TRIM25 | Ubiquitylation Resource

Synonyms

*

Plasmid

pCMV-HA-2 TRIM 25

Parent Plasmid

pCMV5 HA2

Expressed

HA-TRIM 25

DU Number

DU12451

Genbank

NP_005073.2

Tag

HA

Species

Human

Sequence of Insert

View Sequence

GCGGCCGCGATGGCAGAGCTGTGCCCCCTGGCCGAGGAGCTGTCGTGCTC
CATCTGCCTGGAGCCCTTCAAGGAGCCGGTCACCACTCCGTGCGGCCACA
ACTTCTGCGGGTCGTGCCTGAATGAGACGTGGGCAGTCCAGGGCTCGCCA
TACCTGTGCCCGCAGTGCCGCGCCGTCTACCAGGCGCGACCGCAGCTGCA
CAAGAACACGGTGCTGTGCAACGTGGTGGAGCAGTTCCTGCAGGCCGACC
TGGCCCGGGAGCCACCCGCCGACGTCTGGACGCCGCCCGCCCGCGCCTCT
GCACCCAGCCCGAATGCCCAGGTGGCCTGCGACCACTGCCTGAAGGAGGC
CGCCGTGAAGACGTGCTTGGTGTGCATGGCCTCCTTCTGTCAGGAGCACC
TGCAGCCGCACTTCGACAGCCCCGCCTTCCAGGACCACCCGCTGCAGCCG
CCCGTTCGCGACCTGTTGCGCCGCAAATGTTCCCAGCACAATCGGCTGCG
GGAATTTTTCTGCCCCGAGCACAGCGAGTGCATCTGCCACATCTGCCTGG
TGGAGCATAAGACCTGCTCTCCCGCGTCCCTGAGCCAGGCCAGCGCCGAC
CTGGAGGCCACCCTGAGGCACAAACTAACTGTCATGTACAGTCAGATCAA
CGGGGCGTCGAGAGCACTGGATGATGTGAGAAACAGGCAGCAGGATGTGC
GGATGACTGCAAACAGAAAGGTGGAGCAGCTACAACAAGAATACACGGAA
ATGAAGGCTCTCTTGGACGCCTCAGAGACCACCTCGACAAGGAAGATAAA
GGAAGAGGAGAAGAGGGTCAACAGCAAGTTTGACACCATTTATCAGATTC
TCCTCAAGAAGAAGAGTGAGATCCAGACCTTGAAGGAGGAGATTGAACAG
AGCCTGACCAAGAGGGATGAGTTCGAGTTTCTGGAGAAAGCATCAAAACT
GCGAGGAATCTCAACAAAGCCAGTCTACATCCCCGAGGTGGAACTGAACC
ACAAGCTGATAAAAGGCATCCACCAGAGCACCATAGACCTCAAAAACGAG
CTGAAGCAGTGCATCGGGCGGCTCCAGGAGCTCACCCCCAGTTCAGGTGA
CCCTGGAGAGCATGACCCAGCGTCCACACACAAATCCACACGCCCTGTGA
AGAAGGTCTCCAAAGAGGAAAAGAAATCCAAGAAACCTCCCCCTGTCCCT
GCCTTACCCAGCAAGCTTCCCACGTTTGGAGCCCCGGAACAGTTAGTGGA
TTTAAAACAAGCTGGCTTGGAGGCTGCAGCCAAAGCCACCAGCTCACATC
CGAACTCAACATCTCTCAAGGCCAAGGTGCTGGAGACCTTCCTGGCCAAG
TCCAGACCTGAGCTCCTGGAGTATTACATTAAAGTCATCCTGGACTACAA
CACCGCCCACAACAAAGTGGCTCTGTCAGAGTGCTATACAGTAGCTTCTG
TGGCTGAGATGCCTCAGAACTACCGGCCGCATCCCCAGAGGTTCACATAC
TGCTCTCAGGTGCTGGGCCTGCACTGCTACAAGAAGGGGATCCACTACTG
GGAGGTGGAGCTGCAGAAGAACAACTTCTGTGGGGTAGGCATCTGCTACG
GAAGCATGAACCGGCAGGGCCCAGAAAGCAGGCTCGGCCGCAACAGCGCC
TCCTGGTGCGTGGAGTGGTTCAACACCAAGATCTCTGCCTGGCACAATAA
CGTGGAGAAAACCCTGCCCTCCACCAAGGCCACGCGGGTGGGCGTGCTTC
TCAACTGTGACCACGGCTTTGTCATCTTCTTCGCTGTTGCCGACAAGGTC
CACCTGATGTATAAGTTCAGGGTGGACTTTACTGAGGCTTTGTACCCGGC
TTTCTGGGTATTTTCTGCTGGTGCCACACTCTCCATCTGCTCCCCCAAGT
AGGCGGCCGC

Amino Acid Sequence

View Sequence

MYPYDVPDYAGIPGIPGSTRAAAMAELCPLAEELSCSICLEPFKEPVTTP
CGHNFCGSCLNETWAVQGSPYLCPQCRAVYQARPQLHKNTVLCNVVEQFL
QADLAREPPADVWTPPARASAPSPNAQVACDHCLKEAAVKTCLVCMASFC
QEHLQPHFDSPAFQDHPLQPPVRDLLRRKCSQHNRLREFFCPEHSECICH
ICLVEHKTCSPASLSQASADLEATLRHKLTVMYSQINGASRALDDVRNRQ
QDVRMTANRKVEQLQQEYTEMKALLDASETTSTRKIKEEEKRVNSKFDTI
YQILLKKKSEIQTLKEEIEQSLTKRDEFEFLEKASKLRGISTKPVYIPEV
ELNHKLIKGIHQSTIDLKNELKQCIGRLQELTPSSGDPGEHDPASTHKST
RPVKKVSKEEKKSKKPPPVPALPSKLPTFGAPEQLVDLKQAGLEAAAKAT
SSHPNSTSLKAKVLETFLAKSRPELLEYYIKVILDYNTAHNKVALSECYT
VASVAEMPQNYRPHPQRFTYCSQVLGLHCYKKGIHYWEVELQKNNFCGVG
ICYGSMNRQGPESRLGRNSASWCVEWFNTKISAWHNNVEKTLPSTKATRV
GVLLNCDHGFVIFFAVADKVHLMYKFRVDFTEALYPAFWVFSAGATLSIC
SPK*

Vector Type

Mammalian

Antibiotic

Amp

Comments

N ter HA for mammalian expression.

Estimated MW of expressed protein : 73351 Daltons

100042 R 1000 1001 1047 19007