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Plasmid
pET156P-1 UBE2B
Parent Plasmid
pET15b 6His 3C
Expressed
6His UBE2B
DU Number
DU32555
Genbank
NP_003328
Tag
6His
Species
Human
Sequence of Insert
View Sequence
ATGTCCCCTATACTAGGTTATTGGAAAATTAAGGGCCTTGTGCAACCCAC
TCGACTTCTTTTGGAATATCTTGAAGAAAAATATGAAGAGCATTTGTATG
AGCGCGATGAAGGTGATAAATGGCGAAACAAAAAGTTTGAATTGGGTTTG
GAGTTTCCCAATCTTCCTTATTATATTGATGGTGATGTTAAATTAACACA
GTCTATGGCCATCATACGTTATATAGCTGACAAGCACAACATGTTGGGTG
GTTGTCCAAAAGAGCGTGCAGAGATTTCAATGCTTGAAGGAGCGGTTTTG
GATATTAGATACGGTGTTTCGAGAATTGCATATAGTAAAGACTTTGAAAC
TCTCAAAGTTGATTTTCTTAGCAAGCTACCTGAAATGCTGAAAATGTTCG
AAGATCGTTTATGTCATAAAACATATTTAAATGGTGATCATGTAACCCAT
CCTGACTTCATGTTGTATGACGCTCTTGATGTTGTTTTATACATGGACCC
AATGTGCCTGGATGCGTTCCCAAAATTAGTTTGTTTTAAAAAACGTATTG
AAGCTATCCCACAAATTGATAAGTACTTGAAATCCAGCAAGTATATAGCA
TGGCCTTTGCAGGGCTGGCAAGCCACGTTTGGTGGTGGCGACCATCCTCC
AAAATCGGATCTGGAAGTTCTGTTCCAGGGGCCCCTGGGATCCTCGACCC
CGGCCCGGAGGAGGCTCATGCGGGATTTCAAGCGGTTACAAGAGGACCCA
CCTGTGGGTGTCAGTGGCGCACCATCTGAAAACAACATCATGCAGTGGAA
TGCAGTTATATTTGGACCAGAAGGGACACCTTTTGAAGATGGTACTTTTA
AACTAGTAATAGAATTTTCTGAAGAATATCCAAATAAACCACCAACTGTT
AGGTTTTTATCCAAAATGTTTCATCCAAATGTGTATGCTGATGGTAGCAT
ATGTTTAGATATCCTTCAGAATCGATGGAGTCCAACATATGATGTATCTT
CTATCTTAACATCAATTCAGTCTCTGCTGGATGAACCGAATCCTAACAGT
CCAGCCAATAGCCAGGCAGCACAGCTTTATCAGGAAAACAAACGAGAATA
TGAGAAAAGAGTTTCGGCCATTGTTGAACAAAGCTGGAATGATTCATAAG
CGGCCAAGGGCGAATTCC
TCGACTTCTTTTGGAATATCTTGAAGAAAAATATGAAGAGCATTTGTATG
AGCGCGATGAAGGTGATAAATGGCGAAACAAAAAGTTTGAATTGGGTTTG
GAGTTTCCCAATCTTCCTTATTATATTGATGGTGATGTTAAATTAACACA
GTCTATGGCCATCATACGTTATATAGCTGACAAGCACAACATGTTGGGTG
GTTGTCCAAAAGAGCGTGCAGAGATTTCAATGCTTGAAGGAGCGGTTTTG
GATATTAGATACGGTGTTTCGAGAATTGCATATAGTAAAGACTTTGAAAC
TCTCAAAGTTGATTTTCTTAGCAAGCTACCTGAAATGCTGAAAATGTTCG
AAGATCGTTTATGTCATAAAACATATTTAAATGGTGATCATGTAACCCAT
CCTGACTTCATGTTGTATGACGCTCTTGATGTTGTTTTATACATGGACCC
AATGTGCCTGGATGCGTTCCCAAAATTAGTTTGTTTTAAAAAACGTATTG
AAGCTATCCCACAAATTGATAAGTACTTGAAATCCAGCAAGTATATAGCA
TGGCCTTTGCAGGGCTGGCAAGCCACGTTTGGTGGTGGCGACCATCCTCC
AAAATCGGATCTGGAAGTTCTGTTCCAGGGGCCCCTGGGATCCTCGACCC
CGGCCCGGAGGAGGCTCATGCGGGATTTCAAGCGGTTACAAGAGGACCCA
CCTGTGGGTGTCAGTGGCGCACCATCTGAAAACAACATCATGCAGTGGAA
TGCAGTTATATTTGGACCAGAAGGGACACCTTTTGAAGATGGTACTTTTA
AACTAGTAATAGAATTTTCTGAAGAATATCCAAATAAACCACCAACTGTT
AGGTTTTTATCCAAAATGTTTCATCCAAATGTGTATGCTGATGGTAGCAT
ATGTTTAGATATCCTTCAGAATCGATGGAGTCCAACATATGATGTATCTT
CTATCTTAACATCAATTCAGTCTCTGCTGGATGAACCGAATCCTAACAGT
CCAGCCAATAGCCAGGCAGCACAGCTTTATCAGGAAAACAAACGAGAATA
TGAGAAAAGAGTTTCGGCCATTGTTGAACAAAGCTGGAATGATTCATAAG
CGGCCAAGGGCGAATTCC
Amino Acid Sequence
View Sequence
MYHHHHHSSGLEVLFQGPLGSSTPARRRLMRDFKRLQEDPPVGVSGAPSE
NNIMQWNAVIFGPEGTPFEDGTFKLVIEFSEEYPNKPPTVRFLSKMFHPN
VYADGSICLDILQNRWSPTYDVSSILTSIQSLLDEPNPNSPANSQAAQLY
QENKREYEKRVSAIVEQSWNDS*
NNIMQWNAVIFGPEGTPFEDGTFKLVIEFSEEYPNKPPTVRFLSKMFHPN
VYADGSICLDILQNRWSPTYDVSSILTSIQSLLDEPNPNSPANSQAAQLY
QENKREYEKRVSAIVEQSWNDS*
Vector Type
Bacterial
Antibiotic
Amp
Comments
Bacterial expression with N terminal 6HIS
100042 R 1000 1001 1047 19007
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