Ubiquitin

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Plasmid
pET24a 6His Ubiquitin S65D G75A G76A
Parent Plasmid
pET24a original
Expressed
6His-Ubiquitin S65D G75A G76A
DU Number
DU49520
Genbank
P62988*
Tag
6His
Species
Human
Sequence of Insert
ATGCATCACCATCACCATCACGGCGGTATGCAGATCTTCGTGAAGACCCTGACTGGTAAGACCATCACTCTCGAAGTGGAGCCGAGTGACACCATTGAGAATGTCAAGGCAAAGATCCAAGACAAGGAAGGCATCCCTCCTGACCAGCAGAGGTTGATCTTTGCTGGGAAACAGCTGGAAGATGGACGCACCCTGTCTGACTACAACATCCAGAAAGAGGACACCCTGCACCTGGTCCTCCGTCTCCGAGCTGCCTGATAAGGATCC

Amino Acid Sequence
MHHHHHHGGMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQ
RLIFAGKQLEDGRTLSDYNIQKEDTLHLVLRLRAA*
Vector Type
Bacterial
Antibiotic
Kan
Comments
*Differs from reference sequence in having silent mutation at Arg73 (a220c substitution conferred by reverse primer 54389).



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