Ubiquitin

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Plasmid
pGEX6P1-Ubiquitin trimer
Parent Plasmid
pGEX6P1
Expressed
GST-C3-Ubiquitin trimer
DU Number
DU20730
Genbank
P62988
Tag
GST
Species
Human
Sequence of Insert
GCCGGCATGCAGATCTTCGTGAAGACCCTGACTGGTAAGACCATCACTCT
CGAAGTGGAGCCGAGTGACACCATTGAGAATGTCAAGGCAAAGATCCAAG
ACAAGGAAGGCATCCCTCCTGACCAGCAGAGGTTGATCTTTGCTGGGAAA
CAGCTGGAAGATGGACGCACCCTGTCTGACTACAACATCCAGAAAGAGTC
CACCCTGCACCTGGTCCTCCGTCTCAGAGGCGCC

Amino Acid Sequence
MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSAGMQIFVKTLTGKTITLEV
EPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTL
HLVLRLRGGMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQ
RLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITL
EVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKES
TLHLVLRLRGG**
Vector Type
Bacterial
Antibiotic
Amp; Kan
Comments
GST-ubiquitin (wild type) trimer and intermediate in the assembly of wild type polyubiquitin chains

Estimated MW of expressed protein : 52611 Daltons
100042 R 1000 1001 1047 19007
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