Ubiquitin

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Plasmid
pET156P-Ub2 FRET donor
Parent Plasmid
pET15b 6His 3C
Expressed
6His-Ubiquitin 1-75 K48R K63R G75D
DU Number
DU20517
Genbank
P62988
Tag
6His
Species
Human
Sequence of Insert
GGATCCATGCAGATCTTCGTGAAGACCCTGACTGGTAAGACCATCACTCT
CGAAGTGGAGCCGAGTGACACCATTGAGAATGTCAAGGCAAAGATCCAAG
ACAAGGAAGGCATCCCTCCTGACCAGCAGAGGTTGATCTTTGCTGGGAGA
CAGCTGGAAGATGGACGCACCCTGTCTGACTACAACATCCAGAGAGAGTC
CACCCTGCACCTGGTCCTCCGTCTCAGAGATTGAGCGGCCGC
Amino Acid Sequence

MGSSHHHHHHSSGLEVLFQGPGSMQIFVKTLTGKTITLEVEPSDTIENVK
AKIQDKEGIPPDQQRLIFAGRQLEDGRTLSDYNIQRESTLHLVLRLRD*





Vector Type
Bacterial
Antibiotic
Amp
Comments
Bacterial expression construct to express 6HIS- Ubiquitin 1-75 K48R K63R G75D (contains preScission protease site)

Estimated MW of expressed protein : 11067 Daltons
100042 R 1000 1001 1047 19007
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