGATGGCCAGTGGTACAGTTATGATGACAGCACGGTGGAACCGCTTCGAGAAGATGAGGTCAACACCAGAGGGGCTTATATCCTGTTCTATCAGAAGCGGAACAGCATCCCTCCCTGGTCAGCCAGCAGCTCCATGAGAGGCTCTACCAGCTCCTCCCTGTCTGATCACTGGCTCTTACGGCTCGGGAGCCACGCTGGCAGCACAAGGGGAAGCCTGCTGTCCTGGAGCTCTGCCCCCTGCCCCTCCCTGCCCCAGGTTCCTGACTCTCCCATCTTCACCAACAGCCTCTGCAATCAGGAAAAGGGAGGGTTGGAGCCCAGGCGTTTGGTACGGGGCGTGAAAGGCAGAAGCATTAGCATGAAGGCACCCACCACTTCCCGAGCCAAGCAGGGACCATTCAAGACCATGCCTCTGCGGTGGTCCTTTGGATCCAAGGAGAAACCACCAGGTGCCTCCGTCGAGTTGGTGGAGTACTTGGAATCCAGACGAAGACCTCGGTCCACGAGCCAGTCCATTGTGTCGCTGTTGACGGGCACTGCGGGTGAGGATGAGAAGTCAGCATCGCCGAGGTCCAACGTCGCCCTTCCTGCTAACAGCGAAGATGGTGGGCGGGCCATTGAAAGAGGTCCAGCCGGGGTGCCCTGTCCCTCGGCTCAACCCAACCACTGTCTGGCCCCTGGAAACTCAGATGGTCCAAACACAGCAAGGAAACTCAAGGAAAATGCAGGGCAGGACATCAAGCTTCCCAGAAAGTTTGACCTGCCTCTCACTGTGATGCCTTCAGTGGAGCATGAGAAACCAGCTCGACCGGAGGGCCAGAAGGCCATGAACTGGAAGGAGAGCTTCCAGATGGGAAGCAAAAGCAGCCCACCCTCCCCCTATATGGGATTCTCTGGAAACAGCAAAGACAGTCGCCGAGGCACCTCTGAGCTAGACAGACCCCTGCAGGGGACACTCACCCTTCTGAGGTCCGTGTTTCGGAAGAAGGAGAACAGGAGGAATGAGAGGGCAGAGGTCTCTCCACAGGTGCCCCCCGTCTCCCTGGTGAGTGGCGGGCTGAGCCCTGCCATGGACGGGCAGGCTCCAGGCTCACCTCCTGCCCTCAGGATCCCAGAGGGCCTGGCCAGGGGCCTGGGCAGCCGGCTCGAGAGGGATGTCTGGTCAGCCCCCAGCTCTCTCCGCCTCCCTCGTAAAGCCAGCAGGGCCCCGAGAGGCAGTGCACTGGGCATGTCACAAAGGACTGTTCCAGGGGAGCAGGCTTCTTATGGCACCTTTCAGAGAGTCAAATATCACACTCTTTCTTTAGGTCGAAAGAAAACCTTACCGGAGTCCAGCTTTTGAGTCGAC
Amino Acid Sequence
View Sequence
MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICT
TGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
VYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLGSPGEFAS
LDMDLGPGDAAGGGPLAPRPRRRRSLRRLFSRFLLALGSRSRPGDSPPRP
QPGHCDGDGEGGFACAPGPVPAAPGSPGEERPPGPQPQLQLPAGDGARPP
GAQGLKNHGNTCFMNAVVQCLSNTDLLAEFLALGRYRAAPGRAEVTEQLA
ALVRALWTREYTPQLSAEFKNAVSKYGSQFQGNSQHDALEFLLWLLDRVH
EDLEGSSRGPVSEKLPPEATKTSENCLSPSAQLPLGQSFVQSHFQAQYRS
SLTCPHCLKQSNTFDPFLCVSLPIPLRQTRFLSVTLVFPSKSQRFLRVGL
AVPILSTVAALRKMVAEEGGVPADEVILVELYPSGFQRSFFDEEDLNTIA
EGDNVYAFQVPPSPSQGTLSAHPLGLSASPRLAAREGQRFSLSLHSESKV
LILFCNLVGSGQQASRFGPPFLIREDRAVSWAQLQQSILSKVRHLMKSEA
PVQNLGSLFSIRVVGLSVACSYLSPKDSRPLCHWAVDRVLHLRRPGGPPH
