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Plasmid
pGEX6P-1-Ubiquitin K11R K48R K63R
Parent Plasmid
pGEX6P1
Expressed
GST-Ubiquitin K11R K48R K63R
DU Number
DU12216
Tag
GST
Species
Human
Sequence of Insert
View Sequence
GGATCCCAGATCTTCGTGAAGACCCTGACTGGTAGGACCATCACTCTCGA
AGTGGAGCCGAGTGACACCATTGAGAATGTCAAGGCAAAGATCCAAGACA
AGGAAGGCATCCCTCCTGACCAGCAGAGGTTGATCTTTGCTGGGAGACAG
CTGGAAGATGGACGCACCCTGTCTGACTACAACATCCAGAGAGAGTCCAC
CCTGCACCTGGTCCTCCGTCTCAGAGGTGGGTGATAACTCGAC
AGTGGAGCCGAGTGACACCATTGAGAATGTCAAGGCAAAGATCCAAGACA
AGGAAGGCATCCCTCCTGACCAGCAGAGGTTGATCTTTGCTGGGAGACAG
CTGGAAGATGGACGCACCCTGTCTGACTACAACATCCAGAGAGAGTCCAC
CCTGCACCTGGTCCTCCGTCTCAGAGGTGGGTGATAACTCGAC
Amino Acid Sequence
View Sequence
MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSQIFVKTLTGRTITLEVEPS
DTIENVKAKIQDKEGIPPDQQRLIFAGRQLEDGRTLSDYNIQRESTLHLV
LRLRGG
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSQIFVKTLTGRTITLEVEPS
DTIENVKAKIQDKEGIPPDQQRLIFAGRQLEDGRTLSDYNIQRESTLHLV
LRLRGG
Vector Type
Bacterial
Antibiotic
Amp
Comments
N terminal GST for bacterial expression.
100042 R 1000 1001 1047 19007
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