Ubiquitin | Ubiquitylation Resource

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Plasmid

pcDNA5-FRT/TO-GS Ubiquitin KallR

Parent Plasmid

pcDNA5 FRT/TO

Expressed

GS-Ubiquitin KallR

DU Number

DU20502

Genbank

P62988

Species

Human

Sequence of Insert

View Sequence

AAGCTTGCCACCATGGGCACCCCCGCAGTCACCACCTACAAGCTGGTCAT
TAACGGCAAGACTCTGAAGGGCGAGACCACCACCAAGGCCGTGGACGCAG
AAACCGCGGAAAAGGCGTTCAAGCAGTACGCGAACGACAACGGGGTGGAC
GGAGTCTGGACCTACGATGACGCTACAAAAACGTTCACCGTGACCGAGGT
TAACACCCCCGCTGTGACTACGTACAAGCTGGTGATCAATGGGAAGACCC
TGAAGGGCGAGACCACCACGAAAGCTGTAGACGCCGAGACAGCCGAGAAG
GCCTTCAAGCAGTACGCCAATGACAACGGCGTGGATGGCGTGTGGACCTA
TGACGACGCCACGAAGACATTCACTGTGACTGAGATCGATGAGAACCTCT
ACTTCCAGGGCGGCAGTATGGACGAGAAGACCACCGGCTGGAGAGGCGGC
CACGTGGTCGAGGGACTGGCCGGTGAGCTGGAGCAGCTACGGGCCCGCCT
TGAGCACCACCCCCAGGGGCAGCGCGAGCCGGGCGGAGGCGGAGGCGGAT
CC

Amino Acid Sequence

View Sequence

MGTPAVTTYKLVINGKTLKGETTTKAVDAETAEKAFKQYANDNGVDGVWT
YDDATKTFTVTEVNTPAVTTYKLVINGKTLKGETTTKAVDAETAEKAFKQ
YANDNGVDGVWTYDDATKTFTVTEIDENLYFQGGSMDEKTTGWRGGHVVE
GLAGELEQLRARLEHHPQGQREPGGGGGGSMQIFVRTLTGRTITLEVEPS
DTIENVRARIQDREGIPPDQQRLIFAGRQLEDGRTLSDYNIQRESTLHLV
LRLRGG**AA

Vector Type

Mammalian

Antibiotic

Amp

Comments

*Swapped 6HIS-Gly linker Hind-Bam fragment of SC20446 for GS TAP tag-Gly linker.
NB. Since KallR mutant has internal BamHI site at nt91 subsequently restored full-length by subcloning BamHI flanked 91bp fragment from SC20447 to create final construct.
Flp-In vector for mammalian expression of N-terminally GS TAP tagged KallR ubiquitin (with initiating methionine)
SC20447

Estimated MW of expressed protein : 27910 Daltons

100042 R 1000 1001 1047 19007

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