VKLAVEWDSSVKERLFGSLQEERAQDADSVWQQQQAHQQHSCTLDECFQF
YTKEEQLAQDDAWKCPHCQVLQQGMVKLSLWTLPDILIIHLKRFCQVGER
RNKLSTLVKFPLSGLNMAPHVAQRSTSPEAGLGPWPSWKQPDCLPTSYPL
DFLYDLYAVCNHHGNLQGGHYTAYCRNSLDGQWYSYDDSTVEPLREDEVN
TRGAYILFYQKRNSIPPWSASSSMRGSTSSSLSDHWLLRLGSHAGSTRGS
LLSWSSAPCPSLPQVPDSPIFTNSLCNQEKGGLEPRRLVRGVKGRSISMK
APTTSRAKQGPFKTMPLRWSFGSKEKPPGASVELVEYLESRRRPRSTSQS
IVSLLTGTAGEDEKSASPRSNVALPANSEDGGRAIERGPAGVPCPSAQPN
HCLAPGNSDGPNTARKLKENAGQDIKLPRKFDLPLTVMPSVEHEKPARPE
GQKAMNWKESFQMGSKSSPPSPYMGFSGNSKDSRRGTSELDRPLQGTLTL
LRSVFRKKENRRNERAEVSPQVPPVSLVSGGLSPAMDGQAPGSPPALRIP
EGLARGLGSRLERDVWSAPSSLRLPRKASRAPRGSALGMSQRTVPGEQAS
YGTFQRVKYHTLSLGRKKTLPESSF*
TGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
VYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLGSPGEFAS
LDMDLGPGDAAGGGPLAPRPRRRRSLRRLFSRFLLALGSRSRPGDSPPRP
QPGHCDGDGEGGFACAPGPVPAAPGSPGEERPPGPQPQLQLPAGDGARPP
GAQGLKNHGNTCFMNAVVQCLSNTDLLAEFLALGRYRAAPGRAEVTEQLA
ALVRALWTREYTPQLSAEFKNAVSKYGSQFQGNSQHDALEFLLWLLDRVH
EDLEGSSRGPVSEKLPPEATKTSENCLSPSAQLPLGQSFVQSHFQAQYRS
SLTCPHCLKQSNTFDPFLCVSLPIPLRQTRFLSVTLVFPSKSQRFLRVGL
AVPILSTVAALRKMVAEEGGVPADEVILVELYPSGFQRSFFDEEDLNTIA
EGDNVYAFQVPPSPSQGTLSAHPLGLSASPRLAAREGQRFSLSLHSESKV
LILFCNLVGSGQQASRFGPPFLIREDRAVSWAQLQQSILSKVRHLMKSEA
PVQNLGSLFSIRVVGLSVACSYLSPKDSRPLCHWAVDRVLHLRRPGGPPH
VKLAVEWDSSVKERLFGSLQEERAQDADSVWQQQQAHQQHSCTLDECFQF
YTKEEQLAQDDAWKCPHCQVLQQGMVKLSLWTLPDILIIHLKRFCQVGER
RNKLSTLVKFPLSGLNMAPHVAQRSTSPEAGLGPWPSWKQPDCLPTSYPL
DFLYDLYAVCNHHGNLQGGHYTAYCRNSLDGQWYSYDDSTVEPLREDEVN
TRGAYILFYQKRNSIPPWSASSSMRGSTSSSLSDHWLLRLGSHAGSTRGS
LLSWSSAPCPSLPQVPDSPIFTNSLCNQEKGGLEPRRLVRGVKGRSISMK
APTTSRAKQGPFKTMPLRWSFGSKEKPPGASVELVEYLESRRRPRSTSQS
IVSLLTGTAGEDEKSASPRSNVALPANSEDGGRAIERGPAGVPCPSAQPN
HCLAPGNSDGPNTARKLKENAGQDIKLPRKFDLPLTVMPSVEHEKPARPE
GQKAMNWKESFQMGSKSSPPSPYMGFSGNSKDSRRGTSELDRPLQGTLTL
LRSVFRKKENRRNERAEVSPQVPPVSLVSGGLSPAMDGQAPGSPPALRIP
EGLARGLGSRLERDVWSAPSSLRLPRKASRAPRGSALGMSQRTVPGEQAS
YGTFQRVKYHTLSLGRKKTLPESSF*
Vector Type
Mammalian
Antibiotic
Amp
Comments
N-terminal GFP tag on USP43 in pcDNA5 FRT/TO, please. Thanks!
100042 R 1000 1001 1047 19007
Download datasheet