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Synonyms
SUMO-1 Activating Enzyme E1 N Subunit
Plasmid
pGEX-4T-2 SAE1
Parent Plasmid
pGEX4T2
Expressed
GST SAE1
DU Number
DU20243
Tag
GST
Species
Human
Sequence of Insert
View Sequence
GGATCCATGGTGGAGAAGGAGGAGGCTGGCGGCGGCATTAGCGAGGAGGA
GGCGGCACAGTATGACCGGCAGATCCGCCTGTGGGGACTGGAGGCCCAGA
AACGGCTGCGGGCCTCTCGGGTGCTTCTTGTCGGCTTGAAAGGACTTGGG
GCTGAAATTGCCAAGAATCTCATCTTGGCAGGAGTGAAAGGACTGACCAT
GCTGGATCACGAACAGGTAACTCCAGAAGATCCCGGAGCTCAGTTCTTGA
TTCGTACTGGGTCTGTTGGCCGAAATAGGGCTGAAGCCTCTTTGGAGCGA
GCTCAGAATCTCAACCCCATGGTGGATGTGAAGGTGGACACTGAGGATAT
AGAGAAGAAACCAGAGTCATTTTTCACTCAATTCGATGCTGTGTGTCTGA
CTTGCTGCTCCAGGGATGTCATAGTTAAAGTTGACCAGATCTGTCACAAA
AATAGCATCAAGTTCTTTACAGGAGATGTTTTTGGCTACCATGGATACAC
ATTTGCCAATCTAGGAGAGCATGAGTTTGTAGAGGAGAAAACTAAAGTTG
CCAAAGTTAGCCAAGGAGTAGAAGATGGGCCCGACACCAAGAGAGCAAAA
CTTGATTCTTCTGAGACAACGATGGTCAAAAAGAAGGTGGTCTTCTGCCC
TGTTAAAGAAGCCCTGGAGGTGGACTGGAGCAGTGAGAAAGCAAAGGCTG
CTCTGAAGCGCACGACCTCCGACTACTTTCTCCTTCAAGTGCTCTTAAAG
TTCCGTACAGATAAAGGAAGAGATCCCAGTTCTGATACATATGAGGAAGA
TTCTGAGTTGTTGCTCCAGATACGAAATGATGTGCTTGACTCACTGGGTA
TTAGTCCTGACCTGCTTCCTGAGGACTTTGTCAGGTACTGCTTCTCCGAG
ATGGCCCCAGTGTGTGCGGTGGTTGGAGGGATTTTGGCACAGGAAATTGT
GAAGGCCCTGTCTCAGCGGGACCCTCCTCACAACAACTTCTTCTTCTTCG
ATGGCATGAAGGGGAATGGGATTGTGGAGTGCCTTGGCCCCAAGTGAGCG
GCCGC
GGCGGCACAGTATGACCGGCAGATCCGCCTGTGGGGACTGGAGGCCCAGA
AACGGCTGCGGGCCTCTCGGGTGCTTCTTGTCGGCTTGAAAGGACTTGGG
GCTGAAATTGCCAAGAATCTCATCTTGGCAGGAGTGAAAGGACTGACCAT
GCTGGATCACGAACAGGTAACTCCAGAAGATCCCGGAGCTCAGTTCTTGA
TTCGTACTGGGTCTGTTGGCCGAAATAGGGCTGAAGCCTCTTTGGAGCGA
GCTCAGAATCTCAACCCCATGGTGGATGTGAAGGTGGACACTGAGGATAT
AGAGAAGAAACCAGAGTCATTTTTCACTCAATTCGATGCTGTGTGTCTGA
CTTGCTGCTCCAGGGATGTCATAGTTAAAGTTGACCAGATCTGTCACAAA
AATAGCATCAAGTTCTTTACAGGAGATGTTTTTGGCTACCATGGATACAC
ATTTGCCAATCTAGGAGAGCATGAGTTTGTAGAGGAGAAAACTAAAGTTG
CCAAAGTTAGCCAAGGAGTAGAAGATGGGCCCGACACCAAGAGAGCAAAA
CTTGATTCTTCTGAGACAACGATGGTCAAAAAGAAGGTGGTCTTCTGCCC
TGTTAAAGAAGCCCTGGAGGTGGACTGGAGCAGTGAGAAAGCAAAGGCTG
CTCTGAAGCGCACGACCTCCGACTACTTTCTCCTTCAAGTGCTCTTAAAG
TTCCGTACAGATAAAGGAAGAGATCCCAGTTCTGATACATATGAGGAAGA
TTCTGAGTTGTTGCTCCAGATACGAAATGATGTGCTTGACTCACTGGGTA
TTAGTCCTGACCTGCTTCCTGAGGACTTTGTCAGGTACTGCTTCTCCGAG
ATGGCCCCAGTGTGTGCGGTGGTTGGAGGGATTTTGGCACAGGAAATTGT
GAAGGCCCTGTCTCAGCGGGACCCTCCTCACAACAACTTCTTCTTCTTCG
ATGGCATGAAGGGGAATGGGATTGTGGAGTGCCTTGGCCCCAAGTGAGCG
GCCGC
Amino Acid Sequence
View Sequence
MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSMVEKEEAGGGISEEEAAQYDRQIR
LWGLEAQKRLRASRVLLVGLKGLGAEIAKNLILAGVKGLTMLDHEQVTPE
DPGAQFLIRTGSVGRNRAEASLERAQNLNPMVDVKVDTEDIEKKPESFFT
QFDAVCLTCCSRDVIVKVDQICHKNSIKFFTGDVFGYHGYTFANLGEHEF
VEEKTKVAKVSQGVEDGPDTKRAKLDSSETTMVKKKVVFCPVKEALEVDW
SSEKAKAALKRTTSDYFLLQVLLKFRTDKGRDPSSDTYEEDSELLLQIRN
DVLDSLGISPDLLPEDFVRYCFSEMAPVCAVVGGILAQEIVKALSQRDPP
HNNFFFFDGMKGNGIVECLGPK
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSMVEKEEAGGGISEEEAAQYDRQIR
LWGLEAQKRLRASRVLLVGLKGLGAEIAKNLILAGVKGLTMLDHEQVTPE
DPGAQFLIRTGSVGRNRAEASLERAQNLNPMVDVKVDTEDIEKKPESFFT
QFDAVCLTCCSRDVIVKVDQICHKNSIKFFTGDVFGYHGYTFANLGEHEF
VEEKTKVAKVSQGVEDGPDTKRAKLDSSETTMVKKKVVFCPVKEALEVDW
SSEKAKAALKRTTSDYFLLQVLLKFRTDKGRDPSSDTYEEDSELLLQIRN
DVLDSLGISPDLLPEDFVRYCFSEMAPVCAVVGGILAQEIVKALSQRDPP
HNNFFFFDGMKGNGIVECLGPK
Vector Type
Bacterial
Comments
Estimated MW of expressed protein : 64733.10
100042 R 1000 1001 1047 19007
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