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Ubiquitin

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Plasmid
pET24-Ubiquitin G75A G76A
Parent Plasmid
pET24a original
Expressed
untagged Ubiquitin G75A G76A
DU Number
DU49488
Genbank
P62988*
Tag
untagged
Species
Human
Sequence of Insert
CATATGCAGATCTTCGTGAAGACCCTGACTGGTAAGACCATCACTCTCGAAGTGGAGCCGAGTGACACCATTGAGAATGTCAAGGCAAAGATCCAAGACAAGGAAGGCATCCCTCCTGACCAGCAGAGGTTGATCTTTGCTGGGAAACAGCTGGAAGATGGACGCACCCTGTCTGACTACAACATCCAGAAAGAGTCCACCCTGCACCTGGTCCTCCGTCTCCGAGCTGCCTGATAAGGATCC

Amino Acid Sequence
MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQL
EDGRTLSDYNIQKESTLHLVLRLRAA**
Vector Type
Bacterial
Antibiotic
Kan
Comments
*Bacterial expression construct for untagged ubiquitin (Arg73 codon AGA mutated to more common CGA)
Differs from reference sequence in having silent mutation at Arg73 (a220c substitution conferred by reverse primer 54389).
T7 tag in pET24a removed by subcloning into the Nde1 site, to give untagged protein.

Estimated MW of expressed protein : 8565 Daltons
100042 R 1000 1001 1047 19007
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