1. Home
  3. Ubiquitin

Ubiquitin

This reagent and more can be found at our sister site MRC PPU Reagents & Services
Request Now
Plasmid
pEGFPC4-Ubiquitin G75A G76A dimer
Parent Plasmid
pEGFPC4
Expressed
eGFP-Ubiquitin G75A G76A dimer
DU Number
DU20439
Genbank
P62988*
Tag
GFP
Species
Human
Sequence of Insert
GGATCCGGCGCCATGCAGATCTTCGTGAAGACCCTGACTGGTAAGACCATCACTCTCGAAGTGGAGCCGAGTGACACCATTGAGAATGTCAAGGCAAAGATCCAAGACAAGGAAGGCATCCCTCCTGACCAGCAGAGGTTGATCTTTGCTGGGAAACAGCTGGAAGATGGACGCACCCTGTCTGACTACAACATCCAGAAAGAGTCCACCCTGCACCTGGTCCTCCGTCTCAGAGCCGCCATGCAGATCTTCGTGAAGACCCTGACTGGTAAGACCATCACTCTCGAAGTGGAGCCGAGTGACACCATTGAGAATGTCAAGGCAAAGATCCAAGACAAGGAAGGCATCCCTCCTGACCAGCAGAGGTTGATCTTTGCTGGGAAACAGCTGGAAGATGGACGCACCCTGTCTGACTACAACATCCAGAAAGAGTCCACCCTGCACCTGGTCCTCCGTCTCAGAGCTGCTTGATAAGCGGCCGC
Amino Acid Sequence
MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICT
TGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
VYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLRSPGSGAM
QIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLE
DGRTLSDYNIQKESTLHLVLRLRAAMQIFVKTLTGKTITLEVEPSDTIEN
VKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRA
A**
Vector Type
Mammalian
Antibiotic
Kan
Comments
*Each unit differs from ref sequence in having C-terminal AA instead of GG. BamHI-NotI flanked dimer plus STOP codon.
Plasmid for mammalian expression of N-terminally eGFP tagged diubiquitin
Parental Plasmid DU32794
Estimated MW of expressed protein : 44963 Daltons
100042 R 1000 1001 1047 19007
Download datasheet