Fan1

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Plasmid
pGEX6P-1-Fan1 UBZ domain monomer (+ start and stop)
Parent Plasmid
pGEX6P1
Expressed
GST-C3-Fan1 UBZ domain
DU Number
DU20707
Genbank
NM_014967.3 GI:62198230
Tag
GST
Species
Human
Sequence of Insert
GGATCCATGGCCGGCAAACTTGCCTGCCCCGTTTGCAGTAAAATGGTGCC
TAGATATGACTTAAACCGGCACCTTGATGAAATGTGTGCTAACAATGACT
TCTGAGGCGCCGCGGCCGC
Amino Acid Sequence
MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMAGKLACPVCSKMVPRYDL
NRHLDEMCANNDF*

Vector Type
Bacterial
Antibiotic
Amp
Comments
Bacterial expression construct for GST-Fan1 UBZ domain monomer
Also acts as Intermediate for construction of UBZ domain concatamers

Estimated MW of expressed protein : 30468 Daltons
parental SC20103 (pGEX6P-1 minus Sfo1 site)
100042 R 1000 1001 1047 19007
